Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   NX820_RS08040 Genome accession   NZ_CP103391
Coordinates   1657850..1659655 (+) Length   601 a.a.
NCBI ID   WP_015473911.1    Uniprot ID   M5AFG0
Organism   Levilactobacillus brevis strain PL102     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1652850..1664655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX820_RS08015 (NX820_08015) - 1653039..1653773 (+) 735 WP_011668206.1 ABC transporter ATP-binding protein -
  NX820_RS08020 (NX820_08020) - 1653770..1654996 (+) 1227 WP_011668205.1 ABC transporter permease -
  NX820_RS08025 (NX820_08025) - 1655058..1655849 (+) 792 WP_011668204.1 phosphotransferase family protein -
  NX820_RS08030 (NX820_08030) trmB 1655915..1656562 (+) 648 WP_015473913.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  NX820_RS08035 (NX820_08035) - 1656689..1657783 (+) 1095 WP_015473912.1 competence protein CoiA -
  NX820_RS08040 (NX820_08040) pepF 1657850..1659655 (+) 1806 WP_015473911.1 oligoendopeptidase F Regulator
  NX820_RS08045 (NX820_08045) - 1659709..1660572 (-) 864 WP_085769115.1 DegV family protein -
  NX820_RS08050 (NX820_08050) - 1660734..1661372 (-) 639 WP_011668199.1 ClpXP adapter SpxH family protein -
  NX820_RS08055 (NX820_08055) - 1661686..1662345 (+) 660 WP_011668198.1 GTP pyrophosphokinase family protein -
  NX820_RS08060 (NX820_08060) - 1662347..1663150 (+) 804 WP_021740943.1 NAD kinase -
  NX820_RS08065 (NX820_08065) - 1663147..1664043 (+) 897 WP_087609511.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 601 a.a.        Molecular weight: 67135.10 Da        Isoelectric Point: 4.7804

>NTDB_id=722456 NX820_RS08040 WP_015473911.1 1657850..1659655(+) (pepF) [Levilactobacillus brevis strain PL102]
MKQIPKRSEVPTALTWDLTTIFPDEAAFKAAIAAIKTQTTVVAGLKGQLAQSGAALYRVTTAVFDLNRQLERVYVYASLN
NDQDTGNAAAQALMGQAESLVATVGAATAWFEPEVLALSADQLQTLIDNDPRLADYQHVFDVLGQQRAHTLSVAEEKLLA
GASDIFGASAKTYSVLSDADLKFPVVQDEAGNDVRLSEGLYGVLLQSTTPRVRQQAFEALYSVYQQFRHTFAATLASEVK
THNFSAETRHYASARAAAMSRNNVPAVVYDTLVETVNDHLDSLHRYVNLRKEILDLPQLHMYDLYTPITGEPSLKYTYQE
AQEMALKALAVLGPDYTANVQKMFDGRAIDVVENQGKRTGAYSGGMYDTKPYILLNWQDSLESLFTLVHEMGHSMHSHYT
RTNQPYQYGDYSIFVAEIASTTNENLLTDYLLKTQTDPKVRAYVLNHYLDGFKGTVYRQTQFAEFEDYIHQQDAAGETLT
ADFMSDFYGKLNQRYYGDGVISDPQIADEWTRIPHFYYDYYVYQYATGFAAATTLSQRILSGDEAKRDAYLAYLKAGSSA
LPLDVMKQAGVDMTQPGYLQTAFATFDERLAEFTQLAHELN

