Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   PUW21_RS10370 Genome accession   NZ_CP118167
Coordinates   2073496..2075271 (-) Length   591 a.a.
NCBI ID   WP_004399229.1    Uniprot ID   O34748
Organism   Bacillus subtilis strain 21855     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2068496..2080271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW21_RS10350 (PUW21_10350) desE 2068882..2069940 (+) 1059 WP_004399588.1 fatty acid desaturase DesE -
  PUW21_RS10355 (PUW21_10355) desK 2070060..2071172 (+) 1113 WP_003231271.1 two-component sensor histidine kinase DesK -
  PUW21_RS10360 (PUW21_10360) desR 2071191..2071790 (+) 600 WP_004399356.1 two-component system response regulator DesR -
  PUW21_RS10365 (PUW21_10365) walL 2072385..2073248 (-) 864 WP_003231267.1 3D domain-containing protein -
  PUW21_RS10370 (PUW21_10370) recQ 2073496..2075271 (-) 1776 WP_004399229.1 DNA helicase RecQ Machinery gene
  PUW21_RS10380 (PUW21_10380) azoJ 2075836..2076462 (-) 627 WP_003231260.1 FMN-dependent NADH-azoreductase AzoJ -
  PUW21_RS10385 (PUW21_10385) yocK 2076613..2077104 (-) 492 WP_003231259.1 TraR/DksA family transcriptional regulator -
  PUW21_RS10390 (PUW21_10390) - 2077179..2077511 (-) 333 WP_003231257.1 hypothetical protein -
  PUW21_RS10395 (PUW21_10395) - 2077589..2077816 (+) 228 WP_004399329.1 hypothetical protein -
  PUW21_RS10400 (PUW21_10400) yocM 2077803..2078279 (-) 477 WP_003231255.1 salt stress-responsive protein YocM -
  PUW21_RS10405 (PUW21_10405) - 2078346..2078609 (+) 264 WP_004399338.1 hypothetical protein -
  PUW21_RS10410 (PUW21_10410) - 2078614..2078847 (+) 234 WP_003231251.1 hypothetical protein -
  PUW21_RS10415 (PUW21_10415) yozO 2078933..2079277 (-) 345 WP_003220141.1 PH domain-containing protein -
  PUW21_RS10420 (PUW21_10420) yozC 2079634..2079837 (-) 204 WP_003231245.1 DUF6501 family protein -

Sequence

Protein


Download         Length: 591 a.a.        Molecular weight: 67352.87 Da        Isoelectric Point: 6.2902

>NTDB_id=721522 PUW21_RS10370 WP_004399229.1 2073496..2075271(-) (recQ) [Bacillus subtilis strain 21855]
MLHRAQSLLAHYFGYEKFRSGQDEAIRLVTEARQNTACIMPTGGGKSICYQIPALMFEGTTIVISPLISLMKDQVDALEE
AGINAAYINSTQSNQEIYERLNGLKEGAYKLFYITPERLTSIEFIRILQGIDVPLVAIDEAHCISQWGHDFRPSYRNIEI
LFRELHDKPVIMALTATATPEVHDDICKQLHIQKENTVYTGFSRENLTFKVVKGENKDRFIDEYVQNNRHEAGIVYTATR
KEADRIYERLKRNQVRAGRYHGGLADDVRKEQQERFLNDELQVMVATSAFGMGIDKSNIRFVLHAQIPKDMESYYQEAGR
AGRDGLASECVLLFSPQDIMVQRFLIEQSEHEEKQKQDLKKLRQMVDYCHTEDCLQRFILMYFGEKEPDACGQCGNCTDT
RAAHDVTREAQMVLSCIIRMKERFGKTMVAQVLAGSKNKKVLENGFSDLSTYGILKHQSVGEISDFIEFLISDDFIRMSD
GTFPTLFVSSKGRNVLKGELSVARKEALKAAAITENDELFERLRMVRKEIAAEQGVPPFVVFSDQTLKEMSGKQPVNDDE
LLSIKGVGEQKRAKYGRLFLQEIQAYARMTD

