Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PWR57_RS21545 Genome accession   NZ_CP119441
Coordinates   4270791..4272224 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli O121 strain 201805972     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4265791..4277224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWR57_RS21515 (PWR57_21485) yrbG 4266349..4267326 (+) 978 WP_000922901.1 calcium/sodium antiporter -
  PWR57_RS21520 (PWR57_21490) kdsD 4267340..4268326 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  PWR57_RS21525 (PWR57_21495) kdsC 4268347..4268913 (+) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PWR57_RS21530 (PWR57_21500) lptC 4268910..4269485 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  PWR57_RS21535 (PWR57_21505) lptA 4269454..4270011 (+) 558 WP_146367935.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  PWR57_RS21540 (PWR57_21510) lptB 4270018..4270743 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  PWR57_RS21545 (PWR57_21515) rpoN/rpoN1 4270791..4272224 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  PWR57_RS21550 (PWR57_21520) hpf 4272247..4272534 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  PWR57_RS21555 (PWR57_21525) ptsN 4272652..4273143 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PWR57_RS21560 (PWR57_21530) rapZ 4273189..4274043 (+) 855 WP_000243741.1 RNase adapter RapZ -
  PWR57_RS21565 (PWR57_21535) npr 4274040..4274312 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  PWR57_RS21570 (PWR57_21540) yrbL 4274526..4275158 (+) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  PWR57_RS21575 (PWR57_21545) mtgA 4275155..4275883 (-) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  PWR57_RS21580 (PWR57_21550) elbB 4275880..4276533 (-) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=726491 PWR57_RS21545 WP_000809051.1 4270791..4272224(+) (rpoN/rpoN1) [Escherichia coli O121 strain 201805972]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=726491 PWR57_RS21545 WP_000809051.1 4270791..4272224(+) (rpoN/rpoN1) [Escherichia coli O121 strain 201805972]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGATTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACAGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCTCGCAATGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465