Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N2K12_RS18075 Genome accession   NZ_CP104279
Coordinates   3808524..3810104 (-) Length   526 a.a.
NCBI ID   WP_260674397.1    Uniprot ID   -
Organism   Comamonas aquatica strain SCLZS63     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3803524..3815104
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N2K12_RS18060 - 3804577..3805065 (-) 489 WP_260674394.1 putative toxin-antitoxin system toxin component, PIN family -
  N2K12_RS18065 - 3805069..3806352 (-) 1284 WP_260674395.1 class I SAM-dependent RNA methyltransferase -
  N2K12_RS18070 - 3806505..3808331 (+) 1827 WP_260674396.1 methyl-accepting chemotaxis protein -
  N2K12_RS18075 rpoN/rpoN1 3808524..3810104 (-) 1581 WP_260674397.1 RNA polymerase factor sigma-54 Machinery gene
  N2K12_RS18080 lptB 3810155..3810925 (-) 771 WP_042417637.1 LPS export ABC transporter ATP-binding protein -
  N2K12_RS18085 lptA 3810925..3811563 (-) 639 WP_042417727.1 lipopolysaccharide transport periplasmic protein LptA -
  N2K12_RS18090 - 3811667..3812314 (-) 648 WP_042417634.1 thiol:disulfide interchange protein DsbA/DsbL -
  N2K12_RS18095 - 3812370..3813020 (-) 651 WP_042417631.1 SPOR domain-containing protein -
  N2K12_RS18100 argS 3813051..3814760 (-) 1710 WP_260674398.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 526 a.a.        Molecular weight: 57975.32 Da        Isoelectric Point: 4.8592

>NTDB_id=728217 N2K12_RS18075 WP_260674397.1 3808524..3810104(-) (rpoN/rpoN1) [Comamonas aquatica strain SCLZS63]
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDDNPFLELASDDAPREEFGLQAADEPAQHEGASADETPY
SIADSAGTASAEGQKDQESSAPDSDVPSDWEGDGSVDIAHDDSEWGGDAPARGNRNDDEDAPTALDLAGAHESLTDFLHQ
QALSLRLSEIDRYALRFLIENLNDDGYLEDPLQELAEGLAGTDDLEQLDELVHRFTVALRLLQSLEPVGVGARDLAECLR
LQLLHLHREDPQDEMLGVALQVCAQPLEMLARRDVRKLMQACGLPEAKVRAALGVIARLEPKPGRRFTDVERHIIVPDVI
VKKTARKGGDGLAQFSVQLNPDVMPRLRVHDVYANALRSHKGSDGHAGLQQRLQEARWFIKNVQQRFDTILRVSKAIVDR
QKNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPHGTYELKFFFGSGLGTENGGNASSTAVRALIKQFVAA
ESPKKPLSDSQIADMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1581 bp        

>NTDB_id=728217 N2K12_RS18075 WP_260674397.1 3808524..3810104(-) (rpoN/rpoN1) [Comamonas aquatica strain SCLZS63]
TTGAAGCCAGGTTTGTCACTGCGGGTCTCACAGCATCTGGCGCTCACGCCGCAGTTGCAGCAATCCATCCGGCTGTTGCA
GCTGTCCACCCTGGAGTTATCCCAGGAAGTCGAGCAGATGCTCGACGACAACCCGTTTCTGGAGCTGGCCTCGGACGACG
CCCCGCGCGAGGAGTTCGGCCTGCAGGCCGCGGACGAGCCCGCCCAGCACGAGGGCGCCTCGGCCGACGAAACACCATAT
TCCATAGCAGACAGCGCAGGCACAGCCAGCGCTGAAGGCCAAAAAGACCAGGAATCCTCGGCGCCCGACAGCGACGTGCC
CAGCGACTGGGAGGGCGACGGCAGCGTCGACATCGCGCACGACGACAGCGAATGGGGCGGCGACGCCCCGGCGCGCGGCA
ACCGCAATGACGACGAGGATGCCCCCACGGCCCTGGATCTGGCGGGCGCGCACGAGTCGCTGACCGATTTTCTGCACCAG
CAGGCGCTGTCGCTGCGCCTGTCCGAGATCGACCGCTATGCGCTGCGTTTCCTGATCGAAAACCTCAACGACGATGGCTA
CCTCGAGGACCCGCTGCAGGAGTTGGCCGAGGGCCTGGCCGGCACCGACGACCTGGAGCAGCTGGACGAGCTGGTCCACC
GCTTCACCGTGGCCCTGCGTCTGCTGCAGTCGCTGGAGCCCGTGGGCGTGGGCGCCCGCGACCTGGCCGAATGCCTGCGC
CTGCAACTGCTGCACCTGCACCGCGAGGACCCGCAGGACGAGATGCTGGGGGTGGCGCTGCAGGTCTGCGCCCAGCCGCT
GGAAATGCTGGCGCGGCGCGATGTGCGCAAGCTGATGCAGGCCTGCGGCCTGCCCGAGGCCAAGGTGCGCGCCGCGCTGG
GCGTCATCGCCCGGCTGGAGCCCAAGCCGGGGCGGCGCTTCACCGATGTCGAGCGCCACATCATCGTGCCCGACGTGATC
GTCAAGAAAACAGCTCGCAAGGGGGGCGATGGCCTGGCGCAGTTCAGCGTGCAGCTGAACCCGGACGTGATGCCGCGCCT
GCGCGTGCACGACGTCTACGCCAACGCCCTGCGTAGCCACAAGGGCAGTGACGGGCACGCCGGACTGCAGCAGCGGCTGC
AGGAGGCGCGCTGGTTCATCAAGAACGTGCAGCAGCGCTTCGACACCATCCTGCGTGTGTCCAAGGCCATCGTCGACCGG
CAGAAGAACTTTTTCGTGCACGGCGAGCTGGCCATGCGCCCGCTGGTGCTGCGCGACATTGCCGACGAGCTGGGCCTGCA
CGAATCGACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCACGCCGCACGGCACCTACGAGCTGAAGTTCTTCTTCG
GATCGGGCCTGGGCACGGAGAACGGGGGCAATGCCTCCAGCACCGCGGTGCGCGCGCTGATCAAGCAGTTCGTTGCGGCC
GAGAGCCCGAAGAAACCGCTGTCCGACAGCCAGATCGCGGACATGCTCAAGGAGCAGGGCATCGAGTGCGCGCGCCGCAC
CGTGGCCAAGTACCGCGAGGCCTTGAAGATCGCCCCGGCCAACCTGCGCAAGGCGCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(369-524)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(148-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.72

100

0.534