Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NX050_RS20085 Genome accession   NZ_CP103456
Coordinates   3751724..3752173 (+) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain PN176 (HK176)     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3746724..3757173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX050_RS20065 (NX050_20065) hxlR 3747938..3748300 (-) 363 WP_003246265.1 transcriptional activator HxlR -
  NX050_RS20070 (NX050_20070) hxlA 3748529..3749161 (+) 633 WP_014478804.1 3-hexulose-6-phosphate synthase -
  NX050_RS20075 (NX050_20075) hxlB 3749167..3749724 (+) 558 WP_014478803.1 6-phospho-3-hexuloisomerase -
  NX050_RS20080 (NX050_20080) tlpC 3749835..3751556 (+) 1722 Protein_3944 methyl-accepting chemotaxis protein TlpC -
  NX050_RS20085 (NX050_20085) nucA/comI 3751724..3752173 (+) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  NX050_RS20090 (NX050_20090) nin/comJ 3752200..3752598 (+) 399 WP_014478800.1 DNA-entry nuclease inhibitor Regulator
  NX050_RS20095 (NX050_20095) bglC 3752635..3754068 (-) 1434 WP_014478799.1 6-phospho-beta-glucosidase -
  NX050_RS20100 (NX050_20100) - 3754141..3754885 (-) 745 Protein_3948 AAA family ATPase -
  NX050_RS20105 (NX050_20105) - 3754904..3755224 (-) 321 WP_014478796.1 DUF3147 family protein -
  NX050_RS20110 (NX050_20110) - 3755212..3755634 (-) 423 WP_014478795.1 MarR family winged helix-turn-helix transcriptional regulator -
  NX050_RS20115 (NX050_20115) yckD 3755762..3756094 (-) 333 WP_014478794.1 YckD family protein -
  NX050_RS20120 (NX050_20120) yckC 3756176..3756631 (-) 456 WP_014478793.1 RDD family protein -
  NX050_RS20125 (NX050_20125) - 3756651..3756848 (-) 198 WP_121509395.1 hypothetical protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=722915 NX050_RS20085 WP_014475770.1 3751724..3752173(+) (nucA/comI) [Bacillus subtilis strain PN176 (HK176)]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=722915 NX050_RS20085 WP_014475770.1 3751724..3752173(+) (nucA/comI) [Bacillus subtilis strain PN176 (HK176)]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987