Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   PUW46_RS07820 Genome accession   NZ_CP118044
Coordinates   1630206..1632476 (-) Length   756 a.a.
NCBI ID   WP_000595004.1    Uniprot ID   -
Organism   Staphylococcus aureus strain VSI56     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1625206..1637476
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW46_RS07800 (PUW46_07800) - 1625713..1626588 (-) 876 WP_000717800.1 N-acetylmuramoyl-L-alanine amidase -
  PUW46_RS07805 (PUW46_07805) dtd 1626585..1627037 (-) 453 WP_000869983.1 D-aminoacyl-tRNA deacylase -
  PUW46_RS07810 (PUW46_07810) - 1627049..1629238 (-) 2190 WP_001058587.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  PUW46_RS07815 (PUW46_07815) - 1629666..1630184 (-) 519 WP_000364542.1 adenine phosphoribosyltransferase -
  PUW46_RS07820 (PUW46_07820) recJ 1630206..1632476 (-) 2271 WP_000595004.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  PUW46_RS07825 (PUW46_07825) secDF 1632679..1634958 (-) 2280 WP_000749795.1 protein translocase subunit SecDF -
  PUW46_RS07830 (PUW46_07830) yajC 1635233..1635493 (-) 261 WP_001160682.1 preprotein translocase subunit YajC -
  PUW46_RS07835 (PUW46_07835) tgt 1635512..1636651 (-) 1140 WP_001112049.1 tRNA guanosine(34) transglycosylase Tgt -

Sequence

Protein


Download         Length: 756 a.a.        Molecular weight: 85547.82 Da        Isoelectric Point: 4.9063

>NTDB_id=720593 PUW46_RS07820 WP_000595004.1 1630206..1632476(-) (recJ) [Staphylococcus aureus strain VSI56]
MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQAIESIISDTDINHDALQLSDMTKTIERIKRAIANDE
KILVYGDYDADGVTSTTILVTTLQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFKALVAIGTIADLVSLTDENRSLV
KQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFIIGPRLNAVGRLDDASLACELLMTDEEEAAFLAEQVEHFNRERKD
IVATITEEAMAMAETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSIDQVSMFEILS
AHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVKQVDVLLTENDITIKNIRDMNRLRPFGTDFSR
PIFEMDDLSVSSVKAIGQQKNHLKLTLGESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQLIIQDIAMNE
QQILDYRSKRKSLPFTENDENIVMLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDLSNSLQQLQFSQLYIVLQ
HNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQHLSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSI
DSSKVYQLRQQRMDVEKQLLYQDFSEIKNWIKSQLS

