Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   PWK65_RS03695 Genome accession   NZ_CP118481
Coordinates   727306..729516 (+) Length   736 a.a.
NCBI ID   WP_002990110.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 20185322     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 722306..734516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWK65_RS03665 (PWK65_03620) - 722453..722629 (-) 177 WP_002984898.1 DUF3042 family protein -
  PWK65_RS03670 (PWK65_03625) miaA 722763..723662 (+) 900 WP_002990117.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  PWK65_RS03675 (PWK65_03630) hflX 723735..724973 (+) 1239 WP_002990116.1 GTPase HflX -
  PWK65_RS03680 (PWK65_03635) - 724966..725601 (+) 636 WP_002990114.1 cystathionine beta-lyase -
  PWK65_RS03685 (PWK65_03640) rnz 725616..726545 (+) 930 WP_002984894.1 ribonuclease Z -
  PWK65_RS03690 (PWK65_03645) - 726545..727309 (+) 765 WP_002990111.1 SDR family oxidoreductase -
  PWK65_RS03695 (PWK65_03650) recJ 727306..729516 (+) 2211 WP_002990110.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  PWK65_RS03700 (PWK65_03655) - 729667..730185 (+) 519 WP_002990109.1 adenine phosphoribosyltransferase -
  PWK65_RS03705 (PWK65_03660) - 730266..730949 (+) 684 WP_002984890.1 DnaD domain-containing protein -
  PWK65_RS03710 (PWK65_03665) nth 730946..731602 (+) 657 WP_002990106.1 endonuclease III -
  PWK65_RS03715 (PWK65_03670) - 731674..732360 (+) 687 WP_002990104.1 tRNA (adenine(22)-N(1))-methyltransferase TrmK -
  PWK65_RS03720 (PWK65_03675) - 732350..733138 (+) 789 WP_002984887.1 Nif3-like dinuclear metal center hexameric protein -
  PWK65_RS03725 (PWK65_03680) - 733173..734279 (+) 1107 WP_002990103.1 FAD-binding oxidoreductase -

Sequence

Protein


Download         Length: 736 a.a.        Molecular weight: 82565.42 Da        Isoelectric Point: 4.7844

>NTDB_id=722474 PWK65_RS03695 WP_002990110.1 727306..729516(+) (recJ) [Streptococcus pyogenes strain 20185322]
MIKSKYSWKIKDKKPDDGFFKLAKTKGLTQTAAQLIYDRGIRTEEALDEFLTADLSQLHDPYLLHDMAKAVPRIRQAIEE
GERILIYGDYDADGMTSASIVKETLDMMGAEPLVYLPNRFTDGYGPNQSVYKYFIEQEAVSLIITVDNGVAGHEAIRYAQ
EQEVDVIVTDHHSLPEELPEAFAIIHPEHPDADYPFKHLAGCGVAFKLATALLESLPTDCLDLVAIGTIADMVSLTGENR
VLVKNGLAMLKHTERVGLQELMSLSPIDLEHFNEDAIGFQIAPQLNALGRLDDPNPAIELLTGFDDQEAQAIALMIKKKN
EERKALVQDIFDQAMAMVDPQKPVQVLAQAGWHPGVLGIVAGRIMETIGQTVVVLTIDNGFAKGSARSLEAINIFEALNG
KRELFTAFGGHAGAAGMTLPVDNLEALSDFLCQFVIERGLDQTAKNTLTIDERLSLDDLSLDILKSLDKLAPYGMDHQKP
VFYVKDIRVSQARTIGQDQSHLKFKVSQGKASFDVLAFGQGSQLQEFRQATGLELAVTLSVNHWNGNTSLQFMLVDARVD
GVQLLDLRTKTAKVPEGIPTIEEDPNARVILINDIPEDFKTWRNQFVHKDFDAIYFKNQMKHPYYLTGFGSREQFAKLYK
TIYQFPEFDLRHKLTELSHYLNIEKLLLIKLIQIFEELSFVTIDDGLMTVNPQAQKREISESHIYQDLKELVKFQEIMAL
ASPKEMYDYLVKSNDQ

