Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NT399_RS02460 Genome accession   NZ_CP103338
Coordinates   516827..517627 (+) Length   266 a.a.
NCBI ID   WP_004738086.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain AB105     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 511827..522627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NT399_RS02435 (NT399_02435) - 512737..514035 (+) 1299 WP_001991191.1 glycosyltransferase family 1 protein -
  NT399_RS02440 (NT399_02440) - 514060..514611 (+) 552 WP_000669565.1 phosphatase PAP2 family protein -
  NT399_RS02445 (NT399_02445) iscX 514711..514911 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  NT399_RS02450 (NT399_02450) ndk 515025..515456 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  NT399_RS02455 (NT399_02455) rlmN 515579..516811 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  NT399_RS02460 (NT399_02460) pilF 516827..517627 (+) 801 WP_004738086.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NT399_RS02465 (NT399_02465) - 517618..518409 (+) 792 WP_000405161.1 RodZ domain-containing protein -
  NT399_RS02470 (NT399_02470) ispG 518431..519546 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  NT399_RS02475 (NT399_02475) hisS 519543..520835 (+) 1293 WP_149958483.1 histidine--tRNA ligase -
  NT399_RS02480 (NT399_02480) - 520864..521562 (+) 699 WP_000058788.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30094.44 Da        Isoelectric Point: 9.2307

>NTDB_id=722058 NT399_RS02460 WP_004738086.1 516827..517627(+) (pilF) [Acinetobacter baumannii strain AB105]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=722058 NT399_RS02460 WP_004738086.1 516827..517627(+) (pilF) [Acinetobacter baumannii strain AB105]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGTGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

100

100

1
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726