Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   NX823_RS00020 Genome accession   NZ_CP103352
Coordinates   2362..3474 (+) Length   370 a.a.
NCBI ID   WP_014478578.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM117508     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..8474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX823_RS00010 (NX823_00010) dnaN 864..2000 (+) 1137 WP_003226811.1 DNA polymerase III subunit beta -
  NX823_RS00015 (NX823_00015) rlbA 2131..2346 (+) 216 WP_003226810.1 ribosome maturation protein RlbA -
  NX823_RS00020 (NX823_00020) recF 2362..3474 (+) 1113 WP_014478578.1 DNA replication/repair protein RecF Machinery gene
  NX823_RS00025 (NX823_00025) remB 3492..3737 (+) 246 WP_003219266.1 extracellular matrix regulator RemB -
  NX823_RS00030 (NX823_00030) gyrB 3792..5708 (+) 1917 WP_258996692.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  NX823_RS00035 (NX823_00035) gyrA 5919..8384 (+) 2466 WP_003244540.1 DNA topoisomerase (ATP-hydrolyzing) subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42304.34 Da        Isoelectric Point: 7.3660

>NTDB_id=722258 NX823_RS00020 WP_014478578.1 2362..3474(+) (recF) [Bacillus subtilis strain SRCM117508]
MYIQNLELSSYRNYDHAELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDKDYAKIEGRVMKQNGA
IPMQLVISKKGKKGKVNHIEQQKLSQYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLHDLSLYQKILSQR
NHFLKQLQTRKQTDRTMLDVLTDQLIEVAAKVVVKRLQFTAQLEKWAQPIHAGISRGLEELTLKYHTALDVSDPLDLSKI
GDSYQEAFSKLREKEIERGVTLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHETLRQAGMFRVQNGALVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=722258 NX823_RS00020 WP_014478578.1 2362..3474(+) (recF) [Bacillus subtilis strain SRCM117508]
TTGTATATCCAGAACTTAGAACTTTCATCTTACCGCAACTACGACCATGCTGAACTTCAATTTGAAAATAAAGTAAATGT
GATCATCGGAGAAAACGCCCAGGGGAAGACAAACCTCATGGAGGCGATCTATGTCTTGTCCATGGCGAAATCGCACCGGA
CATCAAATGACAAAGAACTTATACGGTGGGACAAAGACTATGCTAAAATAGAGGGAAGAGTGATGAAGCAAAACGGGGCG
ATCCCGATGCAGCTCGTCATCTCCAAAAAGGGTAAAAAGGGCAAGGTCAATCATATTGAACAGCAAAAGCTCAGCCAGTA
TGTCGGGGCCCTCAACACCATTATGTTCGCGCCGGAAGATTTAAATCTTGTAAAGGGAAGCCCTCAAGTGAGAAGGCGGT
TTCTTGACATGGAAATTGGACAGGTTTCTCCCGTCTATCTTCATGATCTTTCTCTTTACCAGAAAATCCTTTCCCAGCGG
AATCATTTTTTGAAACAGCTGCAAACAAGAAAACAAACTGACCGGACGATGCTCGATGTTCTGACCGATCAGCTTATAGA
AGTTGCAGCAAAAGTCGTCGTAAAACGCCTGCAGTTTACAGCACAGCTCGAGAAATGGGCGCAGCCCATCCATGCAGGCA
TCTCAAGAGGGCTTGAAGAACTTACCCTGAAATACCATACAGCTCTTGATGTATCAGATCCCCTAGATTTGTCGAAAATA
GGAGATAGCTATCAAGAAGCGTTTTCTAAATTAAGAGAAAAAGAAATTGAGCGTGGTGTGACGCTGTCAGGGCCTCATCG
CGATGATGTTCTTTTCTATGTGAACGGACGCGATGTGCAGACGTATGGTTCTCAAGGACAGCAGCGAACGACGGCGTTGT
CCCTTAAGCTGGCGGAGATTGACCTGATCCATGAAGAAATCGGAGAATATCCCATTTTACTATTGGATGATGTACTGAGT
GAACTGGATGATTATCGCCAGTCACACTTGCTTCATACGATCCAAGGCCGTGTACAAACGTTTGTCACAACGACAAGCGT
TGATGGCATTGATCACGAAACCTTACGTCAAGCAGGAATGTTCCGTGTGCAAAATGGTGCGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-350)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

99.459

100

0.995