Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NX819_RS13205 Genome accession   NZ_CP103351
Coordinates   2566698..2567108 (-) Length   136 a.a.
NCBI ID   WP_259266252.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM115947     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2561698..2572108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX819_RS13175 (NX819_13175) - 2561785..2561925 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  NX819_RS13180 (NX819_13180) yqeF 2562231..2562962 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  NX819_RS13185 (NX819_13185) cwlH 2563214..2563966 (-) 753 WP_046381197.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  NX819_RS13190 (NX819_13190) yqeD 2564153..2564779 (+) 627 WP_046381198.1 TVP38/TMEM64 family protein -
  NX819_RS13195 (NX819_13195) gnd 2564798..2565691 (-) 894 WP_038429331.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NX819_RS13200 (NX819_13200) yqeB 2565943..2566665 (+) 723 WP_010886572.1 hypothetical protein -
  NX819_RS13205 (NX819_13205) nucA/comI 2566698..2567108 (-) 411 WP_259266252.1 sporulation-specific Dnase NucB Machinery gene
  NX819_RS13210 (NX819_13210) sigK 2567304..2568032 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  NX819_RS13215 (NX819_13215) - 2568032..2568148 (+) 117 WP_122894874.1 hypothetical protein -
  NX819_RS13220 (NX819_13220) - 2568114..2568310 (-) 197 Protein_2556 recombinase family protein -
  NX819_RS13225 (NX819_13225) - 2568268..2568432 (-) 165 WP_080265384.1 hypothetical protein -
  NX819_RS13230 (NX819_13230) - 2568865..2569458 (-) 594 WP_029318098.1 TetR/AcrR family transcriptional regulator -
  NX819_RS13235 (NX819_13235) - 2569616..2570611 (+) 996 WP_029318099.1 acryloyl-CoA reductase -
  NX819_RS13240 (NX819_13240) - 2570939..2571172 (+) 234 Protein_2560 helix-turn-helix domain-containing protein -
  NX819_RS13245 (NX819_13245) arsC 2571383..2571802 (-) 420 WP_029318100.1 arsenate reductase (thioredoxin) -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 15011.00 Da        Isoelectric Point: 5.7414

>NTDB_id=722231 NX819_RS13205 WP_259266252.1 2566698..2567108(-) (nucA/comI) [Bacillus subtilis strain SRCM115947]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVRFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=722231 NX819_RS13205 WP_259266252.1 2566698..2567108(-) (nucA/comI) [Bacillus subtilis strain SRCM115947]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCGGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.739

84.559

0.522