Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PFJ30894_RS05000 Genome accession   NZ_AP019004
Coordinates   1042130..1043488 (+) Length   452 a.a.
NCBI ID   WP_021717359.1    Uniprot ID   -
Organism   Phascolarctobacterium faecium strain JCM 30894     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1037130..1048488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFJ30894_RS04985 (PFJ30894_01022) - 1037673..1038848 (+) 1176 WP_021717356.1 FprA family A-type flavoprotein -
  PFJ30894_RS04990 (PFJ30894_01023) - 1039030..1039443 (+) 414 WP_021717357.1 CtsR family transcriptional regulator -
  PFJ30894_RS04995 (PFJ30894_01024) - 1039650..1042130 (+) 2481 WP_125669936.1 ATP-dependent Clp protease ATP-binding subunit -
  PFJ30894_RS05000 (PFJ30894_01025) radA 1042130..1043488 (+) 1359 WP_021717359.1 DNA repair protein RadA Machinery gene
  PFJ30894_RS05005 (PFJ30894_01026) - 1043666..1044145 (+) 480 WP_021717360.1 CarD family transcriptional regulator -
  PFJ30894_RS05010 (PFJ30894_01027) - 1044256..1045380 (+) 1125 WP_021717361.1 PIN/TRAM domain-containing protein -
  PFJ30894_RS05015 (PFJ30894_01028) ispD 1045392..1046102 (+) 711 WP_125669200.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  PFJ30894_RS05020 (PFJ30894_01029) ispF 1046099..1046602 (+) 504 WP_113077267.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  PFJ30894_RS05025 (PFJ30894_01030) gltX 1046749..1048221 (+) 1473 WP_021717364.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 48980.68 Da        Isoelectric Point: 7.5393

>NTDB_id=72022 PFJ30894_RS05000 WP_021717359.1 1042130..1043488(+) (radA) [Phascolarctobacterium faecium strain JCM 30894]
MAKPKIRFVCQSCGYVTAKWLGKCPECGQWDSFVEEMDVPERPGQSYLPVGETAKPKPLHQVAEQSLSRLQTGIREFDRV
LGGGIVPGSLVLLGGDPGIGKSTMLLDAGMKFSRNNIKVLYVSGEESEAQTAMRARRLGSSGENLLVMTATDLNIILLQA
QEVQPALLVIDSIQTMYNSELPTAAGSVGQVRECTAKLLRFAKTSGIAVLIIGHVTKDGNIAGPRLLEHMVDVVLQFEGD
RSYSFRVLRALKNRFGSTNESGIFSMEASGLQEISNPAGLFLEERSNDASGSVVCACLEGNRPILVEVQALVAKTPYGMP
RRTAVGFDYNRVTMLLAILERRLGMDFGAYDAYVNVVGGMRVTEPAADLAVAAALVSSYRNRPIPHGVLVMGEVGLTGEV
RRIGSPERRIKDAMNLGFKRFVLPMGKLSLDEPCNGIVQVKTLEEALDKVFA

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=72022 PFJ30894_RS05000 WP_021717359.1 1042130..1043488(+) (radA) [Phascolarctobacterium faecium strain JCM 30894]
ATGGCAAAACCGAAAATACGTTTCGTTTGTCAGTCGTGTGGTTATGTAACGGCTAAATGGTTGGGAAAGTGTCCGGAATG
TGGTCAATGGGACAGCTTTGTCGAAGAAATGGACGTGCCTGAACGTCCTGGACAGAGTTACCTGCCTGTAGGCGAAACCG
CTAAGCCCAAACCGTTGCATCAGGTGGCGGAACAGTCCCTCAGTCGTCTGCAGACAGGTATCCGTGAATTTGATCGTGTG
CTTGGGGGAGGGATTGTTCCCGGCAGCCTGGTTTTGTTGGGCGGAGACCCGGGCATTGGCAAGTCGACTATGCTTTTGGA
TGCCGGCATGAAGTTTAGCCGGAATAATATAAAAGTTTTGTATGTTTCTGGTGAAGAATCAGAAGCGCAAACAGCAATGC
GGGCGAGACGTTTAGGCAGCAGCGGTGAAAACCTGCTGGTGATGACGGCTACCGATTTGAATATTATTTTACTGCAGGCA
CAGGAAGTGCAGCCGGCATTGTTGGTAATTGATTCTATTCAGACTATGTACAACAGCGAGCTGCCTACTGCAGCCGGCAG
TGTCGGACAGGTGCGCGAGTGTACGGCGAAATTGCTGCGTTTTGCAAAAACCAGCGGCATTGCCGTGCTTATTATCGGTC
ATGTGACTAAAGACGGTAATATTGCTGGCCCACGATTGCTTGAACATATGGTCGACGTGGTGCTGCAATTTGAAGGGGAT
AGATCCTATTCTTTTCGGGTGCTGCGTGCCTTGAAAAACCGTTTTGGCTCTACAAATGAAAGCGGTATTTTTTCTATGGA
AGCCAGTGGGCTGCAGGAAATAAGTAATCCAGCAGGATTATTCCTGGAAGAACGCAGTAACGATGCTTCCGGTTCAGTTG
TTTGTGCTTGCCTGGAAGGTAACAGGCCTATTTTAGTTGAAGTGCAGGCTTTGGTTGCCAAAACACCTTATGGGATGCCC
CGACGCACTGCTGTTGGATTTGATTATAATCGGGTCACTATGCTTTTGGCGATTTTGGAACGGCGCTTAGGAATGGATTT
TGGCGCTTACGATGCTTACGTCAATGTGGTTGGCGGGATGAGAGTCACTGAACCAGCTGCAGATCTGGCTGTGGCTGCTG
CGCTGGTGTCCAGTTATCGTAATCGGCCGATTCCGCATGGCGTATTAGTCATGGGTGAAGTGGGGCTTACAGGAGAAGTG
CGACGAATAGGTTCGCCGGAAAGGCGTATCAAAGATGCTATGAATCTTGGCTTTAAAAGATTTGTTTTGCCAATGGGCAA
GCTGTCATTGGATGAACCCTGCAATGGTATTGTGCAGGTTAAAACTTTGGAAGAAGCGCTGGATAAGGTTTTTGCTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-219)

Subunit ChlI of Mg-chelatase (ChlI)

(323-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

51.656

100

0.518
  radA Streptococcus mitis NCTC 12261

50.22

100

0.504
  radA Streptococcus mitis SK321

50.22

100

0.504
  radA Streptococcus pneumoniae Rx1

50

100

0.502
  radA Streptococcus pneumoniae D39

50

100

0.502
  radA Streptococcus pneumoniae R6

50

100

0.502
  radA Streptococcus pneumoniae TIGR4

50

100

0.502

Multiple sequence alignment