Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NYR91_RS12855 Genome accession   NZ_CP103784
Coordinates   2499615..2500025 (-) Length   136 a.a.
NCBI ID   WP_014477402.1    Uniprot ID   -
Organism   Bacillus subtilis strain RO-NN-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2494615..2505025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR91_RS12825 (NYR91_12825) - 2494703..2494843 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  NYR91_RS12830 (NYR91_12830) yqeF 2495149..2495880 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  NYR91_RS12835 (NYR91_12835) cwlH 2496131..2496883 (-) 753 WP_014477398.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  NYR91_RS12840 (NYR91_12840) yqeD 2497070..2497696 (+) 627 WP_014477399.1 TVP38/TMEM64 family protein -
  NYR91_RS12845 (NYR91_12845) gnd 2497715..2498608 (-) 894 WP_014477400.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NYR91_RS12850 (NYR91_12850) yqeB 2498860..2499582 (+) 723 WP_014477401.1 hypothetical protein -
  NYR91_RS12855 (NYR91_12855) nucA/comI 2499615..2500025 (-) 411 WP_014477402.1 sporulation-specific Dnase NucB Machinery gene
  NYR91_RS12860 (NYR91_12860) sigK 2500221..2500949 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  NYR91_RS12865 (NYR91_12865) - 2500949..2501065 (+) 117 WP_157863915.1 hypothetical protein -
  NYR91_RS12870 (NYR91_12870) - 2501031..2501228 (-) 198 Protein_2494 recombinase family protein -
  NYR91_RS12875 (NYR91_12875) - 2501186..2501364 (-) 179 Protein_2495 hypothetical protein -
  NYR91_RS20630 - 2501886..2502276 (+) 391 Protein_2496 SMI1/KNR4 family protein -
  NYR91_RS12885 (NYR91_12885) - 2502321..2502866 (-) 546 WP_014112744.1 TetR/AcrR family transcriptional regulator -
  NYR91_RS12890 (NYR91_12890) - 2503091..2504215 (+) 1125 WP_014477406.1 alpha/beta hydrolase -
  NYR91_RS12895 (NYR91_12895) - 2504385..2504660 (+) 276 WP_014477407.1 HPr family phosphocarrier protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14977.95 Da        Isoelectric Point: 5.1853

>NTDB_id=725030 NYR91_RS12855 WP_014477402.1 2499615..2500025(-) (nucA/comI) [Bacillus subtilis strain RO-NN-1]
MKKWLAGLFLAAAVLLCLMVPQQIQGASSYDRVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=725030 NYR91_RS12855 WP_014477402.1 2499615..2500025(-) (nucA/comI) [Bacillus subtilis strain RO-NN-1]
ATGAAAAAATGGTTGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
TTCTTCGTATGACAGAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCAGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGTTATCCTGACGGCACCAGAGTGCTATTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529