Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NQS35_RS02535 Genome accession   NZ_CP104494
Coordinates   524057..525556 (-) Length   499 a.a.
NCBI ID   WP_011000369.1    Uniprot ID   A0A223GJ62
Organism   Ralstonia pseudosolanacearum strain Cw717     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 519057..530556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQS35_RS02505 (NQS35_02485) - 519565..520215 (-) 651 WP_016724261.1 LON peptidase substrate-binding domain-containing protein -
  NQS35_RS02510 (NQS35_02490) rapZ 520293..521183 (-) 891 WP_016724262.1 RNase adapter RapZ -
  NQS35_RS02515 (NQS35_02495) - 521277..521612 (-) 336 WP_020425146.1 PsiF family protein -
  NQS35_RS02520 (NQS35_02500) hprK 521803..522777 (-) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  NQS35_RS02525 (NQS35_02505) ptsN 522848..523303 (-) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NQS35_RS02530 (NQS35_02510) hpf 523652..524005 (-) 354 WP_011000368.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  NQS35_RS02535 (NQS35_02515) rpoN/rpoN1 524057..525556 (-) 1500 WP_011000369.1 RNA polymerase factor sigma-54 Machinery gene
  NQS35_RS02540 (NQS35_02520) lptB 525654..526442 (-) 789 WP_016724264.1 LPS export ABC transporter ATP-binding protein -
  NQS35_RS02545 (NQS35_02525) lptA 526476..527051 (-) 576 WP_011000371.1 lipopolysaccharide transport periplasmic protein LptA -
  NQS35_RS02550 (NQS35_02530) lptC 527120..527752 (-) 633 WP_011000372.1 LPS export ABC transporter periplasmic protein LptC -
  NQS35_RS02555 (NQS35_02535) - 527754..528341 (-) 588 WP_011000373.1 KdsC family phosphatase -
  NQS35_RS02560 (NQS35_02540) - 528380..529363 (-) 984 WP_016724265.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55227.56 Da        Isoelectric Point: 4.8064

>NTDB_id=730021 NQS35_RS02535 WP_011000369.1 524057..525556(-) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain Cw717]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSSSATPLPTE
SPPPAANGADHADTAEASYENGSDFDSDYGTERSDWSLDDFARKPQSDEDDRAPMQLREAEPTQREFLMEQLAPLKLSPR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
TIVNQHLELLAARDYTRLKKALSVDDGELKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=730021 NQS35_RS02535 WP_011000369.1 524057..525556(-) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain Cw717]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCCCAGCATCTGGCGCTCACGCCACAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAGCTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTCGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGACGGCTCGGTCAACGCCCTGCAGAGCAGCAGCGCCACCCCGCTGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGCGCGGACCATGCCGACACGGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTACGGCACCGAGCGCTCCGACTGGAGCCTCGACGACTTCGCGCGCAAACCCCAGTCCGACGAAGACGACCGCGCGC
CGATGCAGCTGCGCGAGGCCGAACCCACGCAGCGCGAATTCCTGATGGAACAACTCGCGCCGCTCAAGCTGTCCCCGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAGCTCGACGAATTGCAGGCCGCGCTGCGGATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGCGCGCGCTCGGCGGCCGAATGCCTGACGCTGCAGCTGCAGCGCCTGGACTCCCCGGCCAAGCCGCTGGCGCTG
ACCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGGCTGAAGAAAGCGCTGTCCGTCGATGATGG
CGAGCTGAAGGCCGCGCATGAGCTGATCCGCTCGCTGGCGCCCTTTCCTGGCCACGCCTTCGGGCGGGCCGAGGCCGACT
TCGTCGTGCCCGACGTGGTGGTGCGCAAGACCGCCGCCGGCTGGATGGCACAGCTGAACCCCGACGTGATGCCACGCCTG
CGCATCAACGACATGTATGCGCAGATCCTGCGCAGCAGCCGTGGCGAATCGGGTGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCAATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAATACATGGCAACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAGATCCCAGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A223GJ62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

100

100

1