Detailed information NTG0721772

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: C0965_RS01720
• Genome accession: NZ_CP103293
• Coordinates: 353093..354466 (+)
• Length: 457 a.a.
• NCBI ID: WP_004562734.1
• Uniprot ID: A0A806TIP1
• Organism: Limosilactobacillus fermentum strain LfU21
• Function: homologous recombination (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Prophage	346125..355632	353093..354466	within	0

Gene organization within MGE regions

Location: 346125..355632

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C0965_RS01680 (C0965_001680)	-	346125..347000 (+)	876	WP_004562728.1	alpha/beta hydrolase	-
C0965_RS01685 (C0965_001685)	-	347317..348654 (-)	1338	WP_035437082.1	aminopeptidase C	-
C0965_RS01690 (C0965_001690)	-	348718..349332 (-)	615	WP_024500694.1	sugar O-acetyltransferase	-
C0965_RS01695 (C0965_001695)	rpiA	349360..350046 (-)	687	WP_051132373.1	ribose-5-phosphate isomerase RpiA	-
C0965_RS01700 (C0965_001700)	tnpA	350079..350531 (-)	453	Protein_319	IS200/IS605 family transposase	-
C0965_RS01705 (C0965_001705)	-	350610..351860 (+)	1251	WP_021349622.1	RNA-guided endonuclease TnpB family protein	-
C0965_RS01710 (C0965_001710)	-	352015..352341 (-)	327	WP_035437471.1	GNAT family N-acetyltransferase	-
C0965_RS01715 (C0965_001715)	-	352541..353080 (+)	540	WP_004563338.1	dUTP diphosphatase	-
C0965_RS01720 (C0965_001720)	radA	353093..354466 (+)	1374	WP_004562734.1	DNA repair protein RadA	Machinery gene
C0965_RS01725 (C0965_001725)	-	354499..355632 (+)	1134	WP_004562735.1	PIN/TRAM domain-containing protein	-

Sequence

Protein

• Length: 457 a.a.
• Molecular weight: 49381.79 Da
• Isoelectric Point: 8.7358

>NTDB_id=721772 C0965_RS01720 WP_004562734.1 353093..354466(+) (radA) [Limosilactobacillus fermentum strain LfU21]
MAKAKTHFVCQNCGYNSPRYLGRCPNCGQWSTLVEEVEQASTPAAKNATATLTGIVARPQRIQEIDTKKTPRVKTRLNEL
NRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSVEHHKVLYVSGEESASQIKLRAQRLDVSGEDFYIYPETNMEAIR
ATIDSIQPEFVIIDSVQTMQAADVTSAIGSVSQIRAVTAQLMQIAKGQNITVFVVGHVTKGGVLAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMNTRGLTEVANPSEIFLEERLKDATGSAVVVSLEGTRPILVEIQALITPTVF
GNAQRTATGLNRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVAIASSYRDKGTRPTDAFVGEVGLT
GEIRRVSRIEQRVAEAAKLGFKRILVPKNNLAGWNPPQDIEVVGVTTLGEALKIALG

Nucleotide

• Length: 1374 bp

>NTDB_id=721772 C0965_RS01720 WP_004562734.1 353093..354466(+) (radA) [Limosilactobacillus fermentum strain LfU21]
ATGGCAAAAGCCAAAACACACTTTGTCTGCCAAAACTGCGGTTACAATTCACCGCGCTATTTAGGGCGGTGTCCCAATTG
TGGCCAGTGGAGTACCTTAGTTGAAGAAGTCGAGCAGGCAAGTACCCCGGCGGCTAAAAACGCTACGGCAACCTTAACCG
GGATTGTGGCCCGGCCCCAGCGAATCCAAGAAATTGACACCAAAAAAACGCCCCGGGTTAAGACCCGCTTAAATGAGTTA
AACCGGGTGCTAGGTGGCGGAATTGTACCCGGCTCGTTGGTCTTAATTGGTGGGGATCCCGGGATCGGAAAGTCCACCCT
GCTCTTACAAGTGTCGGGGCAACTGAGCGTGGAACACCACAAGGTTCTTTACGTTTCCGGGGAAGAATCGGCCTCCCAAA
TTAAATTGCGGGCCCAACGCTTAGACGTTTCCGGCGAGGACTTTTATATCTACCCGGAAACCAACATGGAAGCCATCCGG
GCGACGATTGATTCCATTCAGCCCGAGTTTGTAATCATTGACTCGGTTCAGACGATGCAGGCGGCCGACGTGACCTCGGC
GATTGGGTCGGTCTCCCAAATCCGGGCGGTCACCGCCCAGTTGATGCAGATTGCCAAGGGGCAAAACATCACGGTCTTCG
TGGTTGGTCACGTCACCAAGGGTGGGGTCTTAGCCGGGCCCAAGATCTTGGAACACATGGTTGACACGGTGCTGTACTTT
GAAGGGGACCTCCACCACACCTACCGGATTTTACGGTCGGTCAAAAACCGGTTTGGGTCCACCAATGAGTTAGGGATCTT
CGAAATGAATACCCGCGGTTTGACCGAGGTCGCAAACCCCTCGGAAATCTTTTTGGAAGAGCGGCTTAAGGATGCCACCG
GGTCGGCCGTGGTCGTATCCCTGGAGGGAACCCGACCGATCCTGGTTGAAATCCAGGCCTTGATCACGCCAACCGTCTTT
GGAAACGCCCAAAGAACGGCGACAGGTTTAAACCGCAACCGGGTTTCCTTGATCATGGCGGTCCTAGAAAAACGGGCGAA
CCTGCTCTTACAAAACCAGGATGCCTACTTGAAGGCGGCCGGGGGCGTTAAGTTAGACGAGCCGGCCATCGACCTTGCGA
TCGCGGTGGCAATTGCTTCTTCTTACCGGGATAAGGGGACCCGGCCGACCGACGCCTTCGTCGGTGAAGTTGGCTTAACC
GGGGAGATCCGTCGGGTGAGCCGAATTGAACAGCGGGTGGCCGAAGCCGCTAAACTAGGCTTTAAGCGGATTTTAGTGCC
CAAAAACAATTTAGCGGGTTGGAACCCGCCGCAAGACATCGAAGTCGTGGGGGTAACGACCCTTGGCGAAGCACTTAAAA
TTGCCCTTGGATAG

Domains

Predicted by InterproScan.

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

• Subunit ChlI of Mg-chelatase (ChlI) (364-430)

• AAA domain (AAA_25) (64-222)

3D structure

• AlphaFold DB: A0A806TIP1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Streptococcus mitis SK321	68.212	99.125	0.676
radA	Streptococcus mitis NCTC 12261	67.991	99.125	0.674
radA	Streptococcus pneumoniae Rx1	67.991	99.125	0.674
radA	Streptococcus pneumoniae D39	67.991	99.125	0.674
radA	Streptococcus pneumoniae R6	67.991	99.125	0.674
radA	Streptococcus pneumoniae TIGR4	67.991	99.125	0.674
radA	Bacillus subtilis subsp. subtilis str. 168	63.676	100	0.637