Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   PUW57_RS06640 Genome accession   NZ_CP118043
Coordinates   1266038..1268236 (-) Length   732 a.a.
NCBI ID   WP_000622237.1    Uniprot ID   Q8DZA1
Organism   Streptococcus agalactiae strain VSI63     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1261038..1273236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW57_RS06620 (PUW57_06620) - 1261096..1261779 (-) 684 WP_000365673.1 tRNA (adenine(22)-N(1))-methyltransferase TrmK -
  PUW57_RS06625 (PUW57_06625) - 1261883..1262563 (-) 681 WP_000221656.1 DnaD domain-containing protein -
  PUW57_RS06630 (PUW57_06630) - 1262681..1263199 (-) 519 WP_000365343.1 adenine phosphoribosyltransferase -
  PUW57_RS06635 (PUW57_06635) - 1263322..1265886 (-) 2565 WP_001247505.1 GBS Bsp-like repeat-containing protein -
  PUW57_RS06640 (PUW57_06640) recJ 1266038..1268236 (-) 2199 WP_000622237.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  PUW57_RS06645 (PUW57_06645) - 1268233..1268994 (-) 762 WP_000124583.1 SDR family oxidoreductase -
  PUW57_RS06650 (PUW57_06650) rnz 1268996..1269924 (-) 929 Protein_1231 ribonuclease Z -
  PUW57_RS06655 (PUW57_06655) - 1269966..1270613 (-) 648 WP_000568321.1 cystathionine beta-lyase -
  PUW57_RS06660 (PUW57_06660) hflX 1270606..1271844 (-) 1239 WP_000573352.1 GTPase HflX -
  PUW57_RS06665 (PUW57_06665) miaA 1271935..1272825 (-) 891 WP_001226480.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  PUW57_RS06670 (PUW57_06670) - 1272936..1273112 (+) 177 WP_000239229.1 DUF3042 family protein -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 82278.94 Da        Isoelectric Point: 4.5538

>NTDB_id=720494 PUW57_RS06640 WP_000622237.1 1266038..1268236(-) (recJ) [Streptococcus agalactiae strain VSI63]
MISAKYSWVLNNQKPDAGFFEASKKEKISEAVASLIYSRGIKTSAELHHFLQTNLENLHDPYLLNDMDKAVNRIRRAIEN
NETILVYGDYDADGMTSASIMKEALDMMGAEVQVYLPNRFTDGYGPNQSVYKYFIEQQDVSLIITVDNGVAGHEAITYAQ
NQGVDVVVTDHHSMPADLPCAYAIIHPEHPDANYPFPYLAGCGVAFKVACALLETIPTEMLDLVAIGTIADMVSLTDENR
IMVKAGLEVMKDSERIGLQELISLSNIDLKTLNEETIGFKIAPQLNALGRLDDPNPAIELLTGFDDEESQAIAQMIDQKN
EERKEIVQTIFDQAMQMLDQTKPVQVLAKENWHPGVLGIVAGRILERTGQPVIVLNIEDGIAKGSARSVEALDIFQAFDQ
HRELFIAFGGHSGAAGMTLEESKVGDLSQVLCDYISKKQLDMSQKKTLTIDSELRFDELSLDTVRDFEKLAPFGMDNKKP
VFLLKDFKVSQARVMGQNGAHLKLKLEQDGQALDLVAFNMGSQLQEFQQAQHLELAVTLSVNQWNGATTLQLMLEDARVD
GIQLFDIRSKASSLPHGVPILSQEEQSKEVILLTVPDHPQELKQMTQGKQFDAIYFKNEIPKNYFISGYGTRDQFASLYK
TIYQFPEFDVRYKLKELSSYLHIPDILLIKMIQIFEELHFVTITEGIMTVNKEAEKRDISESQIYQELKETVKFQELMAL
GTPKEIYDFMMS

