Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   NX062_RS12625 Genome accession   NZ_CP103328
Coordinates   2359142..2359909 (-) Length   255 a.a.
NCBI ID   WP_014477369.1    Uniprot ID   A0A0D1L7G7
Organism   Bacillus subtilis strain KC14-1     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2354142..2364909
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX062_RS12605 (NX062_12600) ppsR 2354317..2355129 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  NX062_RS12610 (NX062_12605) ccpN 2355155..2355793 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  NX062_RS12615 (NX062_12610) glyS 2355926..2357965 (-) 2040 WP_129110529.1 glycine--tRNA ligase subunit beta -
  NX062_RS12620 (NX062_12615) glyQ 2357958..2358845 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  NX062_RS12625 (NX062_12620) recO 2359142..2359909 (-) 768 WP_014477369.1 DNA repair protein RecO Machinery gene
  NX062_RS12630 (NX062_12625) yqzL 2359946..2360089 (-) 144 WP_003230053.1 YqzL family protein -
  NX062_RS12635 (NX062_12630) era 2360237..2361142 (-) 906 WP_087614624.1 GTPase Era -
  NX062_RS12640 (NX062_12635) - 2361123..2361533 (-) 411 WP_032722130.1 cytidine deaminase -
  NX062_RS12645 (NX062_12640) unpK 2361652..2362023 (-) 372 WP_015384119.1 diacylglycerol kinase family protein -
  NX062_RS12650 (NX062_12645) ybeY 2362004..2362477 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  NX062_RS12655 (NX062_12650) pgpH 2362478..2364613 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=721991 NX062_RS12625 WP_014477369.1 2359142..2359909(-) (recO) [Bacillus subtilis strain KC14-1]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=721991 NX062_RS12625 WP_014477369.1 2359142..2359909(-) (recO) [Bacillus subtilis strain KC14-1]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAATTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1L7G7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.608

100

0.996