Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NX081_RS09110 Genome accession   NZ_CP103856
Coordinates   1860594..1861028 (+) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain SRCM116265     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1855594..1866028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX081_RS09105 (NX081_09105) - 1859094..1860305 (-) 1212 WP_060561839.1 cytochrome P450 -
  NX081_RS09110 (NX081_09110) nucA/comI 1860594..1861028 (+) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NX081_RS09115 (NX081_09115) - 1861088..1861843 (+) 756 WP_060561664.1 YoaK family protein -
  NX081_RS09120 (NX081_09120) - 1861877..1862239 (-) 363 WP_007410383.1 hypothetical protein -
  NX081_RS09125 (NX081_09125) - 1862433..1863761 (-) 1329 WP_060561663.1 S8 family peptidase -
  NX081_RS09130 (NX081_09130) - 1863940..1864173 (+) 234 WP_060561662.1 hypothetical protein -
  NX081_RS09135 (NX081_09135) - 1864429..1865136 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  NX081_RS09140 (NX081_09140) - 1865196..1865648 (+) 453 WP_014305039.1 OsmC family protein -
  NX081_RS09145 (NX081_09145) - 1865662..1866015 (-) 354 WP_029973435.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=726085 NX081_RS09110 WP_007611576.1 1860594..1861028(+) (nucA/comI) [Bacillus velezensis strain SRCM116265]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=726085 NX081_RS09110 WP_007611576.1 1860594..1861028(+) (nucA/comI) [Bacillus velezensis strain SRCM116265]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGAGC
GGGCGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCTGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486