Detailed information NTG0730436

Bioinformatically predicted

Overview

• Name: chiS
• Type: Regulator
• Locus tag: N5E84_RS10060
• Genome accession: NZ_CP104554
• Coordinates: 2185483..2188875 (+)
• Length: 1130 a.a.
• NCBI ID: WP_029388441.1
• Uniprot ID: A0A289GER5
• Organism: Vibrio sp. J502
• Function: chitin sensor (predicted from homology) Competence regulation

Genomic Context

Location: 2180483..2193875

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N5E84_RS10040 (N5E84_10040)	erpA	2180834..2181175 (-)	342	WP_026027518.1	iron-sulfur cluster insertion protein ErpA	-
N5E84_RS10045 (N5E84_10045)	hemL	2181498..2182793 (+)	1296	WP_029388440.1	glutamate-1-semialdehyde 2,1-aminomutase	-
N5E84_RS10050 (N5E84_10050)	-	2183071..2184156 (+)	1086	WP_017045856.1	AI-2E family transporter	-
N5E84_RS10055 (N5E84_10055)	rsmC	2184240..2185283 (+)	1044	WP_019283245.1	16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC	-
N5E84_RS10060 (N5E84_10060)	chiS	2185483..2188875 (+)	3393	WP_029388441.1	response regulator	Regulator
N5E84_RS10065 (N5E84_10065)	-	2189620..2191290 (+)	1671	WP_069212218.1	ABC transporter substrate-binding protein	-
N5E84_RS10070 (N5E84_10070)	-	2191457..2192443 (+)	987	WP_013857640.1	ABC transporter permease	-
N5E84_RS10075 (N5E84_10075)	-	2192446..2193471 (+)	1026	WP_013857641.1	ABC transporter permease	-

Sequence

Protein

• Length: 1130 a.a.
• Molecular weight: 127839.55 Da
• Isoelectric Point: 6.2690

>NTDB_id=730436 N5E84_RS10060 WP_029388441.1 2185483..2188875(+) (chiS) [Vibrio sp. J502]
MFRFSRKQKFKRLQNTLMLAFLVLSITPLTVTAIFFLQSHTKDLQEQSTSHLLSVRDTKQQQVLDYLTAKESEVMGFVRS
ELAYASGGRFYGLVNAFQRLGLDIDQAREHAQQRYIPGSGDQIKTSILPQSSSYVGSERYRLLHKRYHWAYLELLKRSDF
DDILLVDLDGDVVYSVYKHDNYGTNLLTGKYKDSNLGKTFRQLEQQVGEQRKINEEYTPVIISDFSQQEGQSVAWLGAPI
IQQGYLHSYAMFRLPNNGITKLVADTNATSQIRTLLVGQDHHSRILSYTQQEIETSQKVINLALSGLSAVGTYTNTLNEK
TIAAYAPVANKNINWALVVEILEKEAYARVHQLEKLFIFAMLTAIILVVIASHYLSNFITSPLLKLTWAAEKVSAGDLDE
NVINTDRKDEIGRLAVSFERMQRSIREKMQLIKAQNKELESNIQLIRKQNDELHLANKLKDEFLATTSHELRTPLHGMVG
IAEALISGSSGTITADHRYQLGIIISSGQRLTNLVDDLLDYHKMRYGSLDIKQCAVDLSGATRLVLELSNHLLNKKPIRV
INQIPDECIWVSADPQRLEQVLYNLVGNAIKYTTEGKIVISANVVDDKIRVQVVDTGQGIPAEQLEHIFEPLIQAGQDAS
RYRQGAGLGLSISRQLIELMQGTLYVSSQPMVGTTFSFTLPLASKAEIESTSYMEAGHFQSPTISDIELPEMHSLPENKE
GPVLLIADDEPVNLRVLDSFLRLEGYQVRTAKDGNEVISAVEKEKPELLLLDIMMPGLSGYQVCEKLRETYDHAELPIIM
LTALNQTDDRVRGFAAGANDYLSKPFNKQELAARITAHLTASKAEQRRIENNQLQQELKHRAMVEASLLETQGRLLEQLE
SAPEAIICIRDDHKIRFANEAAARLFKRSQEQLKRSNAEELIAPKYLVVEQEHYCGNIDIYVEDTRQHMSADVLRLPDGS
GLQAMYIFNVGGNINAARINNLETAIEALSSYAFEGDKEKLQQLKELGGEFTRIADKVSGESQSKQDLMREVLVEAMTSA
LDYWERVSNQSKFTFAEQSGLWRVYLDRSTLQTRTLDKYLRIETLPKTPRWRTVLNSLDYILDHCQEHGPERSHIEAQRD
KLQKLLTSSL