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=720494 PUW57_RS06640 WP_000622237.1 1266038..1268236(-) (recJ) [Streptococcus agalactiae strain VSI63]
ATGATATCTGCTAAGTATAGTTGGGTCCTCAATAACCAAAAGCCAGACGCTGGCTTTTTTGAGGCTTCAAAAAAAGAAAA
GATAAGTGAAGCGGTGGCTTCCTTAATTTATTCGCGAGGTATTAAGACATCAGCGGAATTACATCATTTTTTACAAACTA
ATTTGGAAAATCTCCATGATCCTTATTTGTTAAATGATATGGATAAAGCTGTTAATCGTATTAGACGAGCTATTGAAAAT
AATGAAACTATTTTAGTTTATGGAGACTATGATGCTGATGGAATGACATCAGCATCAATCATGAAAGAAGCATTAGATAT
GATGGGAGCTGAAGTCCAGGTTTATCTCCCAAATCGCTTTACAGATGGTTACGGTCCAAATCAAAGTGTTTATAAATATT
TTATTGAACAACAAGATGTTTCATTAATCATTACGGTTGATAATGGTGTGGCAGGGCATGAAGCAATTACATATGCTCAA
AATCAAGGTGTGGACGTGGTAGTTACTGATCATCACAGTATGCCAGCAGATTTACCTTGTGCTTATGCTATTATTCACCC
AGAACATCCTGATGCTAATTATCCATTTCCTTATTTAGCAGGGTGTGGTGTTGCTTTCAAAGTAGCTTGTGCTCTCTTAG
AAACAATCCCGACAGAAATGTTGGATTTGGTTGCTATTGGGACGATTGCAGATATGGTTAGCCTAACTGATGAAAATCGT
ATCATGGTAAAAGCTGGACTTGAAGTTATGAAAGATAGTGAAAGAATAGGCTTACAGGAGCTAATATCTTTATCAAATAT
TGATTTAAAAACACTAAATGAAGAAACTATTGGATTTAAAATTGCTCCACAATTAAACGCTTTAGGAAGACTTGATGACC
CTAATCCAGCTATTGAATTACTAACAGGGTTTGATGATGAAGAATCTCAAGCAATAGCCCAAATGATTGATCAAAAAAAT
GAAGAGCGCAAAGAAATCGTTCAAACTATCTTTGACCAAGCTATGCAAATGCTTGATCAAACAAAGCCTGTACAAGTTTT
AGCAAAAGAAAATTGGCATCCAGGGGTTTTAGGTATTGTGGCGGGTCGTATTTTAGAGAGAACTGGACAACCTGTTATTG
TTTTGAATATTGAAGATGGTATTGCTAAAGGAAGTGCCAGAAGTGTAGAAGCACTTGATATTTTTCAAGCTTTTGACCAG
CATCGAGAATTATTCATTGCTTTTGGAGGTCACAGCGGAGCAGCAGGCATGACTTTAGAGGAAAGTAAAGTAGGTGATCT
TTCTCAAGTTTTATGTGACTATATCTCTAAAAAACAATTGGACATGAGTCAGAAAAAAACTCTGACAATTGATAGTGAGC
TACGTTTTGACGAACTTAGTTTAGACACTGTTAGAGATTTTGAAAAGTTAGCGCCATTTGGAATGGATAATAAAAAACCA
GTCTTTTTGTTAAAAGATTTTAAAGTGAGTCAAGCCAGAGTCATGGGGCAAAACGGTGCTCATCTGAAATTAAAACTAGA
ACAAGATGGACAAGCACTTGATTTGGTAGCATTTAATATGGGGAGTCAACTACAAGAGTTTCAACAAGCACAACATTTGG
AGTTAGCTGTCACTTTATCTGTTAATCAATGGAATGGTGCCACAACCCTACAATTAATGTTGGAGGATGCGCGTGTAGAT
GGTATACAATTATTTGATATTAGAAGCAAAGCAAGTAGCTTACCTCATGGAGTGCCAATCCTGTCTCAAGAAGAGCAGAG
CAAAGAAGTTATTCTTTTGACTGTTCCCGATCATCCACAAGAATTAAAACAAATGACTCAAGGTAAACAGTTTGATGCTA
TCTATTTTAAAAATGAGATACCAAAAAATTATTTTATATCTGGTTATGGTACACGTGATCAATTTGCTAGTTTATATAAA
ACAATATATCAATTCCCAGAATTTGACGTTCGTTATAAGTTAAAAGAGCTCAGTTCCTACCTTCATATTCCTGATATTTT
ATTAATTAAAATGATTCAAATTTTTGAAGAACTACATTTTGTTACGATTACAGAGGGGATTATGACAGTCAATAAAGAAG
CAGAAAAGCGAGATATCTCTGAGAGTCAAATTTATCAAGAATTAAAAGAGACAGTTAAGTTTCAAGAATTAATGGCTTTA
GGGACACCTAAAGAAATATATGATTTTATGATGAGTTGA

Domains

Predicted by InterProScan.

RecJ OB domain (RecJ_OB)

(450-556)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(583-726)

DHH family (DHH)

(83-228)

DHHA1 domain (DHHA1)

(317-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DZA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

64.858

100

0.653
  recJ Bacillus subtilis subsp. subtilis str. 168

35.881

100

0.378