Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   NYR71_RS05675 Genome accession   NZ_CP103817
Coordinates   1288077..1289375 (-) Length   432 a.a.
NCBI ID   WP_279438017.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 4005     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1290314..1303836 1288077..1289375 flank 939


Gene organization within MGE regions


Location: 1288077..1303836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR71_RS05675 (NYR71_05675) comE 1288077..1289375 (-) 1299 WP_279438017.1 type IV pilus secretin PilQ Machinery gene
  NYR71_RS05680 (NYR71_05680) - 1289387..1289791 (-) 405 WP_279439255.1 hypothetical protein -
  NYR71_RS05685 (NYR71_05685) - 1289784..1290311 (-) 528 WP_279439256.1 chromosome segregation protein -
  NYR71_RS05690 (NYR71_05690) - 1290314..1290835 (-) 522 WP_279439257.1 hypothetical protein -
  NYR71_RS05695 (NYR71_05695) - 1290817..1291503 (-) 687 WP_039197447.1 hypothetical protein -
  NYR71_RS05700 (NYR71_05700) - 1291642..1294227 (+) 2586 WP_279439258.1 penicillin-binding protein 1A -
  NYR71_RS05705 (NYR71_05705) aroK 1294491..1295012 (+) 522 WP_005623982.1 shikimate kinase AroK -
  NYR71_RS05710 (NYR71_05710) aroB 1295029..1296117 (+) 1089 WP_039197450.1 3-dehydroquinate synthase -
  NYR71_RS05715 (NYR71_05715) - 1296121..1296957 (+) 837 WP_039197452.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  NYR71_RS05720 (NYR71_05720) - 1297027..1298385 (+) 1359 WP_279436626.1 sodium-dependent transporter -
  NYR71_RS05725 (NYR71_05725) pgi 1298513..1300153 (-) 1641 WP_279439259.1 glucose-6-phosphate isomerase -
  NYR71_RS05730 (NYR71_05730) - 1300192..1300443 (-) 252 WP_039197458.1 accessory factor UbiK family protein -
  NYR71_RS05755 (NYR71_05755) aroE 1301161..1301985 (-) 825 WP_279439260.1 shikimate dehydrogenase -
  NYR71_RS05760 (NYR71_05760) - 1301991..1302545 (-) 555 WP_279439261.1 Sua5/YciO/YrdC/YwlC family protein -
  NYR71_RS05765 (NYR71_05765) - 1302538..1303071 (-) 534 WP_279439262.1 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
  NYR71_RS05770 (NYR71_05770) yqfB 1303071..1303376 (-) 306 WP_279439263.1 N(4)-acetylcytidine aminohydrolase -
  NYR71_RS05775 (NYR71_05775) recX 1303369..1303824 (-) 456 WP_237610172.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 47694.82 Da        Isoelectric Point: 9.4293

>NTDB_id=725445 NYR71_RS05675 WP_279438017.1 1288077..1289375(-) (comE) [Actinobacillus equuli subsp. equuli strain 4005]
MRRVILLLLLILTPVMANTFSISLKNAPTAEVLSYLAEEYGKNIVLSGNIETNTTLRIENSDFDSVLKSITRANKLSSAY
ENQIYFIGHKKDEKAATPIAVNPDVLKPKLITKTIKLDYAKAAEVIESLTKGSGNFLSENGYLHFDDRSNSLIIKDSPES
MKNIVKLIRNLDKPTEQIAIEARIVTISSENLQELGVRWGMFSPTNGHHKVAGSLEANGLPNTNHLNVNFPVNNAASIAL
QVAKINGRVLDLELTALEQENDVEIIASPRLLTTNKKPASIKQGTEIPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDN
QILLDLVVTQNSPNSTSSTVHGLVTIDKQELNTQVFAKHGETIVLGGIFQHLTAKGEDRMPILGSIPVIKKLFSHSSDRI
SKRELVIFVTPYIVKSGKQQISSHSSQKLPPK

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=725445 NYR71_RS05675 WP_279438017.1 1288077..1289375(-) (comE) [Actinobacillus equuli subsp. equuli strain 4005]
ATGCGAAGAGTGATTTTGTTGCTCCTTTTGATATTAACGCCGGTTATGGCAAATACTTTTTCTATCTCGCTGAAAAATGC
GCCGACAGCGGAAGTTTTAAGTTATTTAGCCGAAGAATACGGCAAAAATATTGTGTTAAGCGGCAATATTGAAACAAATA
CTACATTAAGAATTGAAAATAGCGATTTTGATAGCGTGTTAAAAAGTATTACTCGAGCAAATAAGCTCAGCAGTGCGTAT
GAAAACCAGATCTATTTTATTGGACATAAAAAAGATGAGAAAGCGGCTACGCCAATAGCTGTAAATCCGGATGTATTGAA
ACCTAAGTTGATTACTAAGACAATAAAATTAGATTATGCGAAGGCAGCGGAAGTGATTGAATCTTTAACCAAAGGTAGTG
GTAATTTTTTATCGGAAAACGGCTATCTACATTTTGATGATCGTAGTAATAGTTTGATAATTAAAGATAGCCCAGAATCG
ATGAAAAATATCGTGAAATTAATTAGAAATCTGGATAAACCGACTGAGCAGATTGCGATTGAAGCAAGGATAGTCACAAT
AAGTAGTGAAAATTTGCAAGAGCTTGGGGTACGCTGGGGAATGTTTTCTCCGACAAACGGACATCATAAAGTCGCTGGTT
CGCTTGAAGCGAATGGACTACCGAATACTAACCATTTAAACGTAAATTTTCCGGTAAATAATGCCGCTTCTATTGCGCTA
CAAGTGGCAAAAATTAATGGACGAGTGCTTGATTTGGAATTAACCGCTTTAGAGCAAGAAAATGATGTGGAAATTATTGC
CAGCCCTCGTTTACTGACCACTAATAAGAAACCGGCGAGTATTAAGCAAGGGACTGAAATTCCGTATGTGCTTTATAACC
GTAAAGATGAAGTGAAAAATATCGAATTTAAAGAAGCCGTTTTAGGGCTACAGGTCACGCCGCATATTTCAAACGATAAT
CAAATTTTGCTTGATTTGGTGGTGACACAAAATTCGCCGAATTCAACCAGTTCGACAGTTCATGGTTTAGTAACGATTGA
TAAACAGGAATTAAATACGCAAGTATTCGCTAAGCATGGTGAAACTATTGTGCTAGGCGGTATTTTTCAGCATTTAACCG
CAAAAGGTGAGGACAGAATGCCGATTTTAGGTTCAATTCCGGTCATTAAAAAGCTATTTAGCCATTCCAGTGATAGGATC
AGTAAGCGCGAATTAGTTATTTTCGTTACACCTTATATTGTTAAAAGTGGAAAACAGCAAATTTCCTCGCATTCTTCACA
GAAATTACCGCCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(256-415)

Bacterial type II/III secretion system short domain (Secretin_N)

(112-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

66.427

96.528

0.641
  comE Haemophilus influenzae 86-028NP

54.415

96.991

0.528
  comE Haemophilus influenzae Rd KW20

55.583

93.287

0.519
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.614

96.065

0.419
  pilQ Vibrio cholerae strain A1552

43.614

96.065

0.419
  pilQ Vibrio campbellii strain DS40M4

41.469

97.685

0.405