Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N4T19_RS13810 Genome accession   NZ_CP104377
Coordinates   2983101..2984717 (-) Length   538 a.a.
NCBI ID   WP_260718294.1    Uniprot ID   -
Organism   Comamonas sp. PR12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2978101..2989717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4T19_RS13785 (N4T19_13785) - 2978707..2979024 (-) 318 WP_027010449.1 DMT family transporter -
  N4T19_RS13790 (N4T19_13790) - 2979245..2979739 (-) 495 WP_116926257.1 putative toxin-antitoxin system toxin component, PIN family -
  N4T19_RS13795 (N4T19_13795) - 2979736..2981013 (-) 1278 WP_260718292.1 class I SAM-dependent RNA methyltransferase -
  N4T19_RS13800 (N4T19_13800) - 2981211..2981963 (+) 753 WP_260718293.1 hypothetical protein -
  N4T19_RS13805 (N4T19_13805) - 2982111..2983025 (+) 915 WP_409515661.1 aspartyl/asparaginyl beta-hydroxylase domain-containing protein -
  N4T19_RS13810 (N4T19_13810) rpoN/rpoN1 2983101..2984717 (-) 1617 WP_260718294.1 RNA polymerase factor sigma-54 Machinery gene
  N4T19_RS13815 (N4T19_13815) lptB 2984823..2985599 (-) 777 WP_116926389.1 LPS export ABC transporter ATP-binding protein -
  N4T19_RS13820 (N4T19_13820) lptA 2985623..2986291 (-) 669 WP_260718295.1 lipopolysaccharide transport periplasmic protein LptA -
  N4T19_RS13825 (N4T19_13825) - 2986420..2987795 (+) 1376 WP_260718296.1 IS3 family transposase -
  N4T19_RS13830 (N4T19_13830) - 2987865..2988509 (-) 645 WP_021025053.1 thiol:disulfide interchange protein DsbA/DsbL -
  N4T19_RS13835 (N4T19_13835) - 2988566..2989270 (-) 705 WP_116926251.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 538 a.a.        Molecular weight: 59045.14 Da        Isoelectric Point: 4.4515

>NTDB_id=729222 N4T19_RS13810 WP_260718294.1 2983101..2984717(-) (rpoN/rpoN1) [Comamonas sp. PR12]
MMPSLSLRVSQNLTLTPQLQQSIRLLQLSTQELTQEVEQMLDENPFLEREGDEAPEAEARKEAAESAEAADAADAADKGA
DTAASTDTNNSSDFEPGTSDQPLDFAAGKETGVDAEAAAGDSASDWEEWGGDLPARSSASDDDDETPMSDRRSEHLSLQD
VLHRQALSLRLCQEDRAALYFLIESLNDDGYLEETLAELAQSFGDASDPERYEALLHHLTVALRLLQSLEPIGVGARNLS
ECMQLQIKAQQRLLDEEGSDTPEADALALETALLVCQESLELLARRDVRKLVQLTGCSDAAVRSALALIAKLEPRPGRPY
ADVDRNTIIPDVLVRPSNERGLPVPGFSVQLNAEVMPRLRVQDVYANALRSHKGSEGHGALQQRLQEARWFIKNIQQRFD
TILRVSQAIVERQRNFFVHGELAMRPMVLREIADELGLHESTISRVTTAKYMATPQGTFELKYFFGSGLGTETGGSASST
AVKALIKQLLDEESAKKPLSDAKIADLLQAQGIECARRTVAKYREAMRIPPASLRKAL

Nucleotide


Download         Length: 1617 bp        

>NTDB_id=729222 N4T19_RS13810 WP_260718294.1 2983101..2984717(-) (rpoN/rpoN1) [Comamonas sp. PR12]
ATGATGCCGTCGCTGTCGCTGCGCGTATCGCAGAACCTGACCCTGACCCCCCAGCTGCAGCAGTCGATTCGCCTGCTGCA
GCTGTCCACGCAGGAGCTGACACAGGAAGTCGAGCAGATGCTCGACGAAAACCCGTTTCTGGAGCGCGAGGGCGATGAGG
CCCCCGAGGCTGAAGCCCGTAAAGAAGCCGCTGAATCTGCCGAAGCTGCAGACGCTGCAGACGCAGCCGACAAGGGTGCC
GATACTGCTGCCAGCACCGACACCAACAACAGTAGCGATTTCGAGCCCGGAACCTCCGACCAGCCGCTGGATTTTGCTGC
GGGCAAGGAAACCGGCGTCGATGCCGAGGCCGCTGCCGGCGATTCGGCATCGGACTGGGAAGAGTGGGGCGGCGATCTGC
CCGCCCGCTCCAGCGCCAGCGATGACGACGACGAGACGCCGATGTCCGACCGGCGCAGCGAGCACCTGAGCCTGCAGGAT
GTGCTGCACCGCCAGGCCTTGTCGCTGCGCCTGTGCCAGGAAGACCGGGCCGCGCTCTACTTTCTGATTGAATCACTGAA
CGACGACGGCTACCTCGAAGAAACGCTGGCCGAGCTGGCGCAAAGCTTTGGCGATGCCTCGGATCCCGAACGCTACGAAG
CCCTGCTGCACCACCTGACCGTGGCGCTGCGCTTGCTGCAAAGTCTGGAGCCGATTGGCGTGGGCGCGCGCAACCTGTCC
GAATGCATGCAGCTGCAGATCAAGGCCCAGCAGCGGCTGTTGGATGAAGAGGGCTCGGACACGCCCGAGGCCGATGCGCT
GGCGCTGGAGACCGCTTTGCTGGTCTGCCAGGAATCGCTGGAGCTGCTGGCGCGCCGTGATGTGCGCAAGCTGGTGCAAC
TGACCGGATGCAGTGATGCCGCGGTGCGCAGCGCGCTGGCGCTGATCGCCAAGCTCGAGCCCCGCCCCGGCCGGCCCTAT
GCCGATGTGGACCGCAACACCATCATCCCCGATGTCCTCGTGCGACCCAGCAATGAGCGCGGCCTGCCGGTGCCCGGTTT
CAGCGTGCAGCTCAATGCCGAGGTGATGCCGCGCCTGCGCGTGCAGGATGTCTACGCCAATGCCTTGCGCAGCCACAAGG
GCAGCGAGGGCCATGGCGCGCTGCAGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTTGAC
ACCATCTTGCGCGTCTCGCAGGCCATCGTCGAGCGCCAGCGCAATTTCTTTGTCCATGGCGAGCTGGCGATGCGCCCCAT
GGTGCTGCGCGAGATTGCCGATGAGCTGGGCCTGCACGAGTCCACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCA
CGCCGCAGGGCACGTTCGAGCTCAAGTACTTCTTTGGCTCGGGCCTGGGCACCGAGACCGGCGGCAGTGCGTCGAGCACG
GCGGTCAAGGCGCTGATCAAGCAGCTGCTGGACGAGGAAAGCGCGAAAAAGCCGCTGTCGGATGCAAAAATCGCCGATCT
GCTGCAGGCCCAGGGCATTGAATGCGCGCGCCGCACGGTGGCCAAGTACCGCGAGGCCATGCGCATCCCGCCGGCCAGCC
TGCGCAAGGCGCTGTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(381-536)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(151-365)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.365

100

0.513