Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   EKM74_RS07040 Genome accession   NZ_AP019306
Coordinates   1269339..1270967 (+) Length   542 a.a.
NCBI ID   WP_001186602.1    Uniprot ID   Q9XB94
Organism   Staphylococcus aureus strain TUM9463     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 1267968..1310014 1269339..1270967 within 0


Gene organization within MGE regions


Location: 1267968..1310014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKM74_RS07035 ccrA 1267968..1269317 (+) 1350 WP_000815646.1 cassette chromosome recombinase CcrA Machinery gene
  EKM74_RS07040 ccrB 1269339..1270967 (+) 1629 WP_001186602.1 cassette chromosome recombinase CcrB Machinery gene
  EKM74_RS07045 - 1271485..1271835 (+) 351 WP_000859155.1 SAUGI family uracil-DNA glycosylase inhibitor -
  EKM74_RS07050 - 1271922..1272233 (+) 312 WP_000700853.1 DUF960 family protein -
  EKM74_RS07055 - 1272251..1272757 (+) 507 WP_001092169.1 DUF1643 domain-containing protein -
  EKM74_RS07060 - 1272778..1273095 (+) 318 WP_000659050.1 JAB domain-containing protein -
  EKM74_RS07065 - 1273214..1274299 (+) 1086 WP_000868132.1 tyrosine-type recombinase/integrase -
  EKM74_RS07070 - 1274296..1276188 (+) 1893 WP_001557544.1 site-specific integrase -
  EKM74_RS07075 - 1276195..1276572 (+) 378 WP_000361059.1 DUF6262 family protein -
  EKM74_RS07080 - 1276723..1277505 (+) 783 WP_000067268.1 aminoglycoside nucleotidyltransferase ANT(9)-Ia -
  EKM74_RS07085 erm(A) 1277631..1278362 (-) 732 WP_001072201.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) -
  EKM74_RS07090 - 1278436..1279395 (-) 960 WP_002303350.1 IS30 family transposase -
  EKM74_RS16620 - 1279467..1279655 (-) 189 WP_420066751.1 JAB domain-containing protein -
  EKM74_RS07100 - 1279887..1280549 (-) 663 WP_001092058.1 class I SAM-dependent methyltransferase -
  EKM74_RS16625 - 1280941..1281027 (+) 87 WP_193996833.1 erythromycin resistance leader peptide -
  EKM74_RS07115 - 1281091..1282050 (+) 960 WP_002303350.1 IS30 family transposase -
  EKM74_RS07120 - 1282061..1282648 (-) 588 Protein_1308 sulfite exporter TauE/SafE family protein -
  EKM74_RS07125 cstR 1282648..1282908 (-) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  EKM74_RS07130 - 1283044..1284108 (+) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  EKM74_RS07135 cstB 1284141..1285475 (+) 1335 Protein_1311 persulfide dioxygenase-sulfurtransferase CstB -
  EKM74_RS07145 - 1285589..1286698 (-) 1110 WP_232516977.1 ROK family transcriptional regulator -
  EKM74_RS07150 psm-mec 1287053..1287121 (+) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  EKM74_RS07155 mecI 1287206..1287577 (-) 372 WP_000369214.1 mecA-type methicillin resistance repressor MecI -
  EKM74_RS07160 mecR1 1287577..1289334 (-) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  EKM74_RS07165 mecA 1289431..1291440 (+) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  EKM74_RS07170 - 1291486..1291914 (-) 429 WP_000872606.1 MaoC family dehydratase -
  EKM74_RS07175 - 1292011..1292754 (-) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  EKM74_RS07185 - 1293391..1293780 (-) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  EKM74_RS07190 - 1293816..1294490 (+) 675 WP_125571304.1 IS6 family transposase -
  EKM74_RS07195 - 1294522..1294761 (-) 240 WP_001282728.1 hypothetical protein -
  EKM74_RS07205 - 1295100..1296272 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  EKM74_RS07210 - 1296508..1297299 (-) 792 Protein_1323 MrcB family domain-containing protein -
  EKM74_RS07215 - 1297309..1298268 (-) 960 WP_002303350.1 IS30 family transposase -
  EKM74_RS07220 - 1298363..1298830 (-) 468 Protein_1325 MrcB family domain-containing protein -
  EKM74_RS07225 - 1299233..1299781 (-) 549 WP_083553773.1 hypothetical protein -
  EKM74_RS07230 - 1299774..1300748 (-) 975 WP_125571308.1 nucleotidyltransferase -
  EKM74_RS07235 - 1300933..1302447 (+) 1515 WP_083553771.1 type I restriction-modification system subunit M -
  EKM74_RS07240 - 1302437..1303570 (+) 1134 WP_125571310.1 restriction endonuclease subunit S -
  EKM74_RS07245 - 1303554..1306673 (+) 3120 WP_125571312.1 type I restriction endonuclease subunit R -
  EKM74_RS07250 - 1306747..1306968 (-) 222 WP_001015190.1 hypothetical protein -
  EKM74_RS07255 - 1306982..1307485 (-) 504 WP_001092167.1 DUF1643 domain-containing protein -
  EKM74_RS07260 - 1307503..1307814 (-) 312 WP_001024801.1 DUF960 domain-containing protein -
  EKM74_RS07270 - 1307908..1308246 (-) 339 WP_125571314.1 SAUGI family uracil-DNA glycosylase inhibitor -
  EKM74_RS07275 ccrC 1308335..1310014 (-) 1680 WP_103329647.1 cassette chromosome recombinase CcrC -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62664.84 Da        Isoelectric Point: 9.8600

