Detailed information NTG0072168

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: EL334_RS10545
• Genome accession: NZ_AP019192
• Coordinates: 1935181..1935642 (-)
• Length: 153 a.a.
• NCBI ID: WP_033705217.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain ASP0581
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1930181..1940642

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL334_RS10515 (ASP0581_19510)	-	1930492..1931316 (-)	825	WP_000727904.1	membrane protein insertase YidC	-
EL334_RS10520 (ASP0581_19520)	rnpA	1931291..1931662 (-)	372	WP_000739246.1	ribonuclease P protein component	-
EL334_RS12550	-	1931679..1931810 (-)	132	WP_000768904.1	hypothetical protein	-
EL334_RS10525 (ASP0581_19530)	-	1931811..1933001 (-)	1191	WP_000167766.1	acetate kinase	-
EL334_RS10530 (ASP0581_19540)	comYH	1933052..1934005 (-)	954	WP_000345119.1	class I SAM-dependent methyltransferase	Machinery gene
EL334_RS10535 (ASP0581_19550)	-	1934066..1934653 (-)	588	WP_000679774.1	class I SAM-dependent methyltransferase	-
EL334_RS10540 (ASP0581_19560)	comGG/cglG	1934790..1935203 (-)	414	WP_000265630.1	competence type IV pilus minor pilin ComGG	Machinery gene
EL334_RS10545	comGF/cglF	1935181..1935642 (-)	462	WP_033705217.1	competence type IV pilus minor pilin ComGF	Machinery gene
EL334_RS10550 (ASP0581_19580)	comGE/cglE	1935605..1935907 (-)	303	WP_000413380.1	competence type IV pilus minor pilin ComGE	Machinery gene
EL334_RS10555 (ASP0581_19590)	comGD/cglD	1935870..1936274 (-)	405	WP_000588005.1	competence type IV pilus minor pilin ComGD	Machinery gene
EL334_RS10560 (ASP0581_19600)	comGC/cglC	1936267..1936593 (-)	327	WP_000738638.1	comG operon protein ComGC	Machinery gene
EL334_RS10565 (ASP0581_19610)	comGB/cglB	1936595..1937611 (-)	1017	WP_013193332.1	competence type IV pilus assembly protein ComGB	Machinery gene
EL334_RS10570 (ASP0581_19620)	comGA/cglA/cilD	1937559..1938500 (-)	942	WP_126432103.1	competence type IV pilus ATPase ComGA	Machinery gene
EL334_RS10575 (ASP0581_19630)	-	1938576..1938941 (-)	366	WP_000286415.1	DUF1033 family protein	-
EL334_RS10580 (ASP0581_19640)	-	1939092..1940150 (-)	1059	WP_000649468.1	zinc-dependent alcohol dehydrogenase family protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17889.35 Da
• Isoelectric Point: 6.4832

>NTDB_id=72168 EL334_RS10545 WP_033705217.1 1935181..1935642(-) (comGF/cglF) [Streptococcus pneumoniae strain ASP0581]
MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLISEVRYQQQSEQKEWFLFVDQLEAELDRSQFEKVEGN
RFYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide

• Length: 462 bp

>NTDB_id=72168 EL334_RS10545 WP_033705217.1 1935181..1935642(-) (comGF/cglF) [Streptococcus pneumoniae strain ASP0581]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTGCT
CGTCATCAGTGGAGGCTTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGTTCTTGTTTGTGGATCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTGGAAGGCAAT
CGTTTCTATATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	92.81	100	0.928
comGF/cglF	Streptococcus pneumoniae D39	92.81	100	0.928
comGF/cglF	Streptococcus pneumoniae R6	92.81	100	0.928
comGF/cglF	Streptococcus pneumoniae TIGR4	92.81	100	0.928
comGF/cglF	Streptococcus mitis SK321	92.157	100	0.922
comGF/cglF	Streptococcus mitis NCTC 12261	90.85	100	0.909