Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N5F14_RS20655 Genome accession   NZ_CP104403
Coordinates   4261214..4262647 (+) Length   477 a.a.
NCBI ID   WP_133983259.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. steigerwaltii strain csa13R     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4256214..4267647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5F14_RS20625 (N5F14_20625) - 4256781..4257758 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  N5F14_RS20630 (N5F14_20630) kdsD 4257772..4258758 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  N5F14_RS20635 (N5F14_20635) kdsC 4258773..4259339 (+) 567 WP_017693909.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  N5F14_RS20640 (N5F14_20640) lptC 4259336..4259911 (+) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  N5F14_RS20645 (N5F14_20645) lptA 4259880..4260434 (+) 555 WP_062938089.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  N5F14_RS20650 (N5F14_20650) lptB 4260441..4261166 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  N5F14_RS20655 (N5F14_20655) rpoN/rpoN1 4261214..4262647 (+) 1434 WP_133983259.1 RNA polymerase factor sigma-54 Machinery gene
  N5F14_RS20660 (N5F14_20660) hpf 4262670..4262957 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  N5F14_RS20665 (N5F14_20665) ptsN 4263040..4263531 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  N5F14_RS20670 (N5F14_20670) rapZ 4263577..4264431 (+) 855 WP_003861870.1 RNase adapter RapZ -
  N5F14_RS20675 (N5F14_20675) npr 4264428..4264700 (+) 273 WP_133983261.1 PTS phosphocarrier protein NPr -
  N5F14_RS20680 (N5F14_20680) mtgA 4264740..4265465 (-) 726 WP_015571986.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  N5F14_RS20685 (N5F14_20685) elbB 4265462..4266115 (-) 654 WP_032655241.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54100.00 Da        Isoelectric Point: 4.3617

>NTDB_id=729636 N5F14_RS20655 WP_133983259.1 4261214..4262647(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. steigerwaltii strain csa13R]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEKKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=729636 N5F14_RS20655 WP_133983259.1 4261214..4262647(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. steigerwaltii strain csa13R]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAAAAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGCGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACCCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTTGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCTGTTCTGAAACGCGTTCAGCGTTTCGACCC
GATAGGTGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATTTGGATCTGCTGGCGAATCACGACTTCCGCACCCTGATGCGCGTCACACGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATCCCCGATGTGCTGGTGAGAAAACACAATGGCCGGTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCAGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48