Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   PUW21_RS06235 Genome accession   NZ_CP118167
Coordinates   1206857..1208686 (+) Length   609 a.a.
NCBI ID   WP_173401378.1    Uniprot ID   -
Organism   Bacillus subtilis strain 21855     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1201857..1213686
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW21_RS06200 (PUW21_06200) yjbC 1202529..1203107 (+) 579 WP_003224597.1 GNAT family N-acetyltransferase -
  PUW21_RS06205 (PUW21_06205) spx 1203288..1203683 (+) 396 WP_014476435.1 transcriptional regulator Spx -
  PUW21_RS06210 (PUW21_06210) yjbE 1203726..1204382 (-) 657 WP_003232944.1 TerC family protein -
  PUW21_RS06215 (PUW21_06215) - 1204552..1204692 (+) 141 WP_119122854.1 hypothetical protein -
  PUW21_RS06220 (PUW21_06220) mecA 1204659..1205315 (+) 657 WP_003245194.1 adaptor protein MecA Regulator
  PUW21_RS06225 (PUW21_06225) - 1205310..1205432 (-) 123 WP_003245684.1 hypothetical protein -
  PUW21_RS06230 (PUW21_06230) coiA 1205476..1206627 (+) 1152 WP_046380934.1 competence protein CoiA Machinery gene
  PUW21_RS06235 (PUW21_06235) pepF 1206857..1208686 (+) 1830 WP_173401378.1 oligoendopeptidase F Regulator
  PUW21_RS06240 (PUW21_06240) - 1208724..1208891 (-) 168 WP_003244944.1 hypothetical protein -
  PUW21_RS06245 (PUW21_06245) spxH 1209205..1210104 (-) 900 WP_024573348.1 protease adaptor protein SpxH -
  PUW21_RS06250 (PUW21_06250) yjbI 1210101..1210499 (-) 399 WP_046380935.1 thiol management oxidoreductase -
  PUW21_RS06255 (PUW21_06255) cwlQ 1210754..1211488 (-) 735 WP_046380936.1 bifunctional muramidase/murein lytic transglycosylase -
  PUW21_RS06260 (PUW21_06260) yjbK 1211503..1212075 (-) 573 WP_014479452.1 CYTH domain-containing protein -
  PUW21_RS06265 (PUW21_06265) - 1212200..1212568 (+) 369 WP_046380937.1 hypothetical protein -
  PUW21_RS06270 (PUW21_06270) yjbM 1212597..1213232 (+) 636 WP_003245294.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 70100.02 Da        Isoelectric Point: 5.2218

