Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   M5T15_RS19915 Genome accession   NZ_CP103494
Coordinates   4155064..4156497 (+) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain 70     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4150064..4161497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T15_RS19885 (M5T15_19885) - 4150631..4151608 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  M5T15_RS19890 (M5T15_19890) kdsD 4151622..4152608 (+) 987 WP_045141579.1 arabinose-5-phosphate isomerase KdsD -
  M5T15_RS19895 (M5T15_19895) kdsC 4152623..4153189 (+) 567 WP_045141580.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  M5T15_RS19900 (M5T15_19900) lptC 4153186..4153761 (+) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  M5T15_RS19905 (M5T15_19905) lptA 4153730..4154284 (+) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  M5T15_RS19910 (M5T15_19910) lptB 4154291..4155016 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  M5T15_RS19915 (M5T15_19915) rpoN/rpoN1 4155064..4156497 (+) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  M5T15_RS19920 (M5T15_19920) hpf 4156520..4156807 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  M5T15_RS19925 (M5T15_19925) ptsN 4156890..4157381 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  M5T15_RS19930 (M5T15_19930) rapZ 4157427..4158281 (+) 855 WP_003861870.1 RNase adapter RapZ -
  M5T15_RS19935 (M5T15_19935) npr 4158278..4158550 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  M5T15_RS19940 (M5T15_19940) mtgA 4158590..4159315 (-) 726 WP_045141581.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  M5T15_RS19945 (M5T15_19945) elbB 4159312..4159965 (-) 654 WP_045141582.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=723044 M5T15_RS19915 WP_003861864.1 4155064..4156497(+) (rpoN/rpoN1) [Enterobacter hormaechei strain 70]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=723044 M5T15_RS19915 WP_003861864.1 4155064..4156497(+) (rpoN/rpoN1) [Enterobacter hormaechei strain 70]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGATTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATTTACACCGCGGGTACCCCTTCCGGGACACGTGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACCCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTTGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTTCTGAAACGCGTTCAGCGTTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGCGACTGTCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCACGACTTCCGCACCCTGATGCGCGTCACACGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATCCCCGATGTGCTGGTGAGAAAACACAATGGCCGGTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCCCGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48