Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N4232_RS03875 Genome accession   NZ_CP104488
Coordinates   794580..795560 (+) Length   326 a.a.
NCBI ID   WP_001055647.1    Uniprot ID   A0A3V9PQV4
Organism   Salmonella enterica strain SalSpp_sample_10_No.2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 789580..800560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4232_RS03845 (N4232_03845) - 790158..791165 (+) 1008 WP_000252190.1 DUF1202 domain-containing protein -
  N4232_RS03850 (N4232_03850) hemW 791256..792392 (-) 1137 WP_001096527.1 radical SAM family heme chaperone HemW -
  N4232_RS03855 (N4232_03855) - 792385..792978 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  N4232_RS03860 (N4232_03860) yggU 792986..793276 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  N4232_RS03865 (N4232_03865) - 793273..793839 (-) 567 WP_001094848.1 YggT family protein -
  N4232_RS03870 (N4232_03870) - 793858..794562 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  N4232_RS03875 (N4232_03875) pilT 794580..795560 (+) 981 WP_001055647.1 type IV pilus twitching motility protein PilT Machinery gene
  N4232_RS03880 (N4232_03880) - 795688..796374 (+) 687 WP_000098324.1 IclR family transcriptional regulator -
  N4232_RS03885 (N4232_03885) ruvX 796421..796837 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  N4232_RS03890 (N4232_03890) - 796837..797400 (-) 564 WP_001053171.1 YqgE/AlgH family protein -
  N4232_RS03895 (N4232_03895) gshB 797616..798563 (-) 948 WP_000593248.1 glutathione synthase -
  N4232_RS03900 (N4232_03900) rsmE 798583..799314 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N4232_RS03905 (N4232_03905) endA 799391..800098 (-) 708 WP_000286123.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36151.32 Da        Isoelectric Point: 7.5116

>NTDB_id=729976 N4232_RS03875 WP_001055647.1 794580..795560(+) (pilT) [Salmonella enterica strain SalSpp_sample_10_No.2]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=729976 N4232_RS03875 WP_001055647.1 794580..795560(+) (pilT) [Salmonella enterica strain SalSpp_sample_10_No.2]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACATATCCTGACGCTGGAAGATCCTATTGAA
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCCGTACTATCGCAAAAACTGGAGGT
CGACAGGCAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3V9PQV4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Acinetobacter baylyi ADP1

45.566

100

0.457
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.556

100

0.371
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362