Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   PVE41_RS06960 Genome accession   NZ_CP118438
Coordinates   1431859..1433583 (-) Length   574 a.a.
NCBI ID   WP_011079397.1    Uniprot ID   A0A3Q0L3L1
Organism   Vibrio vulnificus strain 1908-10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1426859..1438583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVE41_RS06925 (PVE41_06925) - 1427239..1427928 (-) 690 WP_045622017.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PVE41_RS06930 (PVE41_06930) - 1427928..1428494 (-) 567 WP_061058936.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PVE41_RS06935 (PVE41_06935) - 1428494..1428976 (-) 483 WP_130268270.1 type II secretion system protein -
  PVE41_RS06940 (PVE41_06940) - 1429006..1429473 (-) 468 WP_101957357.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PVE41_RS06945 (PVE41_06945) - 1429507..1430085 (-) 579 WP_011079400.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PVE41_RS06950 (PVE41_06950) - 1430164..1430625 (-) 462 WP_108677230.1 MSHA biogenesis protein MshF -
  PVE41_RS06955 (PVE41_06955) - 1430629..1431849 (-) 1221 WP_011079398.1 type II secretion system F family protein -
  PVE41_RS06960 (PVE41_06960) pilF 1431859..1433583 (-) 1725 WP_011079397.1 GspE/PulE family protein Machinery gene
  PVE41_RS06965 (PVE41_06965) - 1433580..1434701 (-) 1122 WP_282500167.1 tetratricopeptide repeat protein -
  PVE41_RS06970 (PVE41_06970) - 1434698..1435546 (-) 849 WP_045622006.1 AAA family ATPase -
  PVE41_RS06975 (PVE41_06975) mshL 1435554..1437200 (-) 1647 WP_072615906.1 pilus (MSHA type) biogenesis protein MshL -
  PVE41_RS06980 (PVE41_06980) - 1437216..1437536 (-) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  PVE41_RS06985 (PVE41_06985) gspM 1437529..1438179 (-) 651 WP_011079392.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63672.03 Da        Isoelectric Point: 5.3226

>NTDB_id=722375 PVE41_RS06960 WP_011079397.1 1431859..1433583(-) (pilF) [Vibrio vulnificus strain 1908-10]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRSKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHGMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLDEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=722375 PVE41_RS06960 WP_011079397.1 1431859..1433583(-) (pilF) [Vibrio vulnificus strain 1908-10]
ATGAAAGTAAGATTAAGAAAGCGACTTGGTGACCTTCTTGTTGAGGAAGGCATTATCAATGAAGCGCAACTTGATCAAGC
ACTCGGCGCGCAAAGAAGTGGTGGACGCAAGCTTGGCGATACGCTGATTGATTTGGGTTTTTTGTCTGAACAACAGATGC
TGGCGTTTTTGTCTCAGCAACTTGATCTGCCTTTAATTGACTTAAATCGAGCCACGGTCGATATCGATGCAGTGCAGTTG
TTACCAGAAGTGCATGCTCGTCGTTTACGCGCATTGGTGATTGGCCGTAATGGCGACACGTTGCGCATTGCGATGAGTGA
TCCTGCGGACCTTTTTGCACAAGAAGCGTTGATCGGTCAGCTAGGACAATATGGCATTGAGTTTGTTATCGCGCCAGAAA
GGCAATTGGTGGAAGGCTTTGACCGCTATTATCGCCGTACCAAAGAGATTGCCAACTTTGCCGAGCAGTTGCAAGCGGAA
CACCAAGTCAACGATGCATTCGATTTCAATATTGAAGATGATGACAGTGATGAGGTAACGGTTGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATTCAGGTGGGGGCGTCGGATATCCATATCGAACCAGACGCCAATGTGTTGCGTTTGCGCCAAC
GTATTGATGGCGTGTTGCACGAAACCTTGCTCAATGAAGTCAACATCGCGCCAGCATTGGTACTGAGATTGAAACTCATG
GCCAACTTGGATATTTCAGAAAAGCGTCTGCCACAAGATGGCCGTTTTAACATCCGCTCCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAGTCGGTGGTAATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCCGGTTTGCCGGACGATCTTTTAGTGCGTCTGCGCCGACAGTTGAAACGTCCGCATGGCATGATCCTTGTT
ACTGGCCCTACGGGTTCTGGTAAAACCACCACGCTGTATGGTGCTCTAACAGAATTAAACCAACCGGGGAAAAAGATCAT
CACAGCGGAAGATCCGGTTGAATACCGACTGTCGCGTGTCAACCAAGTGCAGATCAATCCGAAGATCAACCTTGATTTTT
CCACGGTGTTAAGAACGTTCCTCCGTCAGGATCCCGATATCATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCCTGCGAGCGGCGCTGACCGGTCACTTAGTATTGAGTACCTTACACACCAATGACGCCGTAGACAGTGCGCTTCG
TATGATTGACATGGGGGCACCTGGTTATCTGGTCGCGAGTGCCGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGCAAGACAGAAGATCATGTTGGCGAGTCACGCAAAGCGTGGTTGGCACAACGCTTCCCAAATCAAGTG
GAAGCGGGTTTTGTGAAAGGGCGCGGTTGCCAAAACTGTAACTTAACAGGCTATCGCGGCCGTATTGGTGTGTTTGAAAT
GCTGGAACTTGAACATGGAATGATGGACGCGTTGCGCGCCAATGATGCCGTCGAGTTTGCGCATGCTGCGCGTCGTTCGC
CAGGCTATAAACCTCTGTTAGCGTCGGCAATGGAGTTGGCGCTACAAGGCGTAGTGAGCTTAGACGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.104

97.387

0.371
  pilB Vibrio cholerae strain A1552

37.699

98.432

0.371