Nucleotide

• Length: 3393 bp

>NTDB_id=730436 N5E84_RS10060 WP_029388441.1 2185483..2188875(+) (chiS) [Vibrio sp. J502]
ATGTTTAGGTTCTCTCGTAAGCAAAAATTTAAGCGCCTGCAAAATACTCTTATGCTGGCTTTTCTCGTTTTGAGCATCAC
GCCTTTAACTGTGACTGCCATTTTTTTTCTGCAATCACACACTAAAGATCTTCAAGAACAAAGCACGTCTCACTTATTGT
CAGTCAGAGACACTAAGCAACAGCAAGTTCTCGATTACCTCACGGCCAAAGAATCAGAAGTGATGGGGTTTGTCCGTTCA
GAGTTAGCTTATGCTAGTGGAGGGCGCTTTTACGGCTTAGTCAATGCGTTCCAAAGACTTGGTCTGGATATTGATCAAGC
TCGTGAGCATGCCCAGCAGCGTTATATTCCTGGCTCTGGTGATCAGATAAAAACGTCAATATTGCCACAATCGAGCAGCT
ATGTAGGCAGTGAACGGTATCGCCTACTGCACAAACGCTATCATTGGGCGTATTTAGAACTGCTTAAACGTTCTGATTTT
GACGATATTTTACTGGTGGATCTCGATGGTGATGTCGTTTATTCCGTTTATAAGCACGACAACTATGGGACGAATTTACT
GACCGGAAAATACAAAGATAGCAATCTGGGTAAAACATTCCGCCAGTTAGAGCAGCAAGTTGGCGAACAACGAAAAATCA
ATGAGGAATATACACCAGTCATTATTTCTGATTTTTCACAACAAGAAGGGCAATCAGTTGCTTGGCTTGGCGCGCCCATT
ATTCAACAGGGCTACCTGCACAGCTACGCCATGTTCCGCCTACCAAATAATGGCATCACAAAGCTCGTTGCTGACACCAA
TGCGACATCGCAAATAAGAACTTTACTCGTCGGGCAAGATCATCATTCTCGAATACTCTCTTACACGCAGCAAGAAATCG
AGACTAGTCAAAAAGTGATCAATCTTGCGCTTTCTGGATTGAGTGCCGTTGGTACTTACACTAATACATTGAATGAGAAA
ACCATTGCCGCCTATGCACCTGTTGCAAACAAAAACATCAATTGGGCCTTGGTCGTAGAGATACTAGAAAAAGAGGCTTA
TGCGCGAGTTCATCAGCTTGAGAAGTTGTTTATTTTCGCCATGCTAACCGCGATTATTTTAGTCGTCATCGCTTCACATT
ATCTCTCTAATTTTATTACCTCTCCCCTACTGAAGCTCACTTGGGCGGCAGAAAAAGTCTCAGCTGGGGATTTAGATGAA
AACGTTATCAATACCGATCGTAAAGATGAGATTGGTCGGCTCGCAGTGAGTTTTGAACGTATGCAGCGTTCGATTCGAGA
AAAAATGCAGCTGATTAAAGCGCAAAATAAAGAGCTCGAAAGCAACATTCAATTAATCCGCAAACAGAACGATGAGTTGC
ATCTCGCTAACAAACTAAAAGATGAATTCCTAGCCACAACCTCACATGAATTAAGAACACCGTTGCACGGTATGGTCGGT
ATTGCAGAAGCGCTGATCTCTGGGTCAAGTGGCACGATCACCGCCGATCACCGTTATCAATTGGGCATAATCATCAGCAG
TGGACAACGGCTGACAAACTTGGTCGATGACTTACTCGATTATCATAAGATGCGCTATGGCAGCTTGGATATCAAACAGT
GTGCTGTTGATCTATCCGGTGCAACACGCTTAGTGTTAGAGCTGTCTAACCATCTACTCAACAAAAAGCCCATTCGCGTA
ATAAACCAAATCCCTGATGAATGCATTTGGGTCTCTGCCGATCCACAACGATTAGAGCAAGTGCTTTATAACCTTGTAGG
AAACGCCATTAAATATACGACTGAAGGGAAAATTGTCATTTCAGCCAATGTGGTTGATGACAAGATTCGTGTCCAAGTTG
