Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   M5T12_RS23035 Genome accession   NZ_CP103751
Coordinates   4633308..4634741 (+) Length   477 a.a.
NCBI ID   WP_032659700.1    Uniprot ID   -
Organism   Enterobacter asburiae strain 2497     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4628308..4639741
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T12_RS23005 (M5T12_23005) - 4628875..4629852 (+) 978 WP_023309354.1 calcium/sodium antiporter -
  M5T12_RS23010 (M5T12_23010) kdsD 4629866..4630852 (+) 987 WP_023309355.1 arabinose-5-phosphate isomerase KdsD -
  M5T12_RS23015 (M5T12_23015) kdsC 4630867..4631433 (+) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  M5T12_RS23020 (M5T12_23020) lptC 4631430..4632005 (+) 576 WP_023309357.1 LPS export ABC transporter periplasmic protein LptC -
  M5T12_RS23025 (M5T12_23025) lptA 4631974..4632528 (+) 555 WP_023309358.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  M5T12_RS23030 (M5T12_23030) lptB 4632535..4633260 (+) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  M5T12_RS23035 (M5T12_23035) rpoN/rpoN1 4633308..4634741 (+) 1434 WP_032659700.1 RNA polymerase factor sigma-54 Machinery gene
  M5T12_RS23040 (M5T12_23040) hpf 4634764..4635051 (+) 288 WP_008501444.1 ribosome hibernation promoting factor -
  M5T12_RS23045 (M5T12_23045) ptsN 4635134..4635625 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  M5T12_RS23050 (M5T12_23050) rapZ 4635671..4636525 (+) 855 WP_014885397.1 RNase adapter RapZ -
  M5T12_RS23055 (M5T12_23055) npr 4636522..4636794 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  M5T12_RS23060 (M5T12_23060) mtgA 4636834..4637559 (-) 726 WP_045355397.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  M5T12_RS23065 (M5T12_23065) elbB 4637556..4638209 (-) 654 WP_045355427.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54169.89 Da        Isoelectric Point: 4.3149

>NTDB_id=724502 M5T12_RS23035 WP_032659700.1 4633308..4634741(+) (rpoN/rpoN1) [Enterobacter asburiae strain 2497]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=724502 M5T12_RS23035 WP_032659700.1 4633308..4634741(+) (rpoN/rpoN1) [Enterobacter asburiae strain 2497]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
GCTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTACCAGGGTGAGACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACGTCCATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATTCTGGAAAGCATGGGCGACGACGAGATTGAGCTTGAGGAAGTCGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGCTCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCCAACCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAGTACGTCATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAATTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48