Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   N4J17_RS14435 Genome accession   NZ_CP104311
Coordinates   2967576..2968952 (-) Length   458 a.a.
NCBI ID   WP_232470414.1    Uniprot ID   -
Organism   Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2962576..2973952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4J17_RS14415 (N4J17_14425) - 2963224..2964714 (+) 1491 WP_198322650.1 oligosaccharide flippase family protein -
  N4J17_RS14420 (N4J17_14430) - 2965031..2965459 (+) 429 WP_198322649.1 universal stress protein -
  N4J17_RS14425 (N4J17_14435) - 2965456..2966328 (+) 873 WP_232470400.1 cytochrome c family protein -
  N4J17_RS14430 (N4J17_14440) - 2966376..2967428 (-) 1053 WP_232470399.1 integrase -
  N4J17_RS14435 (N4J17_14445) radA 2967576..2968952 (-) 1377 WP_232470414.1 DNA repair protein RadA Machinery gene
  N4J17_RS14440 (N4J17_14450) - 2968949..2969692 (-) 744 WP_198322647.1 class I SAM-dependent methyltransferase -
  N4J17_RS14445 (N4J17_14455) htpX 2969800..2970684 (+) 885 WP_198322646.1 protease HtpX -
  N4J17_RS14450 (N4J17_14460) - 2970729..2971532 (-) 804 WP_198322645.1 sulfite exporter TauE/SafE family protein -
  N4J17_RS14455 (N4J17_14465) - 2971630..2972058 (-) 429 WP_198322644.1 TOBE domain-containing protein -
  N4J17_RS14460 (N4J17_14470) modC 2972187..2973257 (-) 1071 WP_198322673.1 molybdenum ABC transporter ATP-binding protein -
  N4J17_RS14465 (N4J17_14475) modB 2973260..2973937 (-) 678 WP_198322643.1 molybdate ABC transporter permease subunit -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 48667.63 Da        Isoelectric Point: 7.7342

>NTDB_id=728579 N4J17_RS14435 WP_232470414.1 2967576..2968952(-) (radA) [Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)]
MSAARGSKPSRVVYRCTGCGQSQPKWSGQCGGCGQWNTLVEEIEAPAVGRRQVGYAGVAEEGTPVSLTEVSREELSRVTS
GLGELDRVLGGGVVPGSAILLGGDPGIGKSTLLLQTLAYLGKSARTLYVTGEESLSQVSLRAHRLGLDGHGIQALAETSL
ERILDTAGRHKPEVLVVDSIQTVYSEALQSAPGSVGQVRECAAQLVRYAKQSSTTVFLVGHVTKEGALAGPRVLEHMVDT
VLYFEGDASSRYRVVRSFKNRFGAVNELGVFAMTETGLKEVRNPSAIFLSRGDEDMPGSIVTVTREGSRPLLVEVQALVD
ESHLQAPRRLVVGMEMNRLAMLLAVLHRHGGVPVAAQDVFVNLVGGVRLTETAGDLAVACAVVSSFRDRPVPGDWVIFGE
LGLNGEIRPVPNGEERLREAAKHGFKYALVPAKNAPKHGVSGISIIPAKNLREAIGSL

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=728579 N4J17_RS14435 WP_232470414.1 2967576..2968952(-) (radA) [Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)]
CTGAGCGCTGCCAGAGGGTCCAAGCCCAGCAGGGTGGTCTACCGCTGCACTGGGTGTGGCCAGAGTCAGCCGAAATGGTC
CGGTCAGTGCGGTGGCTGTGGACAGTGGAACACCCTGGTCGAGGAAATCGAAGCGCCGGCGGTCGGCCGGCGTCAGGTCG
GCTACGCCGGCGTGGCGGAGGAAGGCACGCCAGTGTCATTGACCGAGGTGTCCCGCGAGGAGCTCTCGCGCGTCACCAGC
GGCCTGGGCGAACTCGACCGGGTGCTCGGTGGCGGGGTGGTGCCGGGTTCGGCGATTCTGCTCGGCGGCGATCCGGGTAT
CGGCAAATCCACCTTGCTGCTGCAGACCCTGGCCTATCTGGGCAAGAGTGCCCGCACACTGTATGTGACCGGTGAGGAAT
CGCTCAGCCAGGTCAGTCTCCGGGCTCATCGCCTGGGACTGGACGGCCACGGCATTCAGGCATTGGCCGAGACCTCACTG
GAACGCATCCTCGACACCGCGGGGCGGCACAAGCCAGAGGTGCTGGTGGTCGATTCGATCCAGACCGTATACAGCGAAGC
CTTGCAGTCCGCCCCCGGTTCAGTGGGGCAGGTGCGGGAATGCGCGGCGCAACTGGTGCGTTATGCCAAGCAGAGTTCGA
CCACCGTGTTCCTGGTCGGCCACGTCACCAAGGAGGGGGCGCTGGCCGGTCCCCGGGTGCTGGAGCACATGGTCGATACC
GTGCTGTATTTCGAGGGCGATGCGTCCAGCCGCTACCGGGTGGTGCGCTCGTTCAAGAACCGCTTCGGCGCGGTCAACGA
ACTGGGCGTCTTCGCCATGACGGAGACCGGTCTCAAGGAAGTACGCAACCCTTCGGCCATATTCCTGTCGCGGGGTGACG
AAGACATGCCGGGCAGCATCGTGACCGTGACCCGCGAGGGCAGCCGGCCTTTGCTGGTCGAAGTCCAGGCACTGGTTGAC
GAATCCCATCTGCAGGCCCCGCGACGGCTGGTGGTGGGCATGGAAATGAACCGGCTGGCGATGCTGCTGGCCGTGTTGCA
CCGCCACGGAGGCGTGCCGGTCGCAGCGCAGGACGTGTTCGTCAACCTGGTCGGCGGCGTTCGCCTTACCGAGACCGCCG
GCGACCTCGCCGTGGCCTGTGCCGTGGTCTCCAGTTTCCGCGACCGCCCCGTGCCCGGCGATTGGGTCATCTTCGGCGAA
CTCGGTCTCAATGGCGAAATTCGCCCCGTCCCCAACGGCGAAGAGCGACTGCGCGAAGCCGCCAAGCACGGCTTCAAGTA
CGCCCTGGTGCCGGCCAAAAACGCGCCGAAGCACGGTGTGTCGGGCATTTCCATCATCCCCGCGAAAAATCTGCGGGAAG
CGATCGGCTCGCTTTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(14-41)

Subunit ChlI of Mg-chelatase (ChlI)

(350-434)

AAA domain (AAA_25)

(87-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.667

98.472

0.489
  radA Streptococcus mitis NCTC 12261

45.701

96.507

0.441
  radA Streptococcus mitis SK321

46.083

94.76

0.437
  radA Streptococcus pneumoniae TIGR4

45.977

94.978

0.437
  radA Streptococcus pneumoniae D39

45.977

94.978

0.437
  radA Streptococcus pneumoniae Rx1

45.977

94.978

0.437
  radA Streptococcus pneumoniae R6

45.977

94.978

0.437