TCGATACGGGGCAAGGCATTCCTGCTGAACAACTTGAACACATCTTCGAACCACTGATTCAAGCAGGGCAAGATGCCAGT
CGTTATCGACAAGGCGCAGGCTTAGGTCTTTCGATCAGCCGTCAATTGATCGAATTAATGCAAGGAACGCTTTATGTCAG
TAGCCAACCTATGGTCGGTACGACATTCAGTTTTACCTTACCATTAGCATCAAAAGCGGAAATTGAATCGACAAGCTATA
TGGAAGCTGGCCATTTCCAATCTCCGACCATTTCAGACATTGAACTGCCTGAAATGCATTCACTACCTGAAAATAAAGAA
GGCCCAGTGCTACTGATAGCAGATGATGAACCCGTCAATCTGCGTGTCCTAGACAGCTTCCTACGTTTAGAAGGCTATCA
AGTCCGCACCGCTAAAGATGGCAATGAAGTCATTAGCGCGGTCGAAAAAGAAAAACCGGAACTTCTACTGCTCGACATTA
TGATGCCAGGATTAAGCGGCTACCAAGTCTGTGAAAAGTTAAGAGAAACATACGATCATGCCGAGCTACCTATCATTATG
TTGACGGCATTAAATCAAACTGACGATAGAGTTCGTGGTTTTGCAGCGGGAGCGAACGATTACTTATCGAAGCCGTTTAA
TAAGCAAGAGCTCGCTGCACGTATTACGGCGCATTTAACGGCCAGTAAAGCTGAACAAAGACGTATTGAGAACAACCAAC
TGCAACAAGAGCTGAAGCATAGAGCAATGGTAGAGGCCAGTTTATTGGAAACCCAAGGCCGTCTACTAGAGCAGCTCGAA
TCCGCTCCTGAGGCCATTATTTGCATTCGAGACGATCATAAAATCCGCTTTGCGAACGAAGCGGCTGCTCGGTTATTTAA
GCGCAGCCAAGAACAACTAAAGCGCTCAAATGCGGAAGAACTCATCGCCCCCAAATACTTAGTCGTCGAGCAAGAGCATT
ACTGCGGTAATATCGATATCTATGTCGAAGATACACGTCAGCATATGTCTGCTGATGTGTTACGACTACCGGATGGCTCT
GGGTTGCAGGCTATGTACATCTTTAACGTGGGTGGAAACATCAATGCAGCGCGCATCAATAACCTTGAAACCGCTATTGA
AGCGCTATCCAGTTACGCATTTGAAGGCGATAAAGAAAAACTGCAACAGCTCAAAGAACTCGGTGGCGAGTTCACACGTA
TCGCCGACAAAGTATCGGGAGAGAGTCAATCTAAGCAAGATTTAATGCGCGAAGTACTGGTTGAAGCAATGACCAGTGCT
TTAGATTACTGGGAACGGGTCAGTAACCAAAGCAAGTTTACCTTTGCAGAGCAGAGTGGGCTATGGCGAGTATATCTCGA
TAGAAGCACATTACAGACTCGGACACTAGACAAGTACTTACGTATCGAGACACTGCCTAAAACACCTCGCTGGCGTACCG
TTCTTAATTCGTTAGATTACATTCTTGATCATTGTCAGGAGCACGGCCCTGAACGCTCTCACATCGAAGCGCAGCGCGAT
AAATTGCAAAAGCTACTGACCTCATCACTCTAA

Domains

Predicted by InterproScan.

• HAMP domain (HAMP) (375-427)

• Response regulator receiver domain (Response_reg) (725-835)

• His Kinase A (phospho-acceptor) domain (HisKA) (460-525)

• PAS fold (PAS) (876-926)

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (574-683)

3D structure

• AlphaFold DB: A0A289GER5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
chiS	Vibrio cholerae strain A1552	81.56	99.823	0.814