Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PX810_RS01260 Genome accession   NZ_CP119402
Coordinates   273873..275369 (+) Length   498 a.a.
NCBI ID   WP_001040656.1    Uniprot ID   -
Organism   Escherichia coli strain MRE162 substr. FRA     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 268873..280369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PX810_RS01230 (PX810_000246) tusA 269120..269365 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  PX810_RS01235 (PX810_000247) zntA 269467..271665 (-) 2199 WP_000106551.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  PX810_RS01240 (PX810_000248) yhhN 271739..272365 (-) 627 WP_097450986.1 lysoplasmalogenase -
  PX810_RS01245 (PX810_000249) yhhM 272506..272865 (+) 360 WP_000042895.1 DUF2500 domain-containing protein -
  PX810_RS01250 (PX810_000250) yhhL 272868..273137 (-) 270 WP_001311191.1 DUF1145 family protein -
  PX810_RS01255 (PX810_000251) rsmD 273127..273723 (-) 597 WP_063091543.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  PX810_RS01260 (PX810_000252) pilA 273873..275369 (+) 1497 WP_001040656.1 signal recognition particle-docking protein FtsY Machinery gene
  PX810_RS01265 (PX810_000253) ftsE 275372..276040 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  PX810_RS01270 (PX810_000254) ftsX 276033..277091 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  PX810_RS01275 (PX810_000255) rpoH 277336..278190 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  PX810_RS01280 (PX810_000256) livJ 278461..279564 (+) 1104 WP_001022007.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  PX810_RS01285 (PX810_000257) panM 279764..280147 (-) 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54699.51 Da        Isoelectric Point: 4.1468

>NTDB_id=726176 PX810_RS01260 WP_001040656.1 273873..275369(+) (pilA) [Escherichia coli strain MRE162 substr. FRA]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=726176 PX810_RS01260 WP_001040656.1 273873..275369(+) (pilA) [Escherichia coli strain MRE162 substr. FRA]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAACCTGTGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATAATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACTGGTGCGGATTCCGCTTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCATCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378