Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N5B55_RS01500 Genome accession   NZ_CP104381
Coordinates   318732..320243 (-) Length   503 a.a.
NCBI ID   WP_304538917.1    Uniprot ID   -
Organism   Ralstonia pickettii strain MCR     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 313732..325243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5B55_RS01470 (N5B55_01470) - 314225..314878 (-) 654 WP_012761094.1 LON peptidase substrate-binding domain-containing protein -
  N5B55_RS01475 (N5B55_01475) rapZ 314978..315871 (-) 894 WP_012761095.1 RNase adapter RapZ -
  N5B55_RS01480 (N5B55_01480) - 315966..316295 (-) 330 WP_154206290.1 PsiF family protein -
  N5B55_RS01485 (N5B55_01485) hprK 316478..317452 (-) 975 WP_004633393.1 HPr(Ser) kinase/phosphatase -
  N5B55_RS01490 (N5B55_01490) ptsN 317526..317981 (-) 456 WP_012761097.1 PTS IIA-like nitrogen regulatory protein PtsN -
  N5B55_RS01495 (N5B55_01495) hpf 318328..318681 (-) 354 WP_004633388.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  N5B55_RS01500 (N5B55_01500) rpoN/rpoN1 318732..320243 (-) 1512 WP_304538917.1 RNA polymerase factor sigma-54 Machinery gene
  N5B55_RS01505 (N5B55_01505) lptB 320340..321128 (-) 789 WP_004633384.1 LPS export ABC transporter ATP-binding protein -
  N5B55_RS01510 (N5B55_01510) lptA 321159..321746 (-) 588 WP_154206292.1 lipopolysaccharide transport periplasmic protein LptA -
  N5B55_RS01515 (N5B55_01515) lptC 321805..322437 (-) 633 WP_012761100.1 LPS export ABC transporter periplasmic protein LptC -
  N5B55_RS01520 (N5B55_01520) - 322442..323026 (-) 585 WP_304538918.1 HAD family hydrolase -
  N5B55_RS01525 (N5B55_01525) - 323043..324026 (-) 984 WP_037027665.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 55726.05 Da        Isoelectric Point: 4.9039

>NTDB_id=729246 N5B55_RS01500 WP_304538917.1 318732..320243(-) (rpoN/rpoN1) [Ralstonia pickettii strain MCR]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSNSATPLPTE
SPPPAPNGGESSNSSDSSYENGHDNGADFDSDYGSDRSDWSLDDFARKPQSDEDDRAPLQLREAEQSLREFLMEQLAPLK
LSMRDKGLVIFLIESLDDEGYLSGTLDEILADLPTELEVELDELQAALRMLQSFDPPGIGARSAAECLSLQLHRLDSPAK
ALALVIVNQHLELLAARDYTRLKKALSVDEPALKAAHELIRSLTPFPGHAFGRAEADFVVPDVIVRKTSAGWMAQLNPDV
MPRLRINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADT
LGLHESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVV
ARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=729246 N5B55_RS01500 WP_304538917.1 318732..320243(-) (rpoN/rpoN1) [Ralstonia pickettii strain MCR]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTCGCGCTCACGCCGCAGTTGCAGCAATCGATCAGATTGCTGCA
GTTGTCGACCATCGAACTGCAGCAGGAAGTTGAACAGGCCCTCACCGAAAACCCGCTGCTGGAGCGTGAAAACGAGTGGC
TCGACACCTCCGCCCGCATTGGCGCAGACGGCTCCGTCAACGCGCTGCAAAGCAACAGCGCCACGCCCCTTCCCACTGAA
TCACCTCCGCCCGCACCCAACGGCGGCGAGAGCAGCAACAGCTCGGACAGCAGCTACGAAAACGGCCACGACAACGGTGC
CGATTTCGACAGCGATTACGGCTCCGACCGCTCCGACTGGAGCCTTGACGACTTCGCCCGCAAACCGCAATCCGATGAAG
ACGATCGTGCTCCGCTGCAGTTGCGCGAAGCCGAACAAAGTCTGCGCGAATTCCTCATGGAACAGCTCGCGCCGCTCAAG
CTCTCGATGCGCGACAAGGGCCTCGTCATCTTCCTGATCGAGTCGCTCGACGACGAAGGCTATCTGTCGGGCACGCTCGA
CGAAATCCTCGCAGACCTGCCGACCGAACTCGAAGTCGAACTGGACGAACTCCAAGCCGCCCTGCGCATGCTGCAGAGCT
TTGATCCACCGGGCATTGGCGCGCGCTCGGCAGCCGAATGCCTTTCGTTGCAACTGCATCGGCTCGACTCGCCCGCGAAG
GCGCTGGCGCTCGTCATCGTCAATCAGCATCTCGAACTGCTGGCCGCGCGCGACTACACGCGCCTGAAGAAGGCGCTGTC
CGTCGACGAGCCCGCGTTGAAGGCAGCGCATGAGCTGATTCGCTCACTGACACCATTCCCGGGTCATGCTTTCGGCCGTG
CTGAAGCAGATTTCGTGGTGCCCGACGTGATCGTGCGAAAAACGAGCGCAGGCTGGATGGCGCAACTGAACCCCGACGTC
ATGCCGCGCTTGCGCATCAACGACATGTACGCGCAGATTCTGCGCAGCAGCCGTGGCGAGTCGGGCGCCGCCAATCTGCA
GCAGAAGCTCCAAGAAGCACGTTGGCTGATCAAGAACATTCAACAGCGCTTCGATACGATCCTGCGTGTCTCGCAAGCCA
TTGTCGAGCGTCAAAAGAGCTTCTTCACGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACA
CTGGGTCTACACGAGTCCACGATCTCCCGTGTGACAACCAACAAATACATGGCGACCCCGATGGGGACGTTCGAGCTGAA
GTACTTCTTCGGCAGCCATGTATCGACGGAAACCGGCGGGGCGGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAAC
TTGTAGGAGCCGAAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAACTGTTAGGCGAACAAGGATTCGTGGTG
GCCCGTCGGACGGTGGCCAAATACCGCGAAGCCCTCAAGATCCCTGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-330)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-501)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

92.644

100

0.926