Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   PY824_RS04275 Genome accession   NZ_CP119172
Coordinates   777222..779429 (+) Length   735 a.a.
NCBI ID   WP_014294320.1    Uniprot ID   A0A2G3NWI2
Organism   Streptococcus macedonicus strain SGM CIP105683     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 772222..784429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY824_RS04250 (PY824_04250) miaA 772561..773448 (+) 888 WP_115266056.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  PY824_RS04255 (PY824_04255) hflX 773587..774825 (+) 1239 WP_014294316.1 GTPase HflX -
  PY824_RS04260 (PY824_04260) - 774818..775456 (+) 639 WP_014294317.1 cystathionine beta-lyase -
  PY824_RS04265 (PY824_04265) rnz 775496..776425 (+) 930 WP_014294318.1 ribonuclease Z -
  PY824_RS04270 (PY824_04270) - 776422..777180 (+) 759 WP_014294319.1 SDR family oxidoreductase -
  PY824_RS04275 (PY824_04275) recJ 777222..779429 (+) 2208 WP_014294320.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  PY824_RS04280 (PY824_04280) - 779991..780116 (+) 126 WP_014294322.1 hypothetical protein -
  PY824_RS04285 (PY824_04285) - 780207..780824 (-) 618 WP_250575525.1 hypothetical protein -
  PY824_RS04290 (PY824_04290) - 781363..782067 (-) 705 WP_258927441.1 CPBP family intramembrane glutamic endopeptidase -
  PY824_RS04295 (PY824_04295) bsh 782343..783327 (+) 985 Protein_777 choloylglycine hydrolase -
  PY824_RS04300 (PY824_04300) - 783344..783871 (+) 528 WP_014294326.1 hypothetical protein -

Sequence

Protein


Download         Length: 735 a.a.        Molecular weight: 82504.15 Da        Isoelectric Point: 4.3607

>NTDB_id=725419 PY824_RS04275 WP_014294320.1 777222..779429(+) (recJ) [Streptococcus macedonicus strain SGM CIP105683]
MITSKYNWKNIEKEPDNGFFELTKKEKLTELASQILYSRGVDTAEKMNQFLSTDLSQLHDPYLLHDMDKAVVRIRQAIEN
YEQILVYGDYDADGMTSASIMKEALEMLGAEAQVYLPNRFTDGYGPNESVYKYFIEQQNVSLIITVDNGVAGHEAIAYAQ
SQGVDVIVTDHHSLPVELPEAFAIVHPEHPKADYPFKYLAGCGVAFKVACALLESIPTEMLDLVAIGTIADMVSLTDENR
IMVKVGLEILKQTERIGLIELMKVSDVDMADVNEETVGFKIAPQLNALGRLDDPNPAVELLTGFDDEEAHNIAKMINAKN
EERKEIVQKIFDEAMSMVDLEKPVQVLAKEGWHPGVLGIVAGRISEQIAQPVIVLNIEDGLAKGSARSIETINIFHALDE
HRDLFEAFGGHAGAAGMTLVTENLDKLLQVLCDYIAENDIDLSQKKELVIDEVLELSEIDLDTVQSLEKLAPFGMDNPKP
VFEIKDFVVKQARTMGQNGAHLKLKIAQGTTAIDLVAFNQGHLAQEFQQAQNLCLATTLSINKWNGQMTVQLMLEDAHVE
GVQLIDIRSKTASLPERVPVLAEDSTANEVVVLDIPDKAEELKSLFVGRHFEAVYFKNHIKRAYYLTGYGTRDQFAKLYK
TIYQFPEFDVRYKLKDLSQYLNIPDILLIKMIQIFDELDFVTITDGVMVVNKDAQKRSISESQIYQDLKQQVEFQELMAL
GTPQEIYDWLTDEDD