Nucleotide


Download         Length: 2271 bp        

>NTDB_id=720593 PUW46_RS07820 WP_000595004.1 1630206..1632476(-) (recJ) [Staphylococcus aureus strain VSI56]
ATGATTAAACCGAAGTATAAGTGGAAATTAACTAAACCAGCTGAATACATAAGTGATGAATTAACATCAAAATTAAAGTT
AACGCCAATCGTAAAAAAAATATTAGAAAGTAAATCGATTATTGATGAACAAGCAATAGAATCCATCATTTCAGATACAG
ATATCAATCATGATGCACTGCAATTGAGCGATATGACTAAGACTATTGAAAGAATTAAAAGAGCGATAGCGAATGATGAG
AAAATCCTAGTTTATGGTGATTACGATGCCGACGGTGTTACCTCAACTACAATTTTAGTGACAACATTACAACTTTTAGG
TGCACAAGTAGGATGGCATATTCCTAACCGCTTTACCGAAGGATATGGACCAAACGAACTAGCATTTAGAAATGCTCATG
ATGAAGGCATAACATTAATCATAACTGTTGATAATGGCATACAAGGTCATAATGAAATTAAAATGGTTCAAGATTTAGGA
GTTGATGTCATTGTAACTGATCATCATGAAATTGGTAGTACATTACCAGAAGCATATGCAATCGTACATCCAATGCATCC
ATCGTTTAATTATCCGTTCCAACAATTGTGTGGTGCAGGTGTTGCGTATAAATTAGCACAAGCATTGATTGAAAATGTAC
CTGATTATTTTAAAGCATTAGTTGCGATAGGTACCATTGCGGATTTAGTATCCTTAACTGATGAAAATCGATCATTAGTG
AAACAAGGATTAAAAGTCTTGAATGATCAATGTCCGACCTCTGTTAAAGCATTGCTAAAAGAAGCGGGATACAATGACAA
TATTGATGAAGAAACAATTGGTTTTATTATTGGACCTAGACTTAATGCTGTAGGTCGTTTAGATGACGCATCATTAGCTT
GCGAATTGTTGATGACTGACGAAGAAGAAGCTGCATTTTTAGCAGAACAAGTTGAACATTTTAACCGTGAAAGAAAAGAT
ATTGTAGCAACTATCACAGAAGAAGCAATGGCTATGGCAGAAACGAAAGTTAAAAAGGGAGATTTATTTTTACTTTTAGC
AAAAGAAAATTGGCATGAAGGTGTCTTAGGTATTGTTGCTTCGAAAATTGTCGAAACATTTGCTTTGCCAACATTAATTT
TAAATATTGATAGGGAGCAAAATCATGCAAAAGGTTCCGCCAGATCAATTGATCAAGTGTCGATGTTTGAAATACTATCA
GCACATCAAGAGTTAATCGCTAAATTTGGCGGCCATCATATGGCTGCAGGTATGACGATGGATATTGAAAATATAGAATC
GTTAGCTGAAGGTTTAAATAAATGGATGAAAGAATTATCTAAAACAACTTCGTTAGATCCCGTAAAGCAAGTAGATGTAT
TATTAACAGAAAATGATATAACGATTAAAAATATTCGTGATATGAATCGTTTAAGACCCTTCGGAACAGATTTTTCTAGA
CCTATTTTTGAAATGGATGATTTATCAGTTTCTTCAGTTAAGGCGATAGGTCAACAAAAAAATCATCTAAAATTGACACT
TGGTGAAAGTAATATCGCCGCGCTATTTTGGCAAAATGGTCACTTAGAACCTGAGTTACAAGATGAACAGCCAATTAATA
TATTAGGATCTGTTCAAATCAACGAATGGAATGGTAATCAATCACCGCAATTAATTATTCAAGATATTGCGATGAATGAA
CAGCAAATATTAGATTATAGAAGTAAGCGAAAAAGTTTACCTTTTACAGAAAATGATGAAAATATTGTCATGCTTATTCA
TCCTAAAAGTGATAAAGTAAATGCGAATGAATATTATTATGGTGAAGAAATTAAACAACAAACTGATAAAGTAGTATTAA
GAGATTTACCAACGTCAATGGAAGACTTGTCTAATTCCTTGCAACAACTGCAATTTTCTCAACTTTATATAGTTTTGCAA
CATAATCATTCGATTTACTTCGATGGTATACCTAATATGGATATTTTTAAAAAGTGTTATAAAGCATTAATAACTAAACA
AGAAACAAATATCCAGAAAGAGGGTATGTTATTGTGTCAACATTTAAGTGTGAAACCAGATACACTTAAATTCATGTTGA
AAGTTTTCTTAGACTTAAAATTTGTAACACAAGAAGATGGTTTAATTCGAATCAATCAACAACCTGATAAAAGATCGATT
GATTCCAGCAAAGTATATCAATTAAGACAACAACGTATGGATGTTGAAAAGCAATTATTATATCAAGATTTTTCAGAAAT
AAAAAATTGGATAAAGTCACAATTGTCGTGA

Domains

Predicted by InterProScan.

RecJ OB domain (RecJ_OB)

(451-555)

DHH family (DHH)

(81-224)

DHHA1 domain (DHHA1)

(311-435)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(562-751)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

38.015

100

0.39
  recJ Streptococcus pneumoniae strain D39V

38.026

100

0.382