Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   EL334_RS10055 Genome accession   NZ_AP019192
Coordinates   1855954..1856778 (-) Length   274 a.a.
NCBI ID   WP_001036786.1    Uniprot ID   A0A0B7LDF1
Organism   Streptococcus pneumoniae strain ASP0581     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1850954..1861778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL334_RS10050 (ASP0581_18760) - 1854332..1855663 (-) 1332 WP_000390042.1 hemolysin family protein -
  EL334_RS10055 (ASP0581_18770) endA 1855954..1856778 (-) 825 WP_001036786.1 DNA-entry nuclease EndA Machinery gene
  EL334_RS10060 (ASP0581_18780) - 1856817..1857005 (-) 189 WP_001813390.1 DNA-directed RNA polymerase subunit beta -
  EL334_RS10065 (ASP0581_18790) murA 1856998..1858281 (-) 1284 WP_000358028.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  EL334_RS10070 (ASP0581_18800) - 1858305..1858535 (-) 231 WP_000250384.1 DUF1146 family protein -
  EL334_RS10075 (ASP0581_18810) sepM 1858593..1859630 (-) 1038 WP_000727277.1 SepM family pheromone-processing serine protease Regulator
  EL334_RS10080 (ASP0581_18820) coaD 1859614..1860102 (-) 489 WP_001280746.1 pantetheine-phosphate adenylyltransferase -
  EL334_RS10085 (ASP0581_18830) rsmD 1860092..1860631 (-) 540 WP_000706957.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  EL334_RS10090 (ASP0581_18840) asnA 1860696..1861688 (-) 993 WP_033705591.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29921.59 Da        Isoelectric Point: 10.1197

>NTDB_id=72161 EL334_RS10055 WP_001036786.1 1855954..1856778(-) (endA) [Streptococcus pneumoniae strain ASP0581]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=72161 EL334_RS10055 WP_001036786.1 1855954..1856778(-) (endA) [Streptococcus pneumoniae strain ASP0581]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGGCACCTAATAGTCCCAAAACCAATCTTAGTCAGAAAAAACAAGCGTCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCTCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7LDF1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

99.635

100

0.996
  endA Streptococcus pneumoniae D39

99.635

100

0.996
  endA Streptococcus pneumoniae R6

99.635

100

0.996
  endA Streptococcus pneumoniae TIGR4

99.635

100

0.996

Multiple sequence alignment