Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   FGKAn22_RS02100 Genome accession   NZ_AP019536
Coordinates   455175..456608 (-) Length   477 a.a.
NCBI ID   WP_212786335.1    Uniprot ID   A0AAN1VZ10
Organism   Ferrigenium kumadai strain An22     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 450175..461608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGKAn22_RS02065 (FGKAn22_04060) - 450966..451568 (-) 603 WP_281411889.1 flavin prenyltransferase UbiX -
  FGKAn22_RS02070 (FGKAn22_04070) - 451565..452047 (-) 483 WP_246487475.1 Gx transporter family protein -
  FGKAn22_RS02075 (FGKAn22_04080) - 452160..452558 (-) 399 WP_246487439.1 NusG domain II-containing protein -
  FGKAn22_RS02080 (FGKAn22_04090) rapZ 452545..453381 (-) 837 WP_212786331.1 RNase adapter RapZ -
  FGKAn22_RS02085 (FGKAn22_04100) hprK 453383..454321 (-) 939 WP_212786332.1 HPr(Ser) kinase/phosphatase -
  FGKAn22_RS02090 (FGKAn22_04110) - 454323..454781 (-) 459 WP_212786333.1 PTS sugar transporter subunit IIA -
  FGKAn22_RS02095 (FGKAn22_04120) raiA 454830..455162 (-) 333 WP_212786334.1 ribosome-associated translation inhibitor RaiA -
  FGKAn22_RS02100 (FGKAn22_04130) rpoN/rpoN1 455175..456608 (-) 1434 WP_212786335.1 RNA polymerase factor sigma-54 Machinery gene
  FGKAn22_RS02105 (FGKAn22_04140) lptB 456641..457363 (-) 723 WP_212786336.1 LPS export ABC transporter ATP-binding protein -
  FGKAn22_RS02110 (FGKAn22_04150) lptA 457366..457956 (-) 591 WP_212786337.1 lipopolysaccharide transport periplasmic protein LptA -
  FGKAn22_RS02115 (FGKAn22_04160) lptC 457937..458518 (-) 582 WP_212786338.1 LPS export ABC transporter periplasmic protein LptC -
  FGKAn22_RS02120 (FGKAn22_04170) - 458515..459033 (-) 519 WP_212786339.1 HAD family hydrolase -
  FGKAn22_RS02125 (FGKAn22_04180) - 459043..460029 (-) 987 WP_212786340.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53404.31 Da        Isoelectric Point: 5.0286

>NTDB_id=72668 FGKAn22_RS02100 WP_212786335.1 455175..456608(-) (rpoN/rpoN1) [Ferrigenium kumadai strain An22]
MKASLQLRLSQQLTLTPQLQQAIRLLQLSTQDIHQEVARMLDENPLLEAAEEPASAIYSPGTSSMESRGSASNDEDGTSS
RDERDVDGLASEPAEWSSRTARSADDEDDSYPEQAAEQASLREHLHNQLTTSSLDERDRKVVSLLIDALDENGYLVQDLA
ELAELLPAELAISLDDLETALVQLQHLDQPGLGARNLGECLALQLKALPEDTPQRDLSISLVSRHLDLLAAHDFARIKKL
LRCSDDQLRSAQHLIIGLNPKPGAEFDQSVADYVVPDVIVEKHKNQWRARLNTEAMPKLRINQIYANILQQRDDKNASQL
ATQLQEAKWLIKNLQQRFETILRVAQAIVDRQTNFLEHGEIGMRPLVLREIAEALDLHESTVSRSTTQKFMLTPRGIYEF
KHFFGSGLATESGGTCSSTAIRELIKQMVSAEDRRKPLTDSRMSEILAQQGIVVARRTIAKYREALHILPVNLRKSL

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=72668 FGKAn22_RS02100 WP_212786335.1 455175..456608(-) (rpoN/rpoN1) [Ferrigenium kumadai strain An22]
ATGAAGGCATCTCTCCAACTCAGGCTGTCCCAGCAACTGACGCTTACCCCGCAGTTGCAACAGGCTATCCGTTTGCTGCA
GCTCTCCACGCAGGACATCCACCAGGAAGTCGCCCGCATGCTGGACGAAAACCCCTTGCTGGAAGCGGCAGAGGAACCCG
CATCGGCCATCTATTCCCCCGGCACCTCATCTATGGAATCGCGCGGCAGCGCAAGCAACGACGAGGATGGCACATCGTCT
CGCGACGAGCGCGATGTGGACGGTCTGGCCAGCGAACCCGCCGAGTGGAGCAGCAGGACTGCGCGCAGCGCCGACGACGA
AGACGACAGCTACCCGGAACAGGCCGCTGAACAGGCCAGCCTGCGCGAACATCTGCACAATCAACTGACCACCAGTTCAC
TGGACGAACGCGACCGCAAGGTGGTCAGCCTGCTGATCGATGCGCTGGACGAGAACGGTTACCTGGTACAGGACCTGGCC
GAACTTGCCGAACTGTTGCCCGCCGAACTGGCGATCTCTCTGGATGACCTGGAGACCGCACTGGTGCAATTGCAGCACCT
CGACCAGCCCGGGCTGGGAGCGCGCAACCTGGGCGAATGCCTCGCGCTGCAGCTCAAGGCGCTGCCGGAAGATACGCCGC
AACGCGACCTGTCGATCAGCCTGGTGAGCCGACATCTCGACCTGCTCGCCGCCCACGATTTCGCCAGGATCAAGAAGCTG
CTGCGCTGCTCAGATGACCAGTTGCGCAGCGCACAGCACCTGATCATCGGGCTCAACCCGAAGCCGGGCGCGGAATTCGA
CCAGAGTGTCGCGGACTACGTGGTCCCGGACGTGATCGTGGAGAAGCACAAGAACCAGTGGCGCGCCCGCCTCAACACGG
AAGCGATGCCCAAACTGCGCATCAACCAGATCTACGCCAACATCCTGCAGCAGCGCGACGACAAGAACGCCTCGCAGCTC
GCCACCCAGCTGCAGGAAGCGAAGTGGCTGATCAAGAACCTGCAACAGCGTTTCGAGACCATCTTGCGCGTGGCTCAGGC
CATCGTGGATCGGCAGACCAACTTTCTCGAGCACGGCGAGATCGGCATGCGCCCGCTGGTGCTGCGCGAGATCGCCGAGG
CGCTCGACCTGCACGAGTCGACCGTCTCGCGCAGCACCACGCAGAAATTCATGCTGACGCCGCGCGGCATCTACGAATTC
AAGCATTTCTTTGGCAGCGGACTGGCCACGGAAAGCGGCGGCACCTGTTCTTCCACCGCAATACGCGAACTGATCAAACA
AATGGTCAGCGCGGAAGACCGGCGCAAACCGCTTACCGACAGCCGCATGTCGGAAATTTTGGCACAGCAGGGCATCGTAG
TCGCCCGGCGTACCATAGCAAAGTACCGGGAAGCGCTGCACATACTCCCGGTGAACCTCCGTAAATCACTCTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(113-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.69

100

0.545

Multiple sequence alignment