Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PVE41_RS10580 Genome accession   NZ_CP118438
Coordinates   2184555..2186018 (-) Length   487 a.a.
NCBI ID   WP_011078770.1    Uniprot ID   A0A3Q0L2E8
Organism   Vibrio vulnificus strain 1908-10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2179555..2191018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVE41_RS10550 (PVE41_10550) pmbA 2179626..2180969 (+) 1344 WP_011078776.1 metalloprotease PmbA -
  PVE41_RS10555 (PVE41_10555) mgtE 2181071..2182426 (-) 1356 WP_011078775.1 magnesium transporter -
  PVE41_RS10560 (PVE41_10560) - 2182641..2182916 (-) 276 WP_011078774.1 HPr family phosphocarrier protein -
  PVE41_RS10565 (PVE41_10565) rapZ 2182919..2183782 (-) 864 WP_206629243.1 RNase adapter RapZ -
  PVE41_RS10570 (PVE41_10570) ptsN 2183795..2184241 (-) 447 WP_011078772.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PVE41_RS10575 (PVE41_10575) hpf 2184244..2184531 (-) 288 WP_011078771.1 ribosome hibernation promoting factor -
  PVE41_RS10580 (PVE41_10580) rpoN/rpoN1 2184555..2186018 (-) 1464 WP_011078770.1 RNA polymerase factor sigma-54 Machinery gene
  PVE41_RS10585 (PVE41_10585) lptB 2186072..2186797 (-) 726 WP_011078769.1 LPS export ABC transporter ATP-binding protein -
  PVE41_RS10590 (PVE41_10590) lptA 2186797..2187291 (-) 495 WP_011078768.1 lipopolysaccharide transport periplasmic protein LptA -
  PVE41_RS10595 (PVE41_10595) lptC 2187272..2187835 (-) 564 WP_011078767.1 LPS export ABC transporter periplasmic protein LptC -
  PVE41_RS10600 (PVE41_10600) kdsC 2187832..2188389 (-) 558 WP_013572482.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PVE41_RS10605 (PVE41_10605) kdsD 2188389..2189360 (-) 972 WP_013572481.1 arabinose-5-phosphate isomerase KdsD -
  PVE41_RS10610 (PVE41_10610) - 2189382..2190347 (-) 966 WP_026050418.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 54653.65 Da        Isoelectric Point: 4.3786

>NTDB_id=722406 PVE41_RS10580 WP_011078770.1 2184555..2186018(-) (rpoN/rpoN1) [Vibrio vulnificus strain 1908-10]
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALESNPLLEVEEHQEEAQLNGEEKAINEGSDSDDFSVEIDI
PDSSDVIEKSEISSELEIDTTWDDVYSANSGNTGIALDDDMPVYQGETTETLHDYLQWQLDLTPFSDTDRMIAFAIIDAV
DDTGYLTISPEEILESLNSVDIELDEVEAVRKRIQQFDPLGVASQNLQECLLLQLATFPIETPWLAEAKMVLSHHIDALG
NRDYKLIVKEAKLKEEDLRETLKLIQQLDPRPGSKINAIEPEYVIPDVSVFKEHGKWSVAINPDSIPKLKVNQQYAQLGK
GSSADSQYIRSNLQEAKWLIKSLESRNETLLKVARCIVEHQHDFFEYGEEAMKPMVLNDVATAVEMHESTISRVTTQKFM
HTPRGIFELKYFFSSHVSTDSGGECSSTAIRALIKKLVTAENSAKPLSDSKIAALLADQGIQVARRTIAKYRESLGIAPS
SQRKRLL

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=722406 PVE41_RS10580 WP_011078770.1 2184555..2186018(-) (rpoN/rpoN1) [Vibrio vulnificus strain 1908-10]
ATGAAACCTTCATTACAACTCAAGCTAGGTCAACAGTTAGCCATGACGCCTCAGCTCCAGCAGGCCATACGTTTGCTGCA
GCTGTCCACCTTGGATTTACAGCAAGAAATCCAAGAAGCGCTGGAATCAAATCCCTTATTGGAAGTGGAAGAGCATCAAG
AAGAAGCGCAACTGAATGGCGAAGAGAAAGCGATCAATGAAGGTTCCGATTCTGATGATTTTTCTGTTGAGATCGACATT
CCCGACAGCTCTGATGTGATTGAAAAATCCGAAATCAGTAGCGAACTGGAAATCGACACCACCTGGGATGACGTTTACAG
TGCCAACTCTGGCAATACGGGCATCGCACTCGACGATGACATGCCGGTCTATCAAGGTGAAACGACGGAAACACTGCACG
ACTACTTACAGTGGCAACTGGATTTAACCCCGTTTAGCGATACTGACCGCATGATTGCCTTTGCCATCATTGATGCCGTC
GACGACACTGGCTACCTCACTATCAGCCCTGAAGAGATCTTAGAAAGCCTGAACAGCGTCGATATCGAACTCGACGAAGT
GGAAGCCGTACGCAAACGCATCCAACAGTTCGATCCCCTTGGTGTCGCGTCGCAAAACCTACAAGAATGCTTGCTGCTAC
AACTGGCCACATTTCCTATCGAGACCCCTTGGTTAGCCGAAGCCAAAATGGTCCTGAGCCATCACATTGATGCACTGGGC
AATCGCGACTACAAGCTGATCGTCAAAGAAGCGAAGCTTAAAGAAGAAGACCTACGTGAAACGCTCAAACTGATCCAGCA
ACTTGATCCAAGGCCTGGCAGTAAGATCAATGCCATCGAGCCTGAGTACGTGATTCCAGATGTATCCGTATTTAAAGAGC
ATGGCAAATGGAGTGTCGCGATCAATCCAGACAGCATTCCAAAATTAAAAGTAAATCAACAGTATGCGCAACTTGGTAAA
GGCAGCAGCGCCGATAGCCAGTACATTCGCAGCAATTTGCAAGAGGCGAAATGGTTAATTAAGAGCCTTGAGAGCAGAAA
CGAGACGCTGCTCAAAGTTGCACGTTGCATAGTTGAACATCAACATGATTTCTTCGAGTATGGGGAAGAAGCAATGAAAC
CTATGGTGCTAAATGATGTGGCAACCGCGGTTGAGATGCATGAATCCACCATCTCACGCGTGACAACCCAGAAGTTTATG
CACACGCCTAGAGGTATCTTTGAATTGAAATATTTCTTCTCTAGCCATGTTAGTACGGATAGTGGAGGAGAATGTTCATC
GACAGCAATTCGCGCACTCATTAAGAAGTTAGTCACTGCCGAAAACAGTGCGAAACCACTGAGTGATAGCAAAATTGCTG
CCTTACTGGCAGACCAAGGAATCCAGGTCGCACGGCGAACGATAGCTAAGTACCGCGAGTCCTTAGGCATTGCCCCATCT
AGTCAGCGAAAACGCCTACTTTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(327-485)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L2E8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

43.168

100

0.448