>NTDB_id=72308 EKM74_RS07040 WP_001186602.1 1269339..1270967(+) (ccrB) [Staphylococcus aureus strain TUM9463]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRILKDAKEGYIDCVM
VYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPNSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDIAYKQQQYD
EVQIKLNNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNEDKPLFDAKEISKLLQHIFHDIKHIEKSRLK
ALYLSVIDRIDIKKDGNHKKQFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=72308 EKM74_RS07040 WP_001186602.1 1269339..1270967(+) (ccrB) [Staphylococcus aureus strain TUM9463]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTATCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGACGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAAGTTAAAGATATATACGCTGACCGTGGTA
TTTCAGGTAAATCTATGAATCGACCTGAGCTCCAACGTATATTGAAGGATGCGAAAGAAGGCTATATCGACTGTGTTATG
GTCTACAAAACAAACCGATTAGCTCGTAATACATCTGATCTTCTCAAAATTGTCGAAGATTTACACAAACAAAATGTCGA
ATTTTTCAGTTTATCAGAGCGTATGGAAGTCAATACTTCATCGGGTAAGCTCATGTTACAAATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCGAGAATGTATTTATGGGTCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGCAAT
TTGCCGCTGGGCTATGACAAAATACCTAATAGTAAACATGAACTGATGATTAATCAACATGAAGCTAATATTGTGAAATA
TATATTCGAGTCCTATGCCAAAGGCCATGGCTATCGTAAAATAGCCAATGCATTAAATCACAAAGGCTATGTCACTAAAA
AGGGTAAACCTTTTAGTATTAGTTCTATCACATATATATTAGCTAACCCATTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTTAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAAAAACAAGTCAGTCAAAAACCCCAAGTCCATGGCAAAGGAACGA
ATCTGCTTACAGGCATTATTCACTGTCCCCAATGTGGCGCACCTATGGCAGCAAGCAATACCACGAATACACTTAAAGAC
GGGACTAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGTTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTGATGTGATTGAAGATTATGTGATGAAGCAAATACTTGAAATAGTCAAAAGTGATAAAGTCATTCAACGCGTAG
TAACACACGTCAATCAAGAAAATCAAGTTGATGGCGCTGCACTTCATCACGATATTGCTTATAAGCAACAACAATATGAT
GAAGTACAAATCAAACTAAATAACTTGATTAAAACCATCGAGGATAATCCGGACTTAACATCAGTAATCAGACCAAGTAT
TCAAAAATATGAAAAGCAACTCAATGACATTACGAATCAAATCAACCAACTCAAAAATCAACAAAATGAAGATAAGCCTT
TATTTGATGCCAAAGAAATCAGTAAACTATTACAACACATCTTTCATGATATTAAGCACATAGAAAAATCTCGACTCAAA
GCATTGTATCTATCAGTGATTGATCGCATTGATATTAAAAAAGATGGTAATCATAAAAAACAATTCTATGTCACACTCAA
ACTTAATAACGAAATCATAAAACAACTTTTCAATAATAAACAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTGTTTT
TGCCCCAAACACTATATCTTACTATTTAG

Domains

Predicted by InterproScan.

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9XB94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

100

100

1

Multiple sequence alignment