Detailed information    

insolico Bioinformatically predicted

Overview

Name   recS   Type   Machinery gene
Locus tag   PUW21_RS12315 Genome accession   NZ_CP118167
Coordinates   2375832..2377322 (-) Length   496 a.a.
NCBI ID   WP_003230528.1    Uniprot ID   P50729
Organism   Bacillus subtilis strain 21855     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2370832..2382322
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW21_RS12285 (PUW21_12285) gudB 2370986..2372260 (-) 1275 WP_003230536.1 NAD-specific glutamate dehydrogenase -
  PUW21_RS12290 (PUW21_12290) mecB 2372416..2373000 (-) 585 WP_003230533.1 genetic competence negative regulator -
  PUW21_RS12295 (PUW21_12295) ypbG 2373159..2373938 (-) 780 WP_003230532.1 metallophosphoesterase -
  PUW21_RS12300 (PUW21_12300) ypbF 2374024..2374467 (-) 444 WP_003230531.1 YpbF family protein -
  PUW21_RS12305 (PUW21_12305) ypbE 2374530..2375252 (-) 723 WP_004398603.1 LysM peptidoglycan-binding domain-containing protein -
  PUW21_RS12310 (PUW21_12310) ypbD 2375203..2375805 (-) 603 WP_003230529.1 CPBP family intramembrane glutamic endopeptidase -
  PUW21_RS12315 (PUW21_12315) recS 2375832..2377322 (-) 1491 WP_003230528.1 ATP-dependent DNA helicase RecQ Machinery gene
  PUW21_RS12320 (PUW21_12320) gmmB 2377315..2378373 (-) 1059 WP_004398594.1 helix-turn-helix domain-containing protein -
  PUW21_RS12325 (PUW21_12325) fer 2378639..2378887 (+) 249 WP_003225461.1 ferredoxin -
  PUW21_RS12330 (PUW21_12330) fmnP 2378927..2379499 (-) 573 WP_004399159.1 riboflavin transporter FmnP -
  PUW21_RS12335 (PUW21_12335) serA 2379996..2381573 (+) 1578 WP_004398713.1 phosphoglycerate dehydrogenase -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 56508.11 Da        Isoelectric Point: 6.6626

>NTDB_id=721527 PUW21_RS12315 WP_003230528.1 2375832..2377322(-) (recS) [Bacillus subtilis strain 21855]
MTKLQQTLYQFFGFTSFKKGQQDIIESILSGKDTIAMLPTGGGKSLCYQLPGYMLDGMVLIVSPLLSLMEDQVQQLKARG
EKRAAALNSMLNRQERQFVLEHIHRYKFLYLSPEALQSPYVLEKLKSVPISLFVIDEAHCISEWGHDFRPDYSKLGQLRK
KLGHPPVLALTATATKETLQDVMNLLELQHAVRHLNSVNRPNIALRVENAADTAEKIDRVIQLVENLQGPGIVYCPTRKW
AKELAGEIKSKTSSRADFYHGGLESGDRILIQQQFIHNQLDVICCTNAFGMGVDKPDIRYVIHFHLPQTAEAFMQEIGRA
GRDGKPSVSILLRAPGDFELQEQIIQMESVTAEEIADVIRVLEKTEERDERRLRDVLLQYGVGETQARMMIHLFMQGKTS
VELMKKEISYRMELKLEKMHRVSFLLQRDGCLRQALLTYFDESYEPDDGNLPCCSHCGFDLSLYEQKGERSKMAPLDSWS
SELHRIFSLQTVGELN

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=721527 PUW21_RS12315 WP_003230528.1 2375832..2377322(-) (recS) [Bacillus subtilis strain 21855]
ATGACTAAATTACAGCAAACGTTATATCAGTTTTTTGGTTTTACTTCCTTTAAAAAAGGGCAGCAGGACATTATTGAAAG
CATACTCAGCGGGAAGGATACCATTGCAATGCTCCCGACTGGGGGAGGGAAATCGCTTTGCTACCAGCTGCCTGGTTATA
TGCTTGATGGCATGGTATTAATCGTTTCTCCACTGCTTTCTTTAATGGAGGATCAGGTGCAGCAGCTCAAAGCCCGGGGG
GAAAAGCGTGCTGCAGCTTTGAACAGCATGCTTAACAGGCAGGAAAGACAATTTGTTTTAGAACATATTCATCGATATAA
ATTTTTGTACTTGTCTCCTGAAGCTTTACAGTCTCCATATGTATTGGAAAAATTGAAAAGCGTTCCGATTAGCCTATTTG
TTATTGACGAAGCGCATTGTATTTCTGAATGGGGACACGACTTCAGGCCTGATTATTCAAAGCTCGGACAGCTGAGAAAA
AAACTTGGACACCCACCCGTTCTGGCATTGACCGCCACGGCTACAAAGGAAACGCTGCAAGATGTCATGAACCTGCTGGA
GCTGCAGCATGCTGTACGCCATCTCAACTCAGTCAACCGCCCAAACATCGCACTGAGGGTTGAAAATGCGGCGGATACTG
CCGAAAAAATAGATAGGGTCATTCAGCTGGTTGAAAACCTGCAAGGTCCGGGAATTGTTTATTGTCCAACACGGAAATGG
GCTAAGGAATTAGCGGGTGAAATTAAAAGCAAAACGAGCAGCAGAGCCGACTTTTACCATGGCGGTTTGGAGTCAGGGGA
CAGGATTTTAATTCAGCAGCAGTTTATTCATAACCAGCTTGATGTGATATGTTGTACGAATGCGTTTGGAATGGGTGTCG
ATAAACCTGATATCAGATATGTGATTCATTTTCATCTCCCGCAGACGGCAGAAGCTTTTATGCAGGAAATCGGCAGAGCG
GGACGCGACGGAAAGCCGAGTGTCAGCATTTTGCTGCGGGCGCCAGGGGATTTTGAATTGCAGGAACAAATTATCCAGAT
GGAGAGTGTCACAGCTGAAGAAATCGCCGACGTAATCCGTGTGCTAGAGAAGACGGAGGAGCGGGATGAGAGAAGGCTGC
GTGACGTATTACTTCAGTATGGCGTCGGAGAGACACAGGCCCGTATGATGATTCATCTCTTTATGCAGGGAAAAACATCA
GTTGAACTGATGAAGAAAGAAATTTCATATCGCATGGAGCTGAAGCTGGAAAAAATGCACCGTGTATCATTTTTGCTTCA
GCGTGACGGCTGTTTAAGGCAGGCACTGTTAACCTACTTTGATGAATCATACGAGCCTGATGACGGAAACCTCCCGTGCT
GTTCACATTGCGGTTTTGATTTATCCTTGTATGAACAAAAAGGGGAACGGAGTAAAATGGCGCCATTAGACAGCTGGAGT
TCGGAATTGCACCGGATATTCAGCTTGCAGACTGTAGGTGAGCTGAACTGA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(20-180)

Helicase conserved C-terminal domain (Helicase_C)

(215-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P50729

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recS Bacillus subtilis subsp. subtilis str. 168

100

100

1