Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   N0B18_RS13595 Genome accession   NZ_CP103995
Coordinates   2512885..2513295 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain SRCM117109     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2507885..2518295
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N0B18_RS13565 (N0B18_13565) - 2507971..2508111 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  N0B18_RS13570 (N0B18_13570) yqeF 2508417..2509148 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  N0B18_RS13575 (N0B18_13575) cwlH 2509400..2510152 (-) 753 WP_069964218.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  N0B18_RS13580 (N0B18_13580) yqeD 2510339..2510965 (+) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  N0B18_RS13585 (N0B18_13585) gnd 2510985..2511878 (-) 894 WP_259606772.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  N0B18_RS13590 (N0B18_13590) yqeB 2512130..2512852 (+) 723 WP_014480321.1 hypothetical protein -
  N0B18_RS13595 (N0B18_13595) nucA/comI 2512885..2513295 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  N0B18_RS13600 (N0B18_13600) sigK 2513491..2514219 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  N0B18_RS13605 (N0B18_13605) - 2514219..2514317 (+) 99 WP_031600702.1 hypothetical protein -
  N0B18_RS13610 (N0B18_13610) - 2514314..2514526 (-) 213 Protein_2636 recombinase family protein -
  N0B18_RS13615 (N0B18_13615) fumC 2514745..2516133 (-) 1389 WP_014480325.1 class II fumarate hydratase -
  N0B18_RS13620 (N0B18_13620) - 2516300..2517190 (+) 891 WP_014480326.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=726852 N0B18_RS13595 WP_009967785.1 2512885..2513295(-) (nucA/comI) [Bacillus subtilis strain SRCM117109]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=726852 N0B18_RS13595 WP_009967785.1 2512885..2513295(-) (nucA/comI) [Bacillus subtilis strain SRCM117109]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529