Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   N4T35_RS18225 Genome accession   NZ_CP104342
Coordinates   3675175..3677403 (+) Length   742 a.a.
NCBI ID   WP_000156642.1    Uniprot ID   A0A427R1W7
Organism   Acinetobacter baumannii strain 2021CK-01300     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3670175..3682403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4T35_RS18200 (N4T35_18200) lnt 3670561..3672120 (+) 1560 WP_001203084.1 apolipoprotein N-acyltransferase -
  N4T35_RS18205 (N4T35_18205) - 3672122..3672460 (-) 339 WP_000434938.1 tRNA-binding protein -
  N4T35_RS18210 (N4T35_18210) - 3672605..3672949 (-) 345 WP_000643174.1 hypothetical protein -
  N4T35_RS18215 (N4T35_18215) gspG 3673216..3673797 (-) 582 WP_000865310.1 type II secretion system major pseudopilin GspG -
  N4T35_RS18220 (N4T35_18220) gspF 3673821..3675026 (-) 1206 WP_001112089.1 type II secretion system inner membrane protein GspF -
  N4T35_RS18225 (N4T35_18225) priA 3675175..3677403 (+) 2229 WP_000156642.1 primosomal protein N' Machinery gene
  N4T35_RS18230 (N4T35_18230) - 3677480..3679321 (+) 1842 WP_000008979.1 monovalent cation:proton antiporter-2 (CPA2) family protein -
  N4T35_RS18235 (N4T35_18235) - 3679487..3680362 (+) 876 WP_001281765.1 Hsp33 family molecular chaperone HslO -
  N4T35_RS18240 (N4T35_18240) - 3680500..3681111 (+) 612 WP_001105729.1 LysE family translocator -
  N4T35_RS18245 (N4T35_18245) - 3681262..3682218 (+) 957 WP_001107919.1 DnaJ C-terminal domain-containing protein -

Sequence

Protein


Download         Length: 742 a.a.        Molecular weight: 83941.78 Da        Isoelectric Point: 8.5662

>NTDB_id=728836 N4T35_RS18225 WP_000156642.1 3675175..3677403(+) (priA) [Acinetobacter baumannii strain 2021CK-01300]
MTITPKPDSVVYRVRVAVPVHLYDTFDYTLTKAQYERAQVGSRVAISFGRQNLIGIITEKVDPQESFTGHFQLKAISELL
DDEPILDEQVLNLLTWSAQYYQFPIGEVMQTALPALLRQGKPMDVLFHLWKIIPCDNAEALLKRSIKQQDAYQILKLHPA
GTTENILNLSGVETATLKALQKKGLVDCTLEPHDFSPAPVQLTQMPLTLNEDQKKATQHVLNAQHQYQAFLLDGLTGSGK
TEVYLHIMHEVLKQGKQVLVLVPEIGLTPQTISRFKSRFNCDIALLHSGLNDSKRLQAWQQAQTGKASIILGTRSAIYTP
LPRLGLIILDEEHDLSYKQQEGFRYHARDVALYRGHLQGCPVLLGSATPSIDSYHLVETGKLTALQLNQRAGHALLPKMH
LIDLKIVKKQHGISQPLIEQIKNTLARKEQVLIFLNRRGYAPVLVCESCGWQANCPHCDAHFTLHTQPYSYLHCHHCGTV
HRLPDHCPECQQKSLKTLGAGTAKVEEHLQELFPDHDVIRVDRDSTSRVGSWQKIYDRIQQNKPSILLGTQMLAKGHHFP
HVTLVAILDIDAGLLSVDIRAPERTAQLIVQVAGRAGRGEHKGHVYLQTLRPDHPLLTTLIEKDYRAVAKQTLAERKVAL
LPPYRYAVLIRAESKDRDYTLHFLNEAAEQLRQIAGDIVDIWGPIPAPMERKAGRYRAHMVILSADRARLHFYLRQWWAQ
LVHAPRQHQLRLSIDVDPQEFS