Nucleotide


Download         Length: 2208 bp        

>NTDB_id=725419 PY824_RS04275 WP_014294320.1 777222..779429(+) (recJ) [Streptococcus macedonicus strain SGM CIP105683]
ATGATAACAAGTAAATACAATTGGAAAAATATTGAAAAAGAGCCAGATAATGGCTTTTTTGAACTGACAAAAAAAGAAAA
GCTAACAGAATTAGCTAGTCAGATTCTTTATAGCCGAGGTGTTGATACGGCAGAAAAAATGAATCAATTTTTATCAACGG
ATTTGTCACAATTGCATGATCCTTACCTTTTACATGACATGGATAAAGCGGTTGTGCGTATTCGCCAAGCCATTGAGAAC
TACGAGCAAATTTTGGTTTATGGTGATTATGATGCTGACGGAATGACTAGTGCCAGTATTATGAAAGAAGCCCTTGAAAT
GTTAGGCGCTGAAGCTCAAGTTTATCTGCCAAATCGTTTCACAGACGGATACGGTCCCAATGAATCGGTCTATAAATATT
TCATTGAGCAGCAAAACGTTTCTTTGATTATCACGGTGGACAATGGCGTAGCAGGGCACGAAGCGATTGCCTACGCACAA
AGTCAGGGGGTAGATGTTATTGTGACAGACCACCACAGTTTGCCAGTAGAGCTACCAGAAGCATTTGCGATTGTGCACCC
AGAACACCCAAAAGCTGATTATCCGTTCAAATATCTGGCTGGCTGTGGCGTTGCTTTCAAAGTCGCTTGTGCTTTGCTAG
AATCTATTCCGACAGAAATGCTTGATCTGGTGGCAATTGGAACAATTGCTGATATGGTGAGTTTGACTGATGAAAACCGT
ATTATGGTTAAAGTCGGACTTGAAATTCTGAAACAAACCGAACGCATTGGTCTGATTGAATTGATGAAAGTCTCAGACGT
GGATATGGCAGATGTCAATGAAGAAACGGTTGGTTTCAAAATTGCTCCGCAGCTAAATGCACTTGGTCGCCTAGATGATC
CAAATCCTGCTGTCGAATTATTGACTGGCTTTGATGATGAAGAAGCGCATAACATTGCCAAGATGATTAATGCTAAAAAC
GAAGAACGCAAGGAAATTGTTCAGAAAATCTTCGATGAAGCCATGAGCATGGTTGATTTGGAAAAGCCAGTGCAAGTATT
GGCTAAAGAAGGCTGGCACCCAGGCGTTTTAGGAATTGTCGCAGGGCGTATTTCAGAACAAATCGCGCAGCCAGTGATTG
TTTTAAATATCGAAGATGGGCTTGCTAAAGGTTCAGCCAGAAGTATTGAGACCATTAATATTTTCCATGCTCTTGATGAG
CACCGTGACTTATTTGAAGCCTTTGGTGGACATGCTGGTGCTGCTGGAATGACCTTGGTGACTGAAAATCTTGATAAATT
ATTACAAGTTTTGTGTGATTATATTGCTGAAAATGATATTGACTTATCACAGAAAAAAGAGCTTGTTATTGATGAGGTTC
TGGAGCTATCAGAGATTGACCTTGATACCGTGCAAAGTCTTGAAAAATTGGCACCATTTGGCATGGACAATCCAAAACCA
GTTTTTGAAATTAAAGATTTTGTGGTGAAACAAGCACGCACTATGGGGCAAAATGGTGCGCATTTAAAATTAAAAATTGC
CCAAGGCACAACGGCAATTGATCTTGTTGCCTTTAATCAAGGACATCTGGCGCAGGAATTTCAACAAGCACAAAATCTTT
GCCTTGCAACGACCTTATCGATTAATAAGTGGAATGGGCAAATGACTGTTCAATTAATGTTGGAAGATGCGCATGTTGAG
GGTGTTCAGTTGATTGATATTCGCTCCAAAACGGCTAGCCTTCCAGAACGTGTTCCAGTATTGGCTGAAGACAGCACGGC
AAATGAAGTTGTGGTGTTGGACATTCCTGATAAGGCTGAAGAATTGAAATCACTCTTTGTCGGTCGTCATTTTGAAGCCG
TTTATTTTAAAAATCATATTAAACGTGCTTATTATCTGACAGGCTATGGCACGCGTGACCAATTTGCTAAGCTTTATAAA
ACTATTTATCAATTCCCAGAATTTGATGTCCGCTACAAGTTGAAAGATTTGAGTCAATACCTTAACATCCCTGATATTTT
GCTCATTAAAATGATTCAGATTTTTGATGAATTAGATTTTGTAACTATCACAGATGGTGTTATGGTCGTCAACAAAGATG
CCCAGAAACGTAGTATTTCAGAAAGCCAAATCTACCAAGACCTCAAACAACAAGTTGAATTTCAAGAGCTAATGGCACTC
GGCACCCCACAAGAAATTTACGATTGGCTGACTGATGAAGATGACTAA

Domains

Predicted by InterProScan.

DHH family (DHH)

(83-228)

RecJ OB domain (RecJ_OB)

(450-556)

DHHA1 domain (DHHA1)

(317-434)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(583-730)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2G3NWI2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

66.757

100

0.672
  recJ Bacillus subtilis subsp. subtilis str. 168

39.097

100

0.412