Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   N4T21_RS12595 Genome accession   NZ_CP104303
Coordinates   2561622..2562986 (-) Length   454 a.a.
NCBI ID   WP_003567254.1    Uniprot ID   A0A829GUQ3
Organism   Lacticaseibacillus paracasei strain VHProbi O44     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2556622..2567986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4T21_RS12575 (N4T21_12575) - 2557119..2558135 (-) 1017 WP_260642910.1 DUF5808 domain-containing protein -
  N4T21_RS12580 (N4T21_12580) - 2558142..2558516 (-) 375 WP_016388389.1 GntR family transcriptional regulator -
  N4T21_RS12585 (N4T21_12585) - 2558681..2559952 (-) 1272 WP_260642911.1 MucBP domain-containing protein -
  N4T21_RS16405 - 2560277..2560372 (+) 96 Protein_2469 MutR family positive transcriptional regulator -
  N4T21_RS12590 (N4T21_12590) - 2560485..2561600 (-) 1116 WP_003567252.1 PIN/TRAM domain-containing protein -
  N4T21_RS12595 (N4T21_12595) radA 2561622..2562986 (-) 1365 WP_003567254.1 DNA repair protein RadA Machinery gene
  N4T21_RS12600 (N4T21_12600) - 2563003..2563545 (-) 543 WP_003567256.1 dUTP diphosphatase -
  N4T21_RS12605 (N4T21_12605) - 2563766..2564056 (+) 291 WP_003567258.1 GNAT family N-acetyltransferase -
  N4T21_RS12610 (N4T21_12610) - 2564172..2564549 (-) 378 WP_003567260.1 DUF805 domain-containing protein -
  N4T21_RS12615 (N4T21_12615) - 2564794..2566113 (+) 1320 WP_003571381.1 C1 family peptidase -
  N4T21_RS12620 (N4T21_12620) - 2566347..2567693 (+) 1347 WP_260642912.1 aminopeptidase C -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49126.47 Da        Isoelectric Point: 7.4216

>NTDB_id=728523 N4T21_RS12595 WP_003567254.1 2561622..2562986(-) (radA) [Lacticaseibacillus paracasei strain VHProbi O44]
MAKAKTQYVCQNCGYISATYLGRCPNCGGWNTLVEETVSSSKSVPRQTAAGSKVKPTRMNDVTITKETRVKTGLDELNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLANTGGTVLYVSGEESASQIKMRAGRLGVANSGMYLYPETDMPSIEDVI
NQMQPDYVVIDSVQTMNVPEMNSAVGSVAQIREVTAELMRIAKSKGVTIFIVGHVTKEGAIAGPKILEHMVDTVLYFEGD
MHHTYRILRSVKNRFGSTNEIGIFEMHQDGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVEIQALISPTMYGNA
KRTSSGLDHNRVSLIMAVLEKRANLMLQNQDAYLKATGGVKLDEPAIDLAMAVAIASSYRDKEISPTDCFVGEIGLTGEV
RRVNRIEERVKEAAKLGFKRIFVPRNNLQGWHAPKDIQVIGVTSIAEALHKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=728523 N4T21_RS12595 WP_003567254.1 2561622..2562986(-) (radA) [Lacticaseibacillus paracasei strain VHProbi O44]
ATGGCGAAAGCAAAAACACAATATGTTTGCCAAAACTGTGGCTATATTTCAGCCACTTATCTAGGCCGGTGTCCAAATTG
CGGCGGCTGGAACACCTTAGTGGAAGAAACCGTCAGTTCAAGCAAGTCTGTGCCGCGACAAACAGCAGCCGGCAGTAAGG
TAAAGCCGACGCGGATGAACGATGTGACGATCACCAAAGAAACGCGCGTCAAGACTGGCTTAGATGAACTGAACCGGGTG
CTTGGCGGCGGAGTGGTGCCAGGGTCACTGGTGCTGATCGGCGGGGATCCGGGGATTGGTAAATCAACCTTGCTATTACA
AGTATCAGGGCAGTTGGCTAATACCGGCGGAACCGTTTTGTATGTCTCTGGCGAAGAAAGTGCCAGTCAGATCAAAATGC
GGGCTGGCCGGCTTGGCGTGGCAAATTCGGGGATGTACTTGTATCCCGAAACCGACATGCCGAGTATTGAAGACGTCATC
AACCAAATGCAGCCGGATTACGTTGTGATCGACTCCGTTCAAACTATGAATGTGCCAGAAATGAATTCAGCGGTCGGTTC
TGTGGCACAGATTCGCGAAGTGACGGCAGAACTGATGCGCATTGCCAAGTCCAAAGGCGTGACCATTTTCATCGTTGGCC
ATGTCACTAAGGAAGGGGCGATTGCCGGGCCGAAGATTCTTGAGCATATGGTCGACACGGTGCTCTATTTTGAAGGCGAC
ATGCACCACACGTATCGAATCTTAAGATCCGTCAAAAATCGATTTGGATCGACCAATGAAATCGGCATTTTCGAAATGCA
TCAAGACGGGTTACAGGAAGTCGCCAATCCCTCGGAAATCTTCTTGGAAGAACGCTTGGCGGGGGCAACCGGTTCTGCGG
TGGTTGTTTCGATGGAAGGCACCCGACCAATCTTAGTCGAGATTCAAGCCTTGATCAGTCCAACGATGTATGGCAATGCT
AAACGGACGAGCAGTGGGTTGGATCACAATCGCGTGAGCTTGATCATGGCCGTTCTTGAGAAGCGGGCTAACTTGATGTT
GCAAAATCAGGACGCCTATCTCAAGGCAACAGGCGGTGTGAAACTCGATGAGCCGGCGATTGACCTCGCCATGGCTGTGG
CAATTGCGTCATCTTATCGAGATAAGGAGATTTCACCGACAGATTGTTTTGTCGGTGAAATCGGATTGACGGGGGAAGTG
CGCCGCGTCAATCGGATCGAAGAACGTGTCAAAGAGGCGGCCAAATTGGGTTTCAAACGGATTTTTGTACCCCGTAACAA
CTTGCAAGGCTGGCATGCGCCAAAAGATATTCAAGTCATTGGTGTTACAAGCATTGCCGAAGCGCTGCATAAGGTCTTTA
ATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(69-219)

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A829GUQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.212

99.78

0.681
  radA Streptococcus pneumoniae Rx1

68.212

99.78

0.681
  radA Streptococcus pneumoniae D39

68.212

99.78

0.681
  radA Streptococcus pneumoniae R6

68.212

99.78

0.681
  radA Streptococcus pneumoniae TIGR4

68.212

99.78

0.681
  radA Streptococcus mitis SK321

67.991

99.78

0.678
  radA Bacillus subtilis subsp. subtilis str. 168

64.758

100

0.648