Nucleotide


Download         Length: 2211 bp        

>NTDB_id=722474 PWK65_RS03695 WP_002990110.1 727306..729516(+) (recJ) [Streptococcus pyogenes strain 20185322]
ATGATTAAATCCAAATATAGCTGGAAAATAAAAGACAAGAAGCCAGATGATGGCTTCTTTAAGCTAGCAAAGACAAAAGG
TCTAACCCAGACCGCCGCTCAGTTGATTTATGATCGAGGGATAAGGACTGAAGAAGCCTTAGATGAGTTTTTAACAGCGG
ACTTGTCTCAACTGCATGATCCTTACTTGCTACATGACATGGCAAAAGCTGTTCCAAGAATTCGTCAAGCTATTGAAGAG
GGCGAACGAATTCTCATTTACGGCGACTATGATGCTGATGGCATGACGTCGGCTTCTATTGTCAAAGAAACCCTAGACAT
GATGGGAGCAGAGCCTTTGGTTTATCTTCCCAACCGCTTTACAGATGGCTATGGCCCCAACCAGAGCGTCTATAAATATT
TTATTGAGCAGGAAGCTGTGTCCCTAATTATCACGGTTGATAATGGCGTGGCCGGACATGAAGCGATTAGATATGCTCAA
GAACAGGAAGTTGATGTCATTGTAACTGACCATCATAGCCTGCCTGAAGAATTGCCAGAAGCCTTTGCCATCATTCATCC
TGAGCATCCTGATGCTGATTATCCCTTTAAACACTTAGCTGGCTGTGGCGTTGCCTTTAAATTGGCAACAGCTTTGCTAG
AAAGTTTACCAACAGACTGTCTAGACTTGGTAGCTATTGGAACCATTGCCGATATGGTAAGCTTGACAGGTGAAAACCGT
GTTTTAGTCAAAAATGGTCTGGCCATGCTCAAACACACCGAAAGAGTGGGCTTGCAGGAGTTGATGTCCTTGTCTCCTAT
TGATTTGGAACATTTTAATGAAGACGCCATTGGCTTTCAGATTGCTCCGCAATTAAATGCTTTAGGGCGCTTAGATGACC
CTAATCCAGCCATTGAGCTACTGACGGGCTTTGATGACCAAGAAGCTCAAGCGATTGCCTTGATGATTAAGAAAAAAAAT
GAAGAGCGTAAGGCCTTAGTCCAAGACATTTTTGATCAAGCGATGGCTATGGTTGATCCTCAAAAACCTGTTCAAGTCTT
GGCACAAGCTGGCTGGCATCCTGGTGTTCTTGGGATTGTCGCTGGTCGTATCATGGAAACTATTGGACAAACGGTCGTCG
TTTTGACCATTGATAATGGCTTTGCAAAAGGCTCGGCCAGAAGTTTAGAAGCTATTAATATTTTTGAAGCCTTGAATGGC
AAGCGTGAGTTATTTACAGCCTTTGGGGGACATGCTGGTGCAGCAGGAATGACCCTGCCCGTTGACAACTTGGAAGCTCT
TTCAGACTTTCTTTGTCAGTTTGTGATAGAACGTGGCTTAGATCAGACTGCCAAAAATACGTTAACTATTGATGAAAGAC
TGTCTTTGGACGACCTTAGCCTTGATATTTTAAAGAGTTTAGATAAATTAGCCCCTTATGGGATGGATCATCAAAAGCCG
GTCTTTTATGTCAAGGACATAAGGGTTAGTCAAGCGCGTACCATAGGGCAAGACCAGAGCCATCTTAAATTTAAAGTCAG
TCAGGGCAAAGCTTCCTTTGACGTACTGGCTTTTGGTCAAGGAAGTCAACTGCAAGAGTTCCGACAAGCTACTGGTTTAG
AATTAGCAGTTACCTTATCTGTCAACCATTGGAATGGGAACACGAGTCTCCAATTCATGTTAGTAGATGCTCGTGTTGAC
GGAGTGCAACTTCTTGACTTAAGGACAAAAACAGCCAAAGTTCCTGAAGGTATTCCGACTATTGAAGAAGACCCAAATGC
CAGAGTCATTCTGATTAATGACATCCCTGAAGATTTCAAAACCTGGCGCAACCAGTTTGTTCACAAGGATTTTGACGCCA
TCTATTTTAAAAATCAGATGAAACATCCTTATTATTTAACAGGATTTGGCAGCAGAGAGCAGTTTGCCAAGCTCTACAAA
ACCATCTACCAGTTTCCTGAATTTGATTTACGGCATAAATTGACAGAGTTGAGTCACTATTTAAACATAGAAAAACTTTT
ACTGATTAAGTTGATTCAAATTTTTGAAGAGTTGTCTTTTGTGACGATTGACGATGGGCTGATGACGGTTAACCCCCAAG
CGCAAAAACGAGAGATTTCAGAGAGCCACATTTACCAAGACTTAAAAGAATTGGTGAAATTTCAAGAAATCATGGCTTTG
GCTAGCCCGAAAGAGATGTATGATTATTTGGTGAAATCAAACGATCAATAA

Domains

Predicted by InterProScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(588-729)

DHHA1 domain (DHHA1)

(317-435)

RecJ OB domain (RecJ_OB)

(450-556)

DHH family (DHH)

(83-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

63.008

100

0.632
  recJ Bacillus subtilis subsp. subtilis str. 168

34.866

100

0.371