Detailed information    

insolico Bioinformatically predicted

Overview

Name   pptA   Type   Regulator
Locus tag   PUW21_RS05460 Genome accession   NZ_CP118167
Coordinates   1053022..1053765 (+) Length   247 a.a.
NCBI ID   WP_003233230.1    Uniprot ID   G4NRP4
Organism   Bacillus subtilis strain 21855     
Function   export ComS (predicted from homology)   
Competence regulation

Genomic Context

Location: 1048022..1058765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW21_RS05425 (PUW21_05425) yhaI 1048351..1048692 (+) 342 WP_009966921.1 YhaI family protein -
  PUW21_RS05430 (PUW21_05430) scoC 1048689..1049300 (-) 612 WP_003239501.1 HTH-type transcriptional regulator Hpr -
  PUW21_RS05435 (PUW21_05435) yhaH 1049478..1049834 (-) 357 WP_003245875.1 YtxH domain-containing protein -
  PUW21_RS05440 (PUW21_05440) - 1049964..1050155 (+) 192 WP_003233238.1 hypothetical protein -
  PUW21_RS05445 (PUW21_05445) trpP 1050229..1050747 (-) 519 WP_003233236.1 tryptophan transporter TrpP -
  PUW21_RS05450 (PUW21_05450) serC 1050871..1051950 (-) 1080 WP_069322489.1 3-phosphoserine/phosphohydroxythreonine transaminase -
  PUW21_RS05455 (PUW21_05455) hinT 1052097..1052534 (-) 438 WP_003233231.1 HIT family protein -
  PUW21_RS05460 (PUW21_05460) pptA 1053022..1053765 (+) 744 WP_003233230.1 ABC transporter ATP-binding protein EcsA Regulator
  PUW21_RS05465 (PUW21_05465) ecsB 1053758..1054984 (+) 1227 WP_003245169.1 ABC transporter permease EcsB -
  PUW21_RS05470 (PUW21_05470) ecsC 1055004..1055714 (+) 711 WP_003233225.1 EcsC family protein -
  PUW21_RS05475 (PUW21_05475) sndC 1055732..1056922 (-) 1191 WP_003245681.1 N-acetyl amino acid acetylase SndC -
  PUW21_RS05480 (PUW21_05480) - 1056995..1058386 (-) 1392 WP_003233218.1 hypothetical protein -

Sequence

Protein


Download         Length: 247 a.a.        Molecular weight: 27722.20 Da        Isoelectric Point: 5.9114

>NTDB_id=721476 PUW21_RS05460 WP_003233230.1 1053022..1053765(+) (pptA) [Bacillus subtilis strain 21855]
MSLLSVKDLTGGYTRNPVLKNVSFTLEPNQIVGLIGLNGAGKSTTIRHIIGLMDPHKGSIELNGKTFAEDPEGYRSQFTY
IPETPVLYEELTLMEHLELTAMAYGLSKETMEKRLPPLLKEFRMEKRLKWFPAHFSKGMKQKVMIMCAFLAEPALYIIDE
PFLGLDPLAINALLERMNEAKKGGASVLMSTHILATAERYCDSFIILHNGEVRARGTLSELREQFGMKDAALDDLYLELT
KEDAGHE

Nucleotide


Download         Length: 744 bp        

>NTDB_id=721476 PUW21_RS05460 WP_003233230.1 1053022..1053765(+) (pptA) [Bacillus subtilis strain 21855]
ATGTCTCTGCTATCGGTAAAAGACTTGACCGGCGGATATACAAGGAATCCGGTTTTAAAAAACGTATCATTCACCCTTGA
ACCGAATCAAATTGTCGGCTTAATCGGGCTGAATGGTGCTGGTAAAAGTACAACAATCAGACACATCATCGGGCTGATGG
ACCCGCATAAAGGTTCAATCGAATTAAACGGTAAAACGTTTGCTGAGGATCCGGAAGGCTACCGTTCACAATTTACCTAT
ATACCTGAAACACCTGTTTTATACGAAGAATTGACGCTGATGGAGCATCTTGAACTAACAGCCATGGCATATGGACTGTC
AAAAGAAACGATGGAGAAAAGGCTGCCTCCGCTACTAAAGGAATTCCGAATGGAAAAGAGGCTGAAGTGGTTCCCGGCCC
ATTTTTCTAAAGGAATGAAGCAGAAGGTTATGATTATGTGCGCATTTTTGGCAGAGCCTGCGCTCTACATTATTGATGAG
CCTTTTCTAGGGCTTGATCCGCTTGCCATTAACGCGCTGCTTGAACGGATGAATGAAGCGAAAAAAGGCGGGGCGAGCGT
GCTGATGTCAACACACATTTTGGCAACGGCAGAACGCTATTGCGATTCGTTTATTATTTTACATAACGGCGAGGTGCGGG
CGCGCGGCACGCTGTCAGAGCTCAGAGAGCAGTTTGGAATGAAGGACGCGGCGCTGGACGATTTGTATCTTGAGCTTACA
AAGGAAGACGCTGGCCATGAATAA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(19-162)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G4NRP4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pptA Streptococcus salivarius strain HSISS4

56.904

96.761

0.551
  pptA Streptococcus thermophilus LMD-9

56.485

96.761

0.547