Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NY025_RS23620 Genome accession   NZ_CP103852
Coordinates   3384750..3386249 (+) Length   499 a.a.
NCBI ID   WP_193026583.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain LMG 9673     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3379750..3391249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NY025_RS23595 (NY025_23595) - 3380949..3381932 (+) 984 WP_193026578.1 SIS domain-containing protein -
  NY025_RS23600 (NY025_23600) - 3381964..3382551 (+) 588 WP_193026579.1 HAD family hydrolase -
  NY025_RS23605 (NY025_23605) lptC 3382553..3383185 (+) 633 WP_193026580.1 LPS export ABC transporter periplasmic protein LptC -
  NY025_RS23610 (NY025_23610) lptA 3383255..3383830 (+) 576 WP_193026581.1 lipopolysaccharide transport periplasmic protein LptA -
  NY025_RS23615 (NY025_23615) lptB 3383864..3384652 (+) 789 WP_193026582.1 LPS export ABC transporter ATP-binding protein -
  NY025_RS23620 (NY025_23620) rpoN/rpoN1 3384750..3386249 (+) 1500 WP_193026583.1 RNA polymerase factor sigma-54 Machinery gene
  NY025_RS23625 (NY025_23625) hpf 3386301..3386654 (+) 354 WP_193026584.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  NY025_RS23630 (NY025_23630) ptsN 3387003..3387458 (+) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NY025_RS23635 (NY025_23635) hprK 3387529..3388503 (+) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  NY025_RS23640 (NY025_23640) - 3388692..3389027 (+) 336 WP_193028556.1 PsiF family protein -
  NY025_RS23645 (NY025_23645) rapZ 3389121..3390011 (+) 891 WP_193026585.1 RNase adapter RapZ -
  NY025_RS23650 (NY025_23650) - 3390089..3390739 (+) 651 WP_193026586.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55184.55 Da        Isoelectric Point: 4.8441

>NTDB_id=726025 NY025_RS23620 WP_193026583.1 3384750..3386249(+) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain LMG 9673]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVSALQSSSATPMPTE
SPPPTANGADHPDTAEASYENGSDFDSDYGTDRSDWSLDDFARKPQSDEDDRAPMQLRDAEPTQREFLMEQLAPLKLSPR
DKGLVIFLIESLDDEGYLTGSLDEILADLPPELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
AIVNQHLELLAARDYTRLKKALSVDDGALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=726025 NY025_RS23620 WP_193026583.1 3384750..3386249(+) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain LMG 9673]
ATGAAACAGTCGCTCCAGCTCCGCCTTTCCCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAACTGCAACAGGAAGTCGAGCAGGCGCTGACTGAGAACCCGCTGCTTGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGACGGCTCGGTCAGCGCCCTGCAGAGCAGCAGTGCCACCCCGATGCCGACCGAA
TCGCCGCCGCCCACCGCCAACGGCGCGGACCATCCCGACACGGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTACGGCACCGATCGGTCCGACTGGAGCCTCGACGACTTCGCGCGCAAACCCCAGTCCGACGAAGACGACCGCGCGC
CGATGCAGCTGCGCGATGCCGAACCCACGCAGCGCGAATTCCTGATGGAACAGCTCGCGCCGCTCAAGCTGTCCCCGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGATCTGCCGCCCGAACTCGAGGTCGAGCTCGATGAATTGCAAGCCGCGCTGCGGATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGTGCGCGCTCGGCGGCCGAATGCCTGACGCTGCAGCTGCAGCGCCTGGACTCCCCAGCCAAGCCGCTGGCGCTG
GCCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGGCTGAAGAAAGCGCTGTCCGTCGATGATGG
CGCGCTGAAGGCCGCGCATGAGCTGATCCGCTCGCTGGCGCCCTTCCCGGGCCACGCCTTCGGGCGGGCCGAGGCCGACT
TCGTCGTGCCCGACGTGGTGGTGCGCAAGACCGCCGCGGGCTGGATGGCACAGCTGAACCCCGACGTGATGCCACGCCTG
CGCATCAACGACATGTATGCGCAGATCCTGCGCAGCAGCCGTGGCGAATCGGGCGCCGCCAACCTGCAGCAGAAGCTGCA
AGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCCATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAATACATGGCAACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAGATCCCAGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

98.196

100

0.982