Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N1249_RS19840 Genome accession   NZ_CP104014
Coordinates   4203936..4205369 (+) Length   477 a.a.
NCBI ID   WP_259832406.1    Uniprot ID   -
Organism   Enterobacter sp. CP102     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4198936..4210369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N1249_RS19810 (N1249_19810) - 4199503..4200480 (+) 978 WP_014071947.1 calcium/sodium antiporter -
  N1249_RS19815 (N1249_19815) kdsD 4200494..4201480 (+) 987 WP_014071948.1 arabinose-5-phosphate isomerase KdsD -
  N1249_RS19820 (N1249_19820) kdsC 4201495..4202061 (+) 567 WP_014071949.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  N1249_RS19825 (N1249_19825) lptC 4202058..4202633 (+) 576 WP_259832375.1 LPS export ABC transporter periplasmic protein LptC -
  N1249_RS19830 (N1249_19830) lptA 4202602..4203156 (+) 555 WP_259832384.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  N1249_RS19835 (N1249_19835) lptB 4203163..4203888 (+) 726 WP_014071952.1 LPS export ABC transporter ATP-binding protein -
  N1249_RS19840 (N1249_19840) rpoN/rpoN1 4203936..4205369 (+) 1434 WP_259832406.1 RNA polymerase factor sigma-54 Machinery gene
  N1249_RS19845 (N1249_19845) hpf 4205392..4205679 (+) 288 WP_166718038.1 ribosome hibernation promoting factor -
  N1249_RS19850 (N1249_19850) ptsN 4205762..4206253 (+) 492 WP_014071955.1 PTS IIA-like nitrogen regulatory protein PtsN -
  N1249_RS19855 (N1249_19855) rapZ 4206299..4207153 (+) 855 WP_182062681.1 RNase adapter RapZ -
  N1249_RS19860 (N1249_19860) npr 4207150..4207422 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  N1249_RS19865 (N1249_19865) mtgA 4207462..4208187 (-) 726 WP_259832413.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  N1249_RS19870 (N1249_19870) elbB 4208184..4208837 (-) 654 WP_259838225.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54163.96 Da        Isoelectric Point: 4.3332

>NTDB_id=727031 N1249_RS19840 WP_259832406.1 4203936..4205369(+) (rpoN/rpoN1) [Enterobacter sp. CP102]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQTQDTETLDTVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVSLDD
ILESIGDDEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFAKETPWIEEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFDQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=727031 N1249_RS19840 WP_259832406.1 4203936..4205369(+) (rpoN/rpoN1) [Enterobacter sp. CP102]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCACAGCTACAACAAGCTATCCGCCTGTTGCA
GCTGTCCACGTTAGAACTACAGCAGGAACTCCAGCAAGCACTGGACAGTAACCCGTTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACACTCAGCAAACGCAAGACACCGAAACGCTCGATACCGTTGATGCTCTCGAACAAAAAGAGATGCCGGAC
GAGCTGCCTCTGGATGCCAGCTGGGATGAAATCTACACCGCAGGAACCCCATCCGGGACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGTGAAACCACCCAGTCGCTTCAGGATTACCTGATGTGGCAGGTGGAGTTAACCCCCTTCT
CTGACACCGATCGTGCAATTGCGACGTCCATTGTCGATGCCGTTGATGACACCGGATATTTGACCGTTTCTCTGGACGAT
ATTCTTGAGAGCATCGGCGATGATGAGATCGAACTCGAGGAAATTGAAGCGGTTCTGAAGCGTATTCAGCGCTTCGACCC
GGTTGGCGTGGCCGCGAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCGCCAAAGAGACCCCGTGGATTG
AAGAAGCCCGGTTAATCATCAGCGATCATCTGGATCTGCTGGCGAACCATGACTTCCGCACCCTGATGCGCGTCACCCGC
CTTAAAGAAGAGGTGTTGAAAGAAGCCGTCAACCTGATCCAGTCGCTCGACCCTCGCCCTGGCCAATCCATCCAGACCAG
CGAACCCGAATATGTAATCCCGGATGTGCTGGTCAGAAAGCATAACGGCCGCTGGGTTGTCGAACTGAATTCCGACAGCA
TTCCTCGCCTGCAAATCAATCAGCAGTACGCCTCCATGTGCACCAGCGCACGCAACGACTCCGACAACCAGTACATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAGAGTCTGGAGAGCAGAAATGATACGCTGCTCCGTGTAAGCCGCTGTAT
TGTCGAGCAGCAGCAGGCGTTCTTTGATCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCAGATATCGCCCAGGCCG
TCGAAATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAATACCGAAGGCGGCGGCGAAGCCTCGTCGACGGCCATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAATCCCGCGAAGCCACTGAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATCATGGTGG
CACGCCGTACTGTTGCAAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48