>NTDB_id=721493 PUW21_RS06235 WP_173401378.1 1206857..1208686(+) (pepF) [Bacillus subtilis strain 21855]
MAEEKKANQLPDRSEVKAEDTWRLEDIFPSDEAWNKEFQAVKELIPNLSKYKGKLADSADHLYEALTYQDKVMERLGRLY
TYAHMRSDQDTGNSFYQGLNDKAGNLYTQAASATAYLVPEILSIEEDKLQQFILEKEELKLYSHAIEEITKERPHVLSEK
EEALLAEASEVLGSSSNTFSVLNNADITFPSIKDEDGNEKQITHGNFINFLESENREVRKNAFDAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLVKTINKHLPLLHRYIALRKKVLELDEVHIYDLYTPLVKDAGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRNGAYSSGAYGTNPYILMNWHNNVNNLFTLVHEFGHS
VHSYYTRKHQPYPYGNYSIFVAEVASTTNEALLGEYLLNNLEDEKQRLYILNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLTKVYYDLNKKYFGDGMVIDKEIGLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVDRYIDFL
KAGSSQYPIDVLKKAGVDMTSPEPIEAACKMFEEKLDEMEELLMKVKQS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=721493 PUW21_RS06235 WP_173401378.1 1206857..1208686(+) (pepF) [Bacillus subtilis strain 21855]
ATGGCTGAGGAAAAAAAAGCAAACCAACTGCCTGACAGAAGTGAGGTAAAGGCAGAAGACACATGGAGACTTGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCTGTAAAAGAATTAATTCCGAATTTATCTAAGTATAAAGGAA
AGCTGGCAGATTCAGCTGATCATTTATACGAGGCTCTTACGTATCAAGATAAAGTGATGGAGCGGCTGGGTAGGCTGTAC
ACATATGCGCATATGCGCTCTGACCAGGATACTGGGAACTCCTTTTACCAGGGGCTGAATGACAAGGCGGGAAACCTGTA
TACACAGGCCGCAAGCGCGACAGCTTATCTGGTTCCGGAGATTTTATCCATAGAAGAAGACAAACTGCAGCAGTTCATTC
TTGAAAAAGAAGAATTAAAGCTGTACTCTCATGCGATTGAGGAGATTACAAAGGAACGTCCGCATGTGCTGAGCGAGAAG
GAAGAGGCACTGCTTGCTGAAGCCTCCGAGGTACTGGGGTCATCTTCAAATACATTCAGTGTGTTAAATAACGCAGATAT
AACGTTTCCATCCATTAAAGACGAAGATGGGAATGAAAAACAGATCACTCACGGCAACTTTATTAATTTCTTGGAAAGTG
AAAACCGTGAAGTCCGCAAAAATGCGTTTGACGCAGTGTATAAAACGTACGGACAATATAAAAATACAATGGCCACGACG
CTTAGCGGCACTGTGAAAAAGGACAACTTCTACGCGAGAGTGAAAAAGTACAAGTCCGCGCGTGAGGCTGCGCTTTCTAA
CAACAGTATTCCTGAGGAAGTATACGATAACCTTGTTAAGACGATTAATAAGCATTTGCCGCTCCTGCACCGCTATATTG
CGCTAAGAAAGAAAGTGCTTGAGCTTGATGAAGTGCATATCTATGACCTGTATACACCGCTTGTGAAAGATGCTGGGATG
AAGGTGACATACGAGGAAGCCAAAGATTACATGCTGAAAGGCCTTGCACCTTTAGGGGAAGAATATGCCTCTATCCTAAA
AGAAGGACTGGAAAACCGCTGGGTGGACGTTTACGAAAATAAAGGCAAACGCAATGGGGCTTATTCATCAGGAGCTTACG
GCACGAATCCGTATATTTTGATGAACTGGCATAATAACGTCAATAATCTCTTTACGCTCGTGCACGAGTTTGGACATTCC
GTACACAGCTACTATACGAGAAAGCACCAGCCTTATCCATATGGCAATTACAGCATCTTTGTCGCGGAAGTTGCCTCTAC
GACAAATGAAGCGCTCCTTGGCGAATATCTGCTGAACAATTTAGAGGATGAAAAACAGCGCTTATATATTCTCAACCATA
TGCTTGAGGGCTTCAGGGGAACGGTTTTCAGACAAACGATGTTCGCTGAATTTGAACATCTGATCCATACAAAGGCGCAA
GAAGGCGAGCCGCTTACACCTGAGCTTCTGACAAAGGTCTATTACGACCTGAATAAAAAGTATTTTGGAGACGGCATGGT
GATTGATAAGGAAATCGGCCTTGAATGGTCGCGTATTCCGCACTTCTATTACAATTACTATGTGTATCAGTATGCGACAG
GGTACAGCGCTGCCCAAGCATTAAGCAGCCAGATTTTGAAGGAAGGAAAGCCGGCGGTTGACCGTTATATTGACTTCCTG
AAAGCGGGAAGCTCACAATACCCGATTGATGTCCTGAAAAAAGCGGGTGTTGATATGACGTCTCCAGAGCCAATCGAAGC
CGCGTGCAAAATGTTTGAAGAAAAACTTGATGAAATGGAAGAGCTGCTGATGAAAGTCAAGCAGTCTTAA

Domains

Predicted by InterProScan.

Peptidase family M3 (Peptidase_M3)

(211-590)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

48.99

97.537

0.478