Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PUN50_RS01985 Genome accession   NZ_CP117988
Coordinates   429535..431010 (-) Length   491 a.a.
NCBI ID   WP_005432834.1    Uniprot ID   -
Organism   Vibrio campbellii strain 20220413_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 424535..436010
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN50_RS01955 (PUN50_01955) pmbA 424670..426013 (+) 1344 WP_274290792.1 metalloprotease PmbA -
  PUN50_RS01960 (PUN50_01960) mgtE 426097..427452 (-) 1356 WP_010450444.1 magnesium transporter -
  PUN50_RS01965 (PUN50_01965) - 427619..427894 (-) 276 WP_005432843.1 HPr family phosphocarrier protein -
  PUN50_RS01970 (PUN50_01970) rapZ 427897..428760 (-) 864 WP_005432858.1 RNase adapter RapZ -
  PUN50_RS01975 (PUN50_01975) ptsN 428775..429221 (-) 447 WP_005432846.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PUN50_RS01980 (PUN50_01980) hpf 429224..429511 (-) 288 WP_005432838.1 ribosome hibernation promoting factor -
  PUN50_RS01985 (PUN50_01985) rpoN/rpoN1 429535..431010 (-) 1476 WP_005432834.1 RNA polymerase factor sigma-54 Machinery gene
  PUN50_RS01990 (PUN50_01990) lptB 431061..431786 (-) 726 WP_005432855.1 LPS export ABC transporter ATP-binding protein -
  PUN50_RS01995 (PUN50_01995) lptA 431788..432282 (-) 495 WP_005432860.1 lipopolysaccharide transport periplasmic protein LptA -
  PUN50_RS02000 (PUN50_02000) lptC 432263..432826 (-) 564 WP_005535416.1 LPS export ABC transporter periplasmic protein LptC -
  PUN50_RS02005 (PUN50_02005) kdsD 432917..433888 (-) 972 WP_005535415.1 arabinose-5-phosphate isomerase KdsD -
  PUN50_RS02010 (PUN50_02010) - 433924..434889 (-) 966 WP_274290793.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 491 a.a.        Molecular weight: 55074.67 Da        Isoelectric Point: 4.3082

>NTDB_id=720165 PUN50_RS01985 WP_005432834.1 429535..431010(-) (rpoN/rpoN1) [Vibrio campbellii strain 20220413_1]
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALDSNPLLEVEEGHDEPQANGEDKSASESADNSANEANDAS
EPDLPDSSDVIEKSEISSELEIDTTWDDVYSANTGSTGLALDDDMPVYQGETTESLHDYLMWQLDLTPFSETDRTIALAI
IDAIDDYGYLTLSPEEIHESFDNEEVELDEVEAVRKRIQQFDPLGVASRNLQECLLLQLATFPEDTPWLAEAKMVLSDHI
DHLGNRDYKLVIKETKLKEADLREVLKLIQQLDPRPGSRITPDDTEYVIPDVSVFKDHGKWTVSINPDSIPKLKVNQQYA
QLGKGNSADSQYIRSNLQEAKWLIKSLESRNETLLKVARCIVEHQQDFFEYGEEAMKPMVLNDVALDVDMHESTISRVTT
QKFMHTPRGIFELKYFFSSHVSTDNGGECSSTAIRALIKKLVAAENTAKPLSDSKIAALLADQGIQVARRTIAKYRESLG
IAPSSQRKRLL

Nucleotide


Download         Length: 1476 bp        

>NTDB_id=720165 PUN50_RS01985 WP_005432834.1 429535..431010(-) (rpoN/rpoN1) [Vibrio campbellii strain 20220413_1]
ATGAAACCTTCATTACAACTCAAGCTAGGTCAACAGTTAGCCATGACGCCACAACTGCAGCAAGCGATTCGTTTGTTGCA
ATTGTCGACGCTCGATCTTCAACAAGAAATCCAAGAAGCGTTGGACTCCAACCCGCTACTGGAAGTTGAAGAAGGCCACG
ATGAGCCTCAAGCAAACGGTGAAGACAAATCAGCGTCTGAATCTGCTGATAACAGTGCGAACGAAGCTAACGATGCCTCA
GAACCCGACCTTCCAGATAGCTCAGACGTGATTGAAAAATCTGAAATCAGCTCTGAGCTAGAAATCGATACCACTTGGGA
TGACGTATACAGCGCAAACACGGGCAGCACAGGCCTAGCGCTAGATGATGACATGCCCGTCTACCAAGGTGAGACCACTG
AATCGCTGCATGATTACCTTATGTGGCAGTTAGACTTAACGCCTTTCAGTGAAACCGACCGCACCATTGCCCTCGCGATC
ATCGATGCCATCGACGACTACGGCTACCTAACCCTATCCCCAGAAGAAATTCACGAGAGCTTCGACAACGAAGAAGTGGA
ATTGGATGAAGTAGAAGCGGTACGTAAGCGTATTCAGCAATTTGACCCGCTCGGTGTAGCCTCTCGCAATCTGCAAGAGT
GCCTATTGCTACAACTGGCAACTTTCCCTGAAGACACGCCATGGCTTGCTGAGGCGAAAATGGTGTTAAGCGATCACATC
GACCACCTTGGCAATCGTGACTACAAGCTGGTCATCAAAGAGACTAAGCTTAAAGAAGCGGACTTGCGTGAAGTATTGAA
GTTGATTCAACAACTTGATCCTCGCCCAGGTAGTCGTATCACACCCGATGACACTGAATACGTCATTCCGGATGTGTCCG
TATTTAAAGATCATGGTAAGTGGACCGTCTCCATAAACCCTGACAGCATTCCGAAACTAAAAGTAAATCAACAATATGCG
CAACTAGGCAAAGGAAACAGTGCGGATAGCCAGTACATTCGCAGCAATTTGCAAGAGGCAAAATGGCTGATTAAGAGCCT
AGAAAGCAGAAACGAGACGCTTCTCAAAGTTGCAAGATGTATTGTTGAACATCAACAAGATTTCTTCGAGTATGGTGAAG
AAGCCATGAAACCAATGGTGCTAAACGACGTAGCATTGGATGTGGACATGCATGAATCGACAATTTCTCGTGTAACAACA
CAGAAGTTTATGCATACCCCACGTGGCATTTTTGAATTGAAGTACTTCTTCTCTAGCCATGTTAGTACAGACAATGGTGG
AGAGTGTTCGTCCACAGCAATTCGCGCACTCATCAAAAAGTTGGTCGCAGCGGAGAATACCGCTAAGCCACTGAGTGATA
GCAAAATTGCTGCTCTTCTGGCTGACCAGGGGATTCAAGTCGCGAGACGTACGATAGCGAAATATCGTGAATCCTTGGGT
ATCGCCCCTTCGAGTCAGCGCAAACGCCTACTTTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(132-319)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(331-489)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

43.936

100

0.45