Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NRD65_RS18895 Genome accession   NZ_CP103409
Coordinates   3970622..3972055 (+) Length   477 a.a.
NCBI ID   WP_023295498.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain EHA64     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3965622..3977055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRD65_RS18865 (NRD65_18835) - 3966189..3967166 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  NRD65_RS18870 (NRD65_18840) kdsD 3967180..3968166 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  NRD65_RS18875 (NRD65_18845) kdsC 3968181..3968747 (+) 567 WP_017693909.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NRD65_RS18880 (NRD65_18850) lptC 3968744..3969319 (+) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  NRD65_RS18885 (NRD65_18855) lptA 3969288..3969842 (+) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NRD65_RS18890 (NRD65_18860) lptB 3969849..3970574 (+) 726 WP_023304493.1 LPS export ABC transporter ATP-binding protein -
  NRD65_RS18895 (NRD65_18865) rpoN/rpoN1 3970622..3972055 (+) 1434 WP_023295498.1 RNA polymerase factor sigma-54 Machinery gene
  NRD65_RS18900 (NRD65_18870) hpf 3972078..3972365 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  NRD65_RS18905 (NRD65_18875) ptsN 3972448..3972939 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NRD65_RS18910 (NRD65_18880) rapZ 3972985..3973839 (+) 855 WP_110292552.1 RNase adapter RapZ -
  NRD65_RS18915 (NRD65_18885) npr 3973836..3974108 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  NRD65_RS18920 (NRD65_18890) mtgA 3974148..3974873 (-) 726 WP_045348879.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  NRD65_RS18925 (NRD65_18895) elbB 3974870..3975523 (-) 654 WP_015571987.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54098.98 Da        Isoelectric Point: 4.3549

>NTDB_id=722588 NRD65_RS18895 WP_023295498.1 3970622..3972055(+) (rpoN/rpoN1) [Enterobacter hormaechei strain EHA64]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGNDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=722588 NRD65_RS18895 WP_023295498.1 3970622..3972055(+) (rpoN/rpoN1) [Enterobacter hormaechei strain EHA64]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATTTACACCGCGGGTACCCCTTCCGGGACACGTGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACCCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGTTGGACGAG
ATTCTTGAAAGCATCGGCAATGACGAGATTGAACTGGAAGAGATCGAAGCCGTTCTGAAACGCGTTCAGCGTTTCGACCC
GATAGGTGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCACGACTTCCGCACCCTGATGCGCGTCACACGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGGTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCAGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48