Nucleotide


Download         Length: 1806 bp        

>NTDB_id=722456 NX820_RS08040 WP_015473911.1 1657850..1659655(+) (pepF) [Levilactobacillus brevis strain PL102]
GTGAAACAAATTCCGAAACGATCAGAGGTTCCCACGGCACTGACTTGGGACCTGACAACTATTTTTCCCGACGAGGCAGC
TTTTAAGGCCGCAATTGCTGCAATTAAAACGCAAACAACGGTCGTGGCCGGTTTGAAGGGACAATTGGCCCAAAGTGGGG
CCGCGTTATATCGTGTCACGACGGCGGTATTTGATTTGAATCGCCAGTTGGAGCGGGTATATGTCTATGCGTCTTTAAAC
AATGATCAGGATACCGGCAATGCTGCAGCCCAGGCATTAATGGGGCAGGCCGAGAGCTTGGTGGCAACTGTGGGTGCCGC
CACGGCTTGGTTTGAACCTGAGGTATTGGCCTTGTCAGCGGATCAGCTGCAAACGTTGATTGATAACGACCCCCGGCTAG
CAGATTATCAGCATGTGTTTGACGTATTGGGCCAGCAGCGGGCACATACGCTGTCAGTGGCGGAAGAAAAATTGCTAGCG
GGTGCTAGTGATATCTTTGGTGCATCTGCTAAAACTTACAGCGTGCTGAGTGATGCTGATCTAAAGTTTCCAGTTGTTCA
AGACGAAGCCGGCAATGATGTGCGACTGTCAGAAGGACTGTATGGTGTTTTGCTACAATCCACAACACCACGGGTGCGGC
AGCAGGCTTTTGAAGCATTATATTCGGTTTATCAACAATTCCGACATACCTTTGCTGCAACCTTAGCGAGCGAAGTCAAA
ACGCATAATTTTAGTGCTGAGACGCGTCACTATGCGAGTGCCAGAGCGGCAGCGATGAGTCGCAACAATGTGCCAGCGGT
GGTCTATGATACCTTGGTGGAAACAGTCAATGATCATTTAGACTCACTGCATCGCTATGTTAATTTGCGCAAAGAGATTT
TGGACTTGCCACAACTGCACATGTACGACCTGTATACGCCAATTACGGGTGAACCCAGCTTGAAATATACCTACCAAGAA
GCACAAGAGATGGCGTTAAAGGCACTAGCAGTCTTGGGACCGGATTACACTGCCAATGTTCAAAAGATGTTTGATGGCCG
GGCAATTGATGTGGTTGAAAATCAGGGTAAACGGACGGGCGCTTACTCAGGCGGGATGTATGATACTAAACCGTATATTT
TGTTGAATTGGCAAGATAGCTTGGAAAGCTTGTTCACGTTGGTTCATGAGATGGGACACAGCATGCATAGCCATTACACT
CGGACAAATCAACCGTACCAGTATGGTGATTATTCAATCTTTGTTGCGGAAATTGCCTCGACAACGAATGAAAACTTGCT
GACGGACTACCTGTTGAAAACCCAGACTGACCCCAAGGTGCGAGCTTATGTATTAAATCATTATCTGGATGGCTTTAAAG
GCACGGTATACCGCCAGACGCAGTTCGCAGAATTTGAGGACTACATTCACCAACAGGATGCCGCTGGCGAAACTCTGACA
GCTGACTTCATGAGTGATTTCTACGGCAAACTTAATCAGCGTTATTACGGTGATGGCGTGATTTCCGATCCACAAATCGC
TGATGAATGGACCCGAATTCCACATTTCTACTATGACTACTATGTGTACCAGTACGCAACCGGGTTTGCGGCGGCCACGA
CGTTATCGCAACGAATTTTGAGTGGCGACGAGGCAAAGCGGGATGCCTACTTGGCCTATCTAAAGGCGGGAAGTTCTGCC
TTACCACTTGATGTGATGAAACAAGCGGGCGTCGATATGACGCAACCGGGCTATTTGCAAACGGCTTTTGCAACGTTCGA
CGAGCGCTTGGCAGAATTCACGCAATTGGCGCATGAATTAAACTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(114-183)

Peptidase family M3 (Peptidase_M3)

(204-584)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M5AFG0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

49.831

98.17

0.489