Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   W01_RS03685 Genome accession   NZ_AP019547
Coordinates   819697..821055 (-) Length   452 a.a.
NCBI ID   WP_173052249.1    Uniprot ID   A0A455X9S8
Organism   Candidatus Nitrotoga sp. AM1P     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 814697..826055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  W01_RS03660 (W01_07070) - 814848..815144 (-) 297 WP_173052245.1 winged helix-turn-helix domain-containing protein -
  W01_RS14360 - 815123..815248 (-) 126 WP_256380110.1 hypothetical protein -
  W01_RS03665 (W01_07080) - 815441..815719 (+) 279 WP_173052246.1 type II toxin-antitoxin system RelE/ParE family toxin -
  W01_RS03670 (W01_07090) - 815731..816036 (+) 306 WP_173052247.1 HigA family addiction module antitoxin -
  W01_RS03675 (W01_07100) - 816309..817097 (-) 789 WP_173055713.1 IS3 family transposase -
  W01_RS03680 (W01_07110) - 817378..819546 (+) 2169 WP_173052248.1 hypothetical protein -
  W01_RS03685 (W01_07120) radA 819697..821055 (-) 1359 WP_173052249.1 DNA repair protein RadA Machinery gene
  W01_RS03690 (W01_07130) - 821454..822326 (-) 873 WP_173052250.1 tetratricopeptide repeat protein -
  W01_RS03695 (W01_07140) - 822562..823506 (-) 945 WP_173052251.1 rhodanese-related sulfurtransferase -
  W01_RS03700 (W01_07150) - 823739..824311 (-) 573 WP_173052252.1 response regulator transcription factor -
  W01_RS03705 (W01_07160) - 824308..825576 (-) 1269 WP_173052253.1 ATP-binding protein -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 48606.33 Da        Isoelectric Point: 7.7508

>NTDB_id=72839 W01_RS03685 WP_173052249.1 819697..821055(-) (radA) [Candidatus Nitrotoga sp. AM1P]
MAKAKIIYSCTECGGQVSKWQGQCPHCTAWNTLVESVAELATSGKNRYSSLATTSMMQTLAEVGAEEVPRTPTGIAEFDR
VLGGGLVSGGVVLIGGDPGIGKSTLLLQALAHLATHKKVLYVSGEESAQQIALRARRLSLDARGMYLLAEIQLEKIQSVI
QQEKPDVVVIDSIQTMYSEHLTSAPGSVAQVRESAAQLTRMAKSTAVTMILVGHVTKDGTLAGPRVLEHIVDTVLYFEGD
THSSFRMIRAFKNRFGAVNELGVFAMTEKGLREVSNPSALFLSQHGAQVAGSCVMVTQEGTRPLLVEIQALLDEAHSPNP
RRLSLGLEQNRLAMLLAVLHRHAGIACFDQDVFINAVGGVKITEPGADLPVLLAIVSSLRNKPLPEKLVVFGEVGLAGEI
RPVQRGQERLREAAKLGFTLAIIPKANKPKQEIAGMEIIAVERVEDAVAKMR

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=72839 W01_RS03685 WP_173052249.1 819697..821055(-) (radA) [Candidatus Nitrotoga sp. AM1P]
ATGGCGAAAGCAAAAATAATATATAGCTGCACTGAATGTGGCGGACAGGTTTCCAAATGGCAGGGGCAGTGTCCGCATTG
CACGGCTTGGAATACGCTGGTGGAAAGTGTGGCAGAACTGGCGACTAGCGGAAAAAATCGCTACAGCTCGCTGGCGACAA
CGAGCATGATGCAGACACTGGCGGAAGTAGGGGCGGAAGAAGTCCCGCGTACGCCAACTGGTATCGCCGAGTTTGATCGC
GTGTTGGGTGGCGGGTTAGTGAGTGGTGGTGTGGTTTTGATCGGCGGTGATCCCGGAATAGGTAAATCCACCCTGCTGTT
GCAAGCGCTGGCGCATCTCGCTACACATAAAAAAGTTTTATACGTCAGTGGCGAGGAATCGGCACAGCAAATTGCTTTGC
GTGCGAGGCGACTATCGCTGGATGCGCGGGGAATGTACCTGCTGGCTGAAATTCAGCTGGAAAAAATCCAGTCTGTCATC
CAGCAGGAAAAACCCGATGTGGTGGTGATCGATTCCATCCAGACCATGTATTCTGAACACCTCACATCAGCTCCCGGCTC
GGTAGCGCAAGTGCGTGAATCCGCAGCGCAACTCACGCGCATGGCGAAGAGCACAGCAGTGACCATGATTCTGGTAGGCC
ATGTTACTAAGGATGGGACGTTGGCCGGCCCGCGTGTGTTGGAACATATCGTAGATACAGTGCTGTATTTCGAGGGCGAT
ACCCACTCCAGCTTTCGCATGATACGCGCGTTTAAAAATCGCTTTGGTGCAGTAAACGAATTAGGCGTGTTCGCCATGAC
CGAAAAAGGATTGCGTGAGGTGAGCAACCCCTCCGCGTTGTTTTTGTCGCAGCATGGCGCGCAAGTGGCGGGTTCATGCG
TGATGGTGACGCAGGAAGGTACGCGTCCGCTGCTGGTGGAAATCCAGGCCCTGCTGGATGAGGCGCATTCGCCCAATCCG
CGTCGCCTTTCATTGGGTTTGGAACAAAATCGTTTGGCTATGTTGCTGGCCGTACTGCATCGTCATGCAGGCATCGCCTG
TTTTGATCAAGATGTGTTTATCAATGCGGTGGGTGGCGTGAAAATTACCGAGCCGGGTGCCGATTTGCCCGTGTTGCTGG
CTATCGTATCTTCGCTACGCAATAAACCTCTGCCAGAAAAATTGGTGGTGTTTGGAGAAGTCGGCTTGGCTGGCGAAATA
CGTCCGGTGCAGCGTGGTCAGGAACGCTTGCGAGAGGCTGCCAAGCTAGGCTTTACCTTAGCCATTATTCCCAAGGCTAA
TAAGCCGAAACAAGAAATAGCGGGTATGGAAATTATTGCGGTGGAGCGGGTGGAGGATGCAGTAGCGAAAATGCGTTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-427)

AAA domain (AAA_25)

(80-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A455X9S8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.127

100

0.498
  radA Streptococcus mitis NCTC 12261

47.368

100

0.478
  radA Streptococcus mitis SK321

47.368

100

0.478
  radA Streptococcus pneumoniae Rx1

47.046

100

0.476
  radA Streptococcus pneumoniae D39

47.046

100

0.476
  radA Streptococcus pneumoniae R6

47.046

100

0.476
  radA Streptococcus pneumoniae TIGR4

47.046

100

0.476

Multiple sequence alignment