Nucleotide


Download         Length: 1776 bp        

>NTDB_id=721522 PUW21_RS10370 WP_004399229.1 2073496..2075271(-) (recQ) [Bacillus subtilis strain 21855]
ATGTTACATAGAGCCCAATCCCTTCTGGCTCATTATTTTGGTTATGAGAAGTTTCGGAGCGGCCAAGATGAAGCGATCCG
CTTGGTGACGGAAGCGAGGCAAAATACGGCCTGCATTATGCCGACGGGAGGCGGCAAATCGATTTGCTATCAGATTCCGG
CATTAATGTTTGAAGGGACGACGATCGTCATTTCACCGTTGATTTCCTTGATGAAGGATCAAGTTGATGCGTTAGAAGAA
GCGGGGATTAACGCCGCCTACATTAACAGCACACAATCCAATCAGGAAATATATGAGAGGCTGAACGGACTCAAAGAAGG
GGCTTATAAGCTTTTTTACATTACACCAGAGCGACTAACGTCCATAGAGTTTATCCGGATTTTGCAGGGCATAGATGTTC
CTTTGGTCGCAATAGACGAGGCGCATTGTATTTCTCAATGGGGCCATGATTTCAGGCCAAGCTACAGGAATATTGAAATC
TTGTTCCGTGAATTACACGACAAGCCTGTCATCATGGCGTTAACAGCAACAGCCACACCCGAGGTTCATGATGATATTTG
CAAACAGCTGCATATTCAAAAAGAAAACACGGTATATACCGGATTTTCCCGAGAGAATTTAACCTTTAAAGTAGTAAAAG
GAGAAAATAAAGATCGGTTTATAGACGAATATGTGCAAAACAACAGGCATGAAGCAGGAATTGTCTATACAGCGACCAGA
AAAGAAGCTGATCGGATATATGAGAGATTGAAACGAAATCAGGTCAGGGCAGGCCGGTATCATGGAGGTTTGGCTGATGA
TGTGAGAAAAGAGCAGCAGGAAAGATTTCTTAATGATGAGCTTCAAGTGATGGTGGCAACCTCTGCTTTTGGAATGGGTA
TTGATAAATCAAATATCAGATTTGTTCTGCATGCACAAATCCCGAAAGATATGGAAAGCTATTATCAAGAGGCAGGACGA
GCTGGACGGGACGGTCTTGCCAGTGAATGTGTTCTCCTGTTTTCACCGCAGGATATTATGGTGCAGCGCTTCTTAATTGA
GCAATCAGAACATGAAGAGAAACAAAAGCAGGATTTAAAAAAGCTGAGACAAATGGTCGACTACTGTCATACGGAAGATT
GTCTGCAGCGTTTTATCCTTATGTATTTCGGAGAAAAGGAGCCTGATGCGTGCGGACAGTGCGGAAACTGTACCGATACA
AGAGCGGCCCATGACGTGACGCGGGAGGCGCAAATGGTCTTATCTTGTATCATTCGCATGAAAGAACGGTTTGGCAAGAC
GATGGTTGCCCAGGTGCTTGCCGGCTCCAAAAATAAAAAAGTTCTTGAAAACGGCTTTTCCGACCTTTCAACATACGGGA
TATTAAAACACCAATCCGTTGGCGAAATCAGTGATTTTATCGAATTTCTTATTTCTGACGACTTCATCAGGATGTCAGAC
GGAACGTTTCCGACTCTTTTTGTCAGCAGTAAAGGAAGGAATGTGCTGAAAGGAGAATTATCAGTAGCGAGAAAAGAAGC
GTTAAAAGCGGCAGCGATTACTGAAAATGACGAGCTATTTGAACGATTGCGCATGGTTCGGAAAGAAATTGCGGCTGAGC
AGGGTGTTCCGCCATTTGTTGTTTTCTCTGACCAAACGTTAAAAGAAATGTCAGGAAAACAGCCCGTAAATGATGATGAA
CTTTTGTCTATAAAAGGAGTCGGAGAACAAAAAAGAGCAAAATACGGCCGGCTTTTTTTACAAGAAATACAGGCTTATGC
AAGGATGACAGACTAA

Domains

Predicted by InterProScan.

RQC domain (RQC)

(403-507)

RecQ zinc-binding (RecQ_Zn_bind)

(336-398)

DEAD/DEAH box helicase (DEAD)

(21-182)

Helicase conserved C-terminal domain (Helicase_C)

(218-324)

HRDC domain (HRDC)

(518-583)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O34748

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

100

100

1