Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   W01_RS09650 Genome accession   NZ_AP019547
Coordinates   2151632..2153059 (+) Length   475 a.a.
NCBI ID   WP_173054196.1    Uniprot ID   A0A455XER5
Organism   Candidatus Nitrotoga sp. AM1P     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2146632..2158059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  W01_RS09620 (W01_18770) hisD 2146826..2148124 (+) 1299 WP_173054184.1 histidinol dehydrogenase -
  W01_RS09625 (W01_18780) - 2148224..2149210 (+) 987 WP_173054186.1 KpsF/GutQ family sugar-phosphate isomerase -
  W01_RS09630 (W01_18790) - 2149210..2149728 (+) 519 WP_173054188.1 HAD hydrolase family protein -
  W01_RS09635 (W01_18800) lptC 2149725..2150321 (+) 597 WP_173054190.1 LPS export ABC transporter periplasmic protein LptC -
  W01_RS09640 (W01_18810) lptA 2150359..2150874 (+) 516 WP_242006931.1 lipopolysaccharide transport periplasmic protein LptA -
  W01_RS09645 (W01_18820) lptB 2150871..2151593 (+) 723 WP_173054194.1 LPS export ABC transporter ATP-binding protein -
  W01_RS09650 (W01_18830) rpoN/rpoN1 2151632..2153059 (+) 1428 WP_173054196.1 RNA polymerase factor sigma-54 Machinery gene
  W01_RS09655 (W01_18840) raiA 2153076..2153405 (+) 330 WP_173054198.1 ribosome-associated translation inhibitor RaiA -
  W01_RS09660 (W01_18850) - 2153457..2153912 (+) 456 WP_173054200.1 PTS sugar transporter subunit IIA -
  W01_RS09665 (W01_18860) hprK 2153918..2154856 (+) 939 WP_173054202.1 HPr(Ser) kinase/phosphatase -
  W01_RS09670 (W01_18870) rapZ 2154860..2155729 (+) 870 WP_173054204.1 RNase adapter RapZ -
  W01_RS09675 (W01_18880) - 2156150..2156749 (+) 600 WP_173054206.1 flavin prenyltransferase UbiX -
  W01_RS09680 (W01_18890) - 2157005..2157199 (+) 195 WP_173054208.1 hypothetical protein -
  W01_RS09685 (W01_18900) - 2157202..2157516 (+) 315 WP_173054210.1 cell division protein ZapA -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 53937.35 Da        Isoelectric Point: 4.8795

>NTDB_id=72851 W01_RS09650 WP_173054196.1 2151632..2153059(+) (rpoN/rpoN1) [Candidatus Nitrotoga sp. AM1P]
MKPTLQLRQSQQLTLTPQLQQAIRLLQLSTLEVNQEVARLLDENPLLERADEDAMQPYAHGDAASSAVVDNTEVKDEMPQ
QTDEDWVKEDWNEPSFSAASGPDDEKEARAEVAADTPSLREHLIWQLNMSQHDVRDRKIIGLLIDALDGNGYLAQPLEEI
VEMLPEQLDITLDDLETALVQLQHLDQPGIAARTLSECLTLQLKALPEDTPCRDLALRLVSQYLNLLGAHDFTKLKKVLR
CDDDALRTVQSLITHLQPRPGAEFEQRAADYVVPDVVVEFRHGNWHVRLNQGAMPRLRVNQMYANILQQGNEKNVQQLAG
QLQEARWFIKNLQQRFDTILRVSQTIVDRQRNFLEHGDTAMCPLVLREIAEQLDLHESTISRVTTQKFMLTPRGIYELKY
FFCSGIATESGGTCSSTAIRALIKQLVNQEDARHPLTDSRMSEILAQQGILVARRTVAKYRELLNILPVNLRKSL

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=72851 W01_RS09650 WP_173054196.1 2151632..2153059(+) (rpoN/rpoN1) [Candidatus Nitrotoga sp. AM1P]
ATGAAACCCACATTACAGCTTAGGCAATCCCAGCAACTGACGCTTACCCCCCAGTTACAGCAAGCCATACGCCTGTTGCA
ACTATCCACTCTGGAAGTCAATCAGGAAGTAGCACGTTTGCTGGATGAAAATCCATTGTTAGAACGTGCAGATGAAGATG
CGATGCAGCCCTATGCGCATGGAGATGCTGCTTCTAGCGCTGTTGTAGATAATACTGAAGTAAAAGATGAAATGCCGCAA
CAGACCGATGAAGACTGGGTAAAAGAAGACTGGAATGAACCTTCTTTTTCCGCAGCCAGCGGGCCGGATGATGAAAAAGA
AGCTCGTGCGGAAGTCGCGGCAGATACCCCAAGCCTGCGTGAGCACCTCATCTGGCAACTAAATATGAGTCAGCATGATG
TGCGCGACCGAAAAATTATCGGTTTGTTGATCGATGCATTGGACGGCAATGGTTATCTGGCGCAGCCGCTGGAAGAAATT
GTCGAAATGCTACCAGAACAACTTGATATTACTTTGGATGACTTGGAAACTGCACTGGTGCAATTACAGCACCTGGATCA
GCCCGGCATAGCTGCCCGTACCTTAAGCGAATGTCTCACGCTCCAACTTAAAGCGCTCCCGGAAGATACTCCATGCCGGG
ATCTGGCGCTACGCTTGGTGTCGCAATACCTCAACCTGCTGGGTGCGCACGATTTTACCAAGCTGAAAAAAGTCCTTCGC
TGTGACGATGACGCATTGCGCACCGTACAATCGCTCATTACTCATCTGCAACCAAGACCTGGCGCTGAATTTGAACAGCG
AGCCGCAGACTATGTCGTACCTGATGTGGTGGTAGAATTCCGCCACGGTAACTGGCATGTACGGCTAAATCAGGGCGCTA
TGCCGCGCCTGCGTGTTAATCAAATGTATGCCAATATCTTGCAACAGGGTAATGAAAAAAACGTACAGCAGCTGGCTGGT
CAATTGCAGGAAGCGCGCTGGTTTATCAAAAATTTGCAACAGCGTTTCGATACTATTTTGCGTGTATCGCAGACAATTGT
TGATCGTCAACGCAATTTTTTAGAGCATGGTGATACAGCGATGTGCCCGCTGGTACTGCGCGAAATTGCAGAACAGCTTG
ATCTGCATGAATCCACCATTTCCCGTGTGACTACACAAAAATTCATGCTTACCCCGCGCGGTATTTATGAACTCAAGTAT
TTTTTCTGCAGTGGAATCGCGACCGAAAGTGGCGGTACGTGTTCCTCCACGGCAATCCGCGCACTCATCAAGCAGCTTGT
CAACCAGGAAGACGCACGTCACCCTCTAACCGACAGCCGCATGTCTGAAATCCTGGCACAGCAGGGCATATTGGTTGCCC
GGCGTACCGTGGCAAAATACCGGGAATTGCTGAATATCCTCCCAGTTAATCTTCGTAAATCACTCTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(111-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(318-473)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A455XER5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.895

100

0.539

Multiple sequence alignment