Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N5F14_RS19020 Genome accession   NZ_CP104403
Coordinates   3937160..3938140 (-) Length   326 a.a.
NCBI ID   WP_033487646.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. steigerwaltii strain csa13R     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3932160..3943140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5F14_RS18990 (N5F14_18990) - 3933038..3933535 (+) 498 WP_017692693.1 SprT family zinc-dependent metalloprotease -
  N5F14_RS18995 (N5F14_18995) endA 3933630..3934337 (+) 708 WP_003860034.1 deoxyribonuclease I -
  N5F14_RS19000 (N5F14_19000) rsmE 3934389..3935120 (+) 732 WP_017692692.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N5F14_RS19005 (N5F14_19005) gshB 3935152..3936099 (+) 948 WP_015571835.1 glutathione synthase -
  N5F14_RS19010 (N5F14_19010) - 3936174..3936734 (+) 561 WP_006811924.1 YqgE/AlgH family protein -
  N5F14_RS19015 (N5F14_19015) ruvX 3936734..3937150 (+) 417 WP_033487645.1 Holliday junction resolvase RuvX -
  N5F14_RS19020 (N5F14_19020) pilT 3937160..3938140 (-) 981 WP_033487646.1 type IV pilus twitching motility protein PilT Machinery gene
  N5F14_RS19025 (N5F14_19025) - 3938158..3938859 (+) 702 WP_045347340.1 YggS family pyridoxal phosphate-dependent enzyme -
  N5F14_RS19030 (N5F14_19030) - 3938881..3939447 (+) 567 WP_015571838.1 YggT family protein -
  N5F14_RS19035 (N5F14_19035) yggU 3939444..3939740 (+) 297 WP_023304404.1 DUF167 family protein YggU -
  N5F14_RS19040 (N5F14_19040) - 3939744..3940337 (+) 594 WP_033487648.1 XTP/dITP diphosphatase -
  N5F14_RS19045 (N5F14_19045) hemW 3940330..3941478 (+) 1149 WP_017382926.1 radical SAM family heme chaperone HemW -
  N5F14_RS19050 (N5F14_19050) - 3941535..3941873 (+) 339 WP_017382927.1 endonuclease domain-containing protein -
  N5F14_RS19055 (N5F14_19055) - 3941956..3942672 (-) 717 WP_015571842.1 DUF2884 domain-containing protein -
  N5F14_RS19060 (N5F14_19060) - 3942729..3943055 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35681.72 Da        Isoelectric Point: 6.5000

>NTDB_id=729635 N5F14_RS19020 WP_033487646.1 3937160..3938140(-) (pilT) [Enterobacter hormaechei subsp. steigerwaltii strain csa13R]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNNEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMLGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=729635 N5F14_RS19020 WP_033487646.1 3937160..3938140(-) (pilT) [Enterobacter hormaechei subsp. steigerwaltii strain csa13R]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACA
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAAGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCTCGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAGCTGCTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCTATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCATACGCGCGGCGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

37.578

98.773

0.371