Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   LDO00_RS09760 Genome accession   NZ_AP024521
Coordinates   2017544..2018554 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain E189     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2012544..2023554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDO00_RS09740 (NIAHE189_18780) mepM 2013511..2014833 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  LDO00_RS09745 (NIAHE189_18790) znuA 2014849..2015781 (-) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  LDO00_RS09750 (NIAHE189_18800) znuC 2015860..2016615 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  LDO00_RS09755 (NIAHE189_18810) znuB 2016612..2017397 (+) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  LDO00_RS09760 (NIAHE189_18820) ruvB 2017544..2018554 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  LDO00_RS09765 (NIAHE189_18830) ruvA 2018563..2019174 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  LDO00_RS09770 yobI 2019313..2019378 (-) 66 WP_001386853.1 stress response small protein YobI -
  LDO00_RS09775 (NIAHE189_18840) yebB 2019449..2020051 (+) 603 WP_001024928.1 YebB family permuted papain-like enzyme -
  LDO00_RS09780 (NIAHE189_18850) ruvC 2020053..2020574 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  LDO00_RS09785 (NIAHE189_18860) yebC 2020609..2021349 (-) 741 WP_000907248.1 YebC/PmpR family DNA-binding transcriptional regulator -
  LDO00_RS09790 (NIAHE189_18870) nudB 2021378..2021830 (-) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=72975 LDO00_RS09760 WP_000568519.1 2017544..2018554(-) (ruvB) [Escherichia coli strain E189]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=72975 LDO00_RS09760 WP_000568519.1 2017544..2018554(-) (ruvB) [Escherichia coli strain E189]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATCGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGAGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment