Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   N4S13_RS00740 Genome accession   NZ_CP104392
Coordinates   149858..151231 (+) Length   457 a.a.
NCBI ID   WP_010747276.1    Uniprot ID   R2NRU4
Organism   Enterococcus raffinosus strain HG-5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 144858..156231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4S13_RS00720 (N4S13_00720) - 145234..145980 (+) 747 WP_028020844.1 TIGR03915 family putative DNA repair protein -
  N4S13_RS00725 (N4S13_00725) - 146104..147060 (+) 957 WP_010747279.1 cation diffusion facilitator family transporter -
  N4S13_RS00730 (N4S13_00730) - 147330..149129 (+) 1800 WP_010747278.1 glycerophosphoryl diester phosphodiesterase membrane domain-containing protein -
  N4S13_RS00735 (N4S13_00735) - 149321..149827 (+) 507 WP_010747277.1 hypothetical protein -
  N4S13_RS00740 (N4S13_00740) radA 149858..151231 (+) 1374 WP_010747276.1 DNA repair protein RadA Machinery gene
  N4S13_RS00745 (N4S13_00745) - 151309..152463 (+) 1155 WP_010747275.1 PIN/TRAM domain-containing protein -
  N4S13_RS00750 (N4S13_00750) gltX 152561..154018 (+) 1458 WP_010747274.1 glutamate--tRNA ligase -
  N4S13_RS00755 (N4S13_00755) epsC 154637..155170 (+) 534 WP_010747273.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50036.52 Da        Isoelectric Point: 7.7029

>NTDB_id=729465 N4S13_RS00740 WP_010747276.1 149858..151231(+) (radA) [Enterococcus raffinosus strain HG-5]
MAKKSKTQFICQECGYVSPKYLGRCPNCGNWNTMVEEIEQNHPEDRRNRVSLTGERATPTKLKDVVFKKETRVQTELAEL
NRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSHQLAATGGTVLYVSGEESAQQIKLRAERLGSSETDFYLYAETDMGEIS
RTIENLKPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKLAKTNGIAIFIVGHVTKEGNIAGPRMLEHMVDTVLYF
EGEKHHSFRILRAVKNRFGSTNEIGIFEMRERGLVEVANPSQVFLEERLEGATGSAIVVAMEGSRPILVEVQALVTPTVF
GNAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAVAVAIASSYKENGTEATECFIGEIGLT
GEIRRVNSIEQRVKEASKLGFKKIYLPKNNMGDWKPPKGIELVPVATLGETLRKVFR

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=729465 N4S13_RS00740 WP_010747276.1 149858..151231(+) (radA) [Enterococcus raffinosus strain HG-5]
TTGGCAAAAAAATCCAAGACACAATTTATCTGCCAAGAATGTGGTTATGTTTCGCCTAAGTATTTAGGGCGTTGTCCTAA
CTGCGGAAATTGGAACACGATGGTAGAGGAGATTGAGCAAAATCATCCCGAAGATCGACGCAACCGTGTCAGTTTAACCG
GAGAGCGTGCGACACCTACCAAATTAAAGGATGTCGTCTTCAAAAAGGAAACGCGCGTTCAGACAGAATTAGCTGAATTG
AATCGCGTTCTTGGCGGCGGAGTGGTTCCAGGTTCATTGGTATTAATCGGTGGGGACCCAGGTATTGGGAAATCCACCTT
ACTATTGCAAGTATCCCATCAATTAGCGGCAACCGGCGGCACAGTTTTGTATGTTTCTGGAGAAGAAAGTGCGCAGCAGA
TAAAATTGCGTGCGGAGCGCCTGGGATCAAGTGAGACAGATTTTTATTTATATGCGGAAACAGACATGGGAGAAATCAGC
CGAACGATTGAAAATCTGAAGCCGGATTATGTGATCATCGACTCGATCCAAACCATGACACAACCAGATATCACTAGTGT
AGCGGGCTCGGTCAGCCAGGTTCGGGAGACCACCGCAGAGCTATTGAAGCTGGCGAAGACGAACGGCATTGCGATTTTTA
TCGTAGGTCATGTGACCAAAGAGGGAAATATCGCAGGTCCGCGAATGCTGGAGCATATGGTGGACACTGTTTTATACTTT
GAAGGCGAGAAGCATCACAGCTTTCGAATCTTACGAGCGGTAAAAAATCGTTTCGGTTCCACTAATGAAATCGGAATTTT
CGAGATGAGAGAACGGGGGCTGGTCGAAGTGGCCAATCCTTCACAGGTTTTCCTAGAGGAACGCCTAGAAGGGGCGACCG
GATCAGCAATCGTGGTTGCCATGGAAGGCAGCCGTCCGATACTGGTTGAGGTACAGGCATTGGTAACCCCCACTGTTTTC
GGCAACGCGAAGCGCACAACGACCGGCCTAGATTTCAACCGTGTTTCTTTGATCATGGCTGTTTTGGAAAAACGCGCCGG
GTTGCTGCTGCAAAATCAAGATGCCTACTTAAAAGCGGCAGGCGGGGTAAAATTAAATGAACCGGCCATTGATTTAGCGG
TGGCTGTGGCGATTGCCTCCAGCTACAAAGAAAATGGCACGGAAGCCACCGAATGCTTTATCGGTGAAATTGGTTTGACG
GGTGAAATTCGTCGGGTCAATAGCATTGAGCAGCGGGTGAAAGAAGCTAGCAAATTGGGCTTCAAAAAAATCTATCTGCC
TAAGAACAATATGGGCGATTGGAAACCGCCAAAAGGAATCGAATTGGTTCCTGTGGCAACCTTAGGGGAAACCTTAAGAA
AAGTATTTCGTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(57-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R2NRU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.123

99.781

0.74
  radA Streptococcus mitis SK321

73.904

99.781

0.737
  radA Streptococcus pneumoniae Rx1

73.904

99.781

0.737
  radA Streptococcus pneumoniae D39

73.904

99.781

0.737
  radA Streptococcus pneumoniae R6

73.904

99.781

0.737
  radA Streptococcus pneumoniae TIGR4

73.904

99.781

0.737
  radA Bacillus subtilis subsp. subtilis str. 168

64.967

98.687

0.641