Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   N0B18_RS00580 Genome accession   NZ_CP103995
Coordinates   105037..106407 (+) Length   456 a.a.
NCBI ID   WP_069963943.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM117109     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 100037..111407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N0B18_RS00560 (N0B18_00560) ctsR 100390..100854 (+) 465 WP_003225724.1 transcriptional regulator CtsR -
  N0B18_RS00565 (N0B18_00565) mcsA 100868..101425 (+) 558 WP_015252993.1 protein-arginine kinase activator protein McsA -
  N0B18_RS00570 (N0B18_00570) mcsB 101425..102516 (+) 1092 WP_003235007.1 protein arginine kinase -
  N0B18_RS00575 (N0B18_00575) clpC 102513..104945 (+) 2433 WP_014475592.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  N0B18_RS00580 (N0B18_00580) radA 105037..106407 (+) 1371 WP_069963943.1 DNA repair protein RadA Machinery gene
  N0B18_RS00585 (N0B18_00585) disA 106411..107493 (+) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  N0B18_RS00590 (N0B18_00590) yacL 107609..108709 (+) 1101 WP_003235014.1 PIN/TRAM domain-containing protein -
  N0B18_RS00595 (N0B18_00595) ispD 108724..109422 (+) 699 WP_014478616.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  N0B18_RS00600 (N0B18_00600) ispF 109415..109891 (+) 477 WP_003225745.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49322.86 Da        Isoelectric Point: 8.2399

>NTDB_id=726804 N0B18_RS00580 WP_069963943.1 105037..106407(+) (radA) [Bacillus subtilis strain SRCM117109]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYISSA
IQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYFEG
ERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSFGN
PRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIVISIASSFRDTPPNPADCFIGEVGLTGE
VRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=726804 N0B18_RS00580 WP_069963943.1 105037..106407(+) (radA) [Bacillus subtilis strain SRCM117109]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATTGGCGGTGATCCTGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCATCAAACAGTGTCCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAACGAAGC
TGCGAGCAGACCGTCTGGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCGTCTGCT
ATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCGGCTCCAGG
CAGTGTGTCACAGGTCAGAGAATGTACTGCTGAGCTGATGAAAATCGCAAAAACAAAAGGTATTCCGATTTTTATCGTAG
GGCACGTGACGAAAGAAGGGTCTATTGCAGGTCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTTGAAGGA
GAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATTTTCGAAAT
GCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCGGAAATTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAGGCTCGA
GTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCGCTCATCTCGCCAACAAGCTTTGGCAAC
CCAAGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCTCTGTTAATGGCTGTGTTAGAAAAAAGAGTTGGACTGCT
GCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCGATTGTGA
TTAGTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGTTTTATTGGAGAAGTGGGATTAACCGGAGAA
GTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGCTTTAAACGCATGATCATACCCGCGGC
AAATCTGGACGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTACTTCAT
TAGGAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-220)

Subunit ChlI of Mg-chelatase (ChlI)

(363-428)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.345

100

0.998
  radA Streptococcus pneumoniae Rx1

63.274

99.123

0.627
  radA Streptococcus pneumoniae D39

63.274

99.123

0.627
  radA Streptococcus pneumoniae R6

63.274

99.123

0.627
  radA Streptococcus pneumoniae TIGR4

63.274

99.123

0.627
  radA Streptococcus mitis NCTC 12261

63.274

99.123

0.627
  radA Streptococcus mitis SK321

63.053

99.123

0.625