Nucleotide


Download         Length: 2229 bp        

>NTDB_id=728836 N4T35_RS18225 WP_000156642.1 3675175..3677403(+) (priA) [Acinetobacter baumannii strain 2021CK-01300]
ATGACCATAACGCCAAAGCCTGATTCAGTTGTTTATCGTGTGCGTGTTGCCGTACCTGTGCATCTGTACGACACTTTTGA
CTATACACTCACCAAAGCACAATATGAACGGGCTCAAGTAGGTTCACGCGTCGCGATTTCATTTGGACGGCAAAACCTGA
TTGGTATCATTACTGAAAAAGTTGACCCACAAGAATCATTTACAGGTCACTTTCAGCTTAAGGCCATTTCTGAACTTTTA
GATGATGAGCCTATTTTAGATGAACAAGTTTTAAATTTATTGACATGGTCAGCACAATATTACCAATTTCCGATTGGCGA
GGTGATGCAGACTGCTTTACCTGCGTTATTGCGTCAGGGCAAACCCATGGATGTTTTATTTCATCTATGGAAAATCATTC
CATGTGATAATGCCGAAGCTTTATTAAAACGCTCAATCAAACAGCAAGACGCTTATCAAATTTTAAAATTACATCCTGCC
GGAACCACTGAAAATATTCTCAATTTAAGTGGTGTAGAAACTGCAACACTTAAAGCACTGCAAAAGAAAGGTCTCGTAGA
CTGTACGCTTGAGCCTCATGACTTTAGCCCAGCCCCTGTGCAATTGACGCAAATGCCACTCACGCTCAATGAAGACCAAA
AGAAGGCCACCCAGCACGTCCTTAATGCGCAGCATCAATATCAAGCGTTTTTGTTGGATGGTTTAACCGGAAGTGGTAAA
ACTGAAGTTTATTTGCACATCATGCATGAGGTGTTAAAGCAAGGTAAGCAAGTACTGGTGTTAGTTCCCGAAATTGGTTT
AACCCCTCAAACCATTTCGCGCTTTAAGTCTCGTTTTAACTGTGACATTGCTTTATTACATTCAGGCTTAAATGACTCTA
AACGCCTACAAGCCTGGCAGCAAGCACAGACTGGAAAGGCCTCTATTATTTTAGGAACACGTTCAGCCATTTATACCCCG
CTGCCACGTTTGGGCCTGATCATTTTAGATGAAGAACATGATCTTTCTTATAAACAACAGGAAGGTTTCCGTTACCATGC
GCGTGATGTCGCGCTTTATCGAGGACATTTACAAGGCTGTCCTGTTCTTTTAGGTTCGGCAACCCCAAGCATTGATAGCT
ATCACTTAGTCGAAACTGGCAAGTTAACTGCACTTCAGCTGAACCAGCGTGCAGGCCATGCACTTTTACCAAAAATGCAT
TTGATTGATCTTAAAATTGTCAAAAAACAGCATGGTATTAGTCAGCCTTTAATTGAACAGATTAAAAATACCCTAGCAAG
AAAAGAACAAGTTTTAATTTTTTTAAACCGACGTGGTTATGCACCTGTTCTGGTCTGTGAAAGTTGTGGTTGGCAAGCGA
ATTGTCCGCATTGTGATGCGCACTTTACGTTACACACTCAGCCCTACTCTTACTTACATTGTCACCACTGTGGCACCGTT
CATCGCCTGCCAGACCACTGCCCTGAATGTCAGCAAAAGAGCCTCAAAACTTTAGGTGCTGGCACAGCCAAGGTTGAAGA
GCATTTACAAGAATTATTCCCAGATCATGATGTGATTCGGGTCGATCGTGACAGCACCAGCCGAGTCGGTAGCTGGCAAA
AAATTTATGATCGCATTCAACAAAATAAACCTTCGATTTTGCTCGGTACGCAAATGTTGGCAAAGGGTCACCATTTTCCG
CATGTGACTCTGGTTGCTATTTTAGACATCGATGCAGGTTTATTGAGTGTCGATATTCGGGCGCCAGAACGTACTGCTCA
ATTGATTGTACAAGTGGCTGGTCGTGCAGGTCGTGGAGAACACAAAGGTCATGTTTATTTACAAACATTAAGGCCAGATC
ATCCTTTACTCACCACACTCATCGAAAAAGATTATCGGGCAGTCGCGAAACAAACATTGGCTGAGCGTAAAGTTGCATTA
CTGCCGCCTTATCGCTATGCCGTACTTATACGCGCTGAATCGAAAGATCGTGACTATACCTTGCATTTTTTAAATGAAGC
TGCCGAGCAACTTCGCCAAATTGCAGGTGACATTGTTGATATCTGGGGGCCAATTCCAGCGCCAATGGAACGTAAAGCGG
GACGTTACCGCGCACATATGGTGATTTTGTCTGCTGACCGCGCTCGTCTGCATTTTTACTTAAGACAATGGTGGGCTCAG
TTGGTCCATGCGCCAAGACAGCATCAACTTAGACTCTCAATTGATGTTGACCCGCAAGAATTTAGTTAG

Domains

Predicted by InterproScan.

Primosomal protein N C-terminal domain (PriA_C)

(643-738)

Type III restriction enzyme, res subunit (ResIII)

(208-369)

3DNA-binding domain (3BD) (PriA_3primeBD)

(15-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A427R1W7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

97